LOCUS       BBa_J06916              1936 bp    DNA     linear       09-May-2024
DEFINITION  Lambda cI QPI regulated by LacI with EYFP
ACCESSION   BBa_J06916
VERSION     BBa_J06916.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..831
                     /label=BBa_C0051
     stem_loop       876..919
                     /label=stem_loop
     misc_feature    865..944
                     /label=BBa_B0010
     terminator      865..944
                     /label=BBa_B0010
     stem_loop       960..979
                     /label=T7 TE
     misc_feature    986..986
                     /label=stop
     polyA_site      980..993
                     /label=polya
     misc_feature    953..993
                     /label=BBa_B0012
     terminator      953..993
                     /label=BBa_B0012
     promoter        1039..1044
                     /label=-10
     promoter        1016..1021
                     /label=-35
     misc_feature    1026..1042
                     /label=OR1
     misc_feature    1002..1018
                     /label=OR2
     misc_feature    1002..1050
                     /label=BBa_R0051
     promoter        1002..1050
                     /label=BBa_R0051
     misc_feature    1063..1066
                     /label=conserved
     RBS             1059..1070
                     /label=BBa_B0034
     CDS             1077..1799
                     /label=BBa_E0030
     stem_loop       1819..1862
                     /label=stem_loop
     misc_feature    1808..1887
                     /label=BBa_B0010
     terminator      1808..1887
                     /label=BBa_B0010
     stem_loop       1903..1922
                     /label=T7 TE
     misc_feature    1929..1929
                     /label=stop
     polyA_site      1923..1936
                     /label=polya
     misc_feature    1896..1936
                     /label=BBa_B0012
     terminator      1896..1936
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      961 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaacaccgt gcgtgttgac
     1021 tattttacct ctggcggtga taatggttgc tactagagaa agaggagaaa tactagatgg
     1081 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1141 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1201 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1261 tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc
     1321 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1381 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1441 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1501 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1561 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1621 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1681 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1741 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat
     1801 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1861 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1921 cctttctgcg tttata
//