LOCUS BBa_J06954 2884 bp DNA linear 26-Dec-2024 DEFINITION 434 cI/lambda cI857 (E07700) RS flip-flop ACCESSION BBa_J06954 VERSION BBa_J06954.1 FEATURES Location/Qualifiers misc_feature 1..43 /label=OR2 misc_feature 8..21 /label=OR2 misc_feature 30..43 /label=OR1 promoter 1..6 /label=-35 promoter 24..29 /label=-10 promoter 2..7 /label=-35 misc_feature 35..37 /label=start misc_feature 1..46 /label=BBa_R0052 promoter 1..46 /label=BBa_R0052 misc_feature 59..62 /label=conserved RBS 55..66 /label=BBa_B0034 CDS 73..783 /label=cI857 misc_feature 784..816 /label=LVA misc_feature 320..320 /label=G->C misc_feature 424..424 /label=G->A (ind-) misc_feature 753..753 /label=C->G misc_feature 823..847 /label=barcode from lambda cI (BBa_C0051) CDS 73..847 /label=BBa_J06502 stem_loop 867..910 /label=stem_loop misc_feature 856..935 /label=BBa_B0010 terminator 856..935 /label=BBa_B0010 stem_loop 951..970 /label=T7 TE misc_feature 977..977 /label=stop polyA_site 971..984 /label=polya misc_feature 944..984 /label=BBa_B0012 terminator 944..984 /label=BBa_B0012 promoter 1030..1035 /label=-10 promoter 1007..1012 /label=-35 misc_feature 1017..1033 /label=OR1 misc_feature 993..1009 /label=OR2 misc_feature 993..1041 /label=BBa_R0051 promoter 993..1041 /label=BBa_R0051 misc_feature 1054..1057 /label=conserved RBS 1050..1061 /label=BBa_B0034 CDS 1068..1736 /label=cI 434 misc_feature 1698..1736 /label=LVA misc_feature 1068..1736 /label=BBa_C0052 misc_feature 1737..1761 /label=Help:Barcodes CDS 1068..1736 /label=BBa_C0052 stem_loop 1781..1824 /label=stem_loop misc_feature 1770..1849 /label=BBa_B0010 terminator 1770..1849 /label=BBa_B0010 stem_loop 1865..1884 /label=T7 TE misc_feature 1891..1891 /label=stop polyA_site 1885..1898 /label=polya misc_feature 1858..1898 /label=BBa_B0012 terminator 1858..1898 /label=BBa_B0012 misc_feature 1907..1949 /label=OR2 misc_feature 1914..1927 /label=OR2 misc_feature 1936..1949 /label=OR1 promoter 1907..1912 /label=-35 promoter 1930..1935 /label=-10 promoter 1908..1913 /label=-35 misc_feature 1941..1943 /label=start misc_feature 1907..1952 /label=BBa_R0052 promoter 1907..1952 /label=BBa_R0052 misc_feature 1965..1968 /label=conserved RBS 1961..1972 /label=BBa_B0034 misc_feature 1979..1993 /label=MATSG linker misc_feature 2702..2716 /label=GSGTA linker CDS 1979..2716 /label=mCherry misc_feature 2723..2747 /label=Help:Barcodes misc_feature 1979..2722 /label=BBa_E2060 stem_loop 2767..2810 /label=stem_loop misc_feature 2756..2835 /label=BBa_B0010 terminator 2756..2835 /label=BBa_B0010 stem_loop 2851..2870 /label=T7 TE misc_feature 2877..2877 /label=stop polyA_site 2871..2884 /label=polya misc_feature 2844..2884 /label=BBa_B0012 terminator 2844..2884 /label=BBa_B0012 ORIGIN 1 ttgacaaaca agatacattg tatgaaaata caagaaagtt tgttgatact agagaaagag 61 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca 121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct 181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat 241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa 301 tttagccctt caatcgccac agaaatctac gagatgtatg aagcggttag tatgcagccg 361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca 421 cctaagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa 481 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc 541 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag 601 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc 661 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc 721 aatgagagtt gttccgttgt ggggaaagtt atggctagtc agtggcctga agagacgttt 781 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt 841 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 901 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 961 cgggtgggcc tttctgcgtt tatatactag agtaacaccg tgcgtgttga ctattttacc 1021 tctggcggtg ataatggttg ctactagaga aagaggagaa atactagatg agtatttctt 1081 ccagggtaaa aagcaaaaga atccagcttg gacttaacca ggctgaactt gctcaaaagg 1141 tggggactac ccagcagtct atagagcagc tcgaaaacgg taaaactaag cgaccacgct 1201 ttttaccaga acttgcgtca gctcttggcg taagtgttga ctggctgctc aatggcacct 1261 ctgattcgaa tgttagattt gttgggcacg ttgagcccaa agggaaatat ccattgatta 1321 gcatggttag agctggttcg tggtgtgaag cttgtgaacc ctacgatatc aaggacattg 1381 atgaatggta tgacagtgac gttaacttat taggcaatgg attctggctg aaggttgaag 1441 gtgattccat gacctcacct gtaggtcaaa gcatccctga aggtcatatg gtgttagtag 1501 atactggacg ggagccagtg aatggaagcc ttgttgtagc caaactgact gacgcgaacg 1561 aagcaacatt caagaaactg gtcatagatg gcggtcagaa gtacctgaaa ggcctgaatc 1621 cttcatggcc tatgactcct atcaacggaa actgcaagat tatcggtgtt gtcgtggaag 1681 cgagggtaaa attcgtagct gcaaacgacg aaaactacgc tttagtagct taataactct 1741 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc 1801 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1861 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga caaacaagat 1921 acattgtatg aaaatacaag aaagtttgtt gatactagag aaagaggaga aatactagat 1981 ggcaactagc ggcatggtta gtaaaggaga agaaaataac atggcaatca ttaaggagtt 2041 catgagattc aaagttcaca tggaaggttc tgtaaatgga catgaatttg aaatagaagg 2101 tgaaggagaa ggaaggcctt atgaaggaac ccaaaccgcg aagctaaaag ttactaaggg 2161 tggcccatta ccatttgcat gggatatcct tagccctcaa ttcatgtatg ggtcaaaggc 2221 ttatgtcaag caccccgccg acattccaga ctatctaaag ttatcttttc ccgaagggtt 2281 taagtgggag cgtgtgatga acttcgaaga cggtggcgtg gtaacagtga ctcaggattc 2341 gtccctgcaa gatggtgaat ttatctacaa agtcaaatta agaggaacta actttccatc 2401 tgacggcccg gttatgcaaa aaaagacaat gggctgggag gcctcctcag aacgaatgta 2461 ccctgaagat ggtgccttga agggtgagat taaacaaaga ttgaaattga aagatggtgg 2521 acattatgac gctgaggtta aaacgacata caaagctaag aaacctgtcc agctcccagg 2581 tgcttacaat gtaaatataa aacttgatat tacatcacat aatgaagatt atacgatagt 2641 tgaacaatac gaaagggctg aggggagaca tagtactggt ggcatggatg aactatacaa 2701 aggttctggt accgcataat aacactgata gtgctagtgt agatcactac tagagccagg 2761 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2821 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2881 tata //