##gff-version 3
##sequence-region BBa_J07001 1 3229
BBa_J07001	.	CDS	2370	3092	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J07001	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_J07001	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J07001	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J07001	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J07001	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J07001	.	terminator	1242	1321	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J07001	.	engineered_region	1242	1321	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07001	.	stem_loop	1253	1296	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07001	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_J07001	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_J07001	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_J07001	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_J07001	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_J07001	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_J07001	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_J07001	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_J07001	.	terminator	3189	3229	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J07001	.	engineered_region	3189	3229	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07001	.	stem_loop	3196	3215	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07001	.	polyA_site	3216	3229	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07001	.	stop_codon	3222	3222	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07001	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J07001	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07001	.	CDS	1460	2119	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_J07001	.	CDS	1463	2079	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J07001	.	engineered_tag	2120	2144	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J07001	.	tag	2080	2113	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J07001	.	ribosome_entry_site	2352	2363	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J07001	.	conserved_region	2356	2359	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07001	.	promoter	2290	2343	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J07001	.	promoter	2332	2337	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07001	.	promoter	2309	2314	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J07001	.	engineered_region	2290	2343	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07001	.	non_covalent_binding_site	2315	2333	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07001	.	non_covalent_binding_site	2290	2308	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07001	.	terminator	3101	3180	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J07001	.	engineered_region	3101	3180	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07001	.	stem_loop	3112	3155	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07001	.	terminator	2153	2232	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J07001	.	engineered_region	2153	2232	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07001	.	stem_loop	2164	2207	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07001	.	terminator	1330	1370	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J07001	.	engineered_region	1330	1370	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07001	.	stem_loop	1337	1356	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07001	.	polyA_site	1357	1370	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07001	.	stop_codon	1363	1363	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07001	.	terminator	2241	2281	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J07001	.	engineered_region	2241	2281	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07001	.	stem_loop	2248	2267	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07001	.	polyA_site	2268	2281	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07001	.	stop_codon	2274	2274	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07001	.	ribosome_entry_site	1442	1453	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J07001	.	conserved_region	1446	1449	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07001	.	promoter	1379	1432	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J07001	.	engineered_region	1379	1432	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J07001	.	operator	1404	1420	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J07001	.	promoter	1421	1426	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J07001	.	operator	1381	1397	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J07001	.	promoter	1398	1403	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
>BBa_J07001
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaaga
tactgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcatta
gagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacat
tgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcactt
ttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcat
cgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttt
tatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtatt
ggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaa
gctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaa
aacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtc
cctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactaga
tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaa
gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag
ctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatga
agctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa
ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag
gaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcaga
agaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca
gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagac
cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt
acaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata