LOCUS BBa_J07002 3314 bp DNA linear 26-Dec-2024 DEFINITION abandoned test construct ACCESSION BBa_J07002 VERSION BBa_J07002.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 1446..1449 /label=conserved RBS 1442..1453 /label=BBa_B0034 CDS 1463..2170 /label=cI lambda misc_feature 2171..2203 /label=LVA misc_feature 2210..2234 /label=Help:Barcodes CDS 1460..2209 /label=BBa_C0051 stem_loop 2254..2297 /label=stem_loop misc_feature 2243..2322 /label=BBa_B0010 terminator 2243..2322 /label=BBa_B0010 stem_loop 2338..2357 /label=T7 TE misc_feature 2364..2364 /label=stop polyA_site 2358..2371 /label=polya misc_feature 2331..2371 /label=BBa_B0012 terminator 2331..2371 /label=BBa_B0012 promoter 2417..2422 /label=-10 promoter 2394..2399 /label=-35 misc_feature 2404..2420 /label=OR1 misc_feature 2380..2396 /label=OR2 misc_feature 2380..2428 /label=BBa_R0051 promoter 2380..2428 /label=BBa_R0051 misc_feature 2441..2444 /label=conserved RBS 2437..2448 /label=BBa_B0034 CDS 2455..3177 /label=BBa_E0030 stem_loop 3197..3240 /label=stem_loop misc_feature 3186..3265 /label=BBa_B0010 terminator 3186..3265 /label=BBa_B0010 stem_loop 3281..3300 /label=T7 TE misc_feature 3307..3307 /label=stop polyA_site 3301..3314 /label=polya misc_feature 3274..3314 /label=BBa_B0012 terminator 3274..3314 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga 1501 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca 1561 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg 1621 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt 1681 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat 1741 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat 1801 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac 1861 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc 1921 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc 1981 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa 2041 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat 2101 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga 2161 gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 2221 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 2281 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 2341 tcaccttcgg gtgggccttt ctgcgtttat atactagagt aacaccgtgc gtgttgacta 2401 ttttacctct ggcggtgata atggttgcta ctagagaaag aggagaaata ctagatggtg 2461 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 2521 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 2581 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 2641 accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac 2701 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 2761 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 2821 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 2881 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 2941 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 3001 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 3061 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 3121 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac 3181 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3241 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 3301 tttctgcgtt tata //