LOCUS BBa_J07003 3295 bp DNA linear 22-Nov-2024 DEFINITION abandoned test construct ACCESSION BBa_J07003 VERSION BBa_J07003.1 FEATURES Location/Qualifiers promoter 6..28 /label=consensus sequence promoter 1..35 /label=BBa_Z0251 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1151..1189 /label=LVA CDS 62..1189 /label=lacI-LVA misc_feature 62..1189 /label=BBa_C0012 misc_feature 1190..1214 /label=Help:Barcodes CDS 62..1189 /label=BBa_C0012 stem_loop 1234..1277 /label=stem_loop misc_feature 1223..1302 /label=BBa_B0010 terminator 1223..1302 /label=BBa_B0010 stem_loop 1318..1337 /label=T7 TE misc_feature 1344..1344 /label=stop polyA_site 1338..1351 /label=polya misc_feature 1311..1351 /label=BBa_B0012 terminator 1311..1351 /label=BBa_B0012 misc_feature 1362..1378 /label=lac O1 promoter 1379..1384 /label=-35 misc_feature 1385..1401 /label=lac O1 promoter 1402..1407 /label=-10 misc_feature 1360..1413 /label=BBa_R0011 promoter 1360..1413 /label=BBa_R0011 misc_feature 1427..1430 /label=conserved RBS 1423..1434 /label=BBa_B0034 CDS 1444..2151 /label=cI lambda misc_feature 2152..2184 /label=LVA misc_feature 2191..2215 /label=Help:Barcodes CDS 1441..2190 /label=BBa_C0051 stem_loop 2235..2278 /label=stem_loop misc_feature 2224..2303 /label=BBa_B0010 terminator 2224..2303 /label=BBa_B0010 stem_loop 2319..2338 /label=T7 TE misc_feature 2345..2345 /label=stop polyA_site 2339..2352 /label=polya misc_feature 2312..2352 /label=BBa_B0012 terminator 2312..2352 /label=BBa_B0012 promoter 2398..2403 /label=-10 promoter 2375..2380 /label=-35 misc_feature 2385..2401 /label=OR1 misc_feature 2361..2377 /label=OR2 misc_feature 2361..2409 /label=BBa_R0051 promoter 2361..2409 /label=BBa_R0051 misc_feature 2422..2425 /label=conserved RBS 2418..2429 /label=BBa_B0034 CDS 2436..3158 /label=BBa_E0030 stem_loop 3178..3221 /label=stem_loop misc_feature 3167..3246 /label=BBa_B0010 terminator 3167..3246 /label=BBa_B0010 stem_loop 3262..3281 /label=T7 TE misc_feature 3288..3288 /label=stop polyA_site 3282..3295 /label=polya misc_feature 3255..3295 /label=BBa_B0012 terminator 3255..3295 /label=BBa_B0012 ORIGIN 1 gaaattaata cgactcacta tagggagacc acaactacta gagaaagagg agaaatacta 61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt 1381 gacattgtga gcggataaca agatactgag cacatactag agaaagagga gaaatactag 1441 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa 1501 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag 1561 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct 1621 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca 1681 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt 1741 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga 1801 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct 1861 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc 1921 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc 1981 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt 2041 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt 2101 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgctgcaaac 2161 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta 2221 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 2281 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 2341 tctgcgttta tatactagag taacaccgtg cgtgttgact attttacctc tggcggtgat 2401 aatggttgct actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 2461 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 2521 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 2581 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc 2641 tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca 2701 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 2761 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 2821 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 2881 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 2941 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 3001 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga 3061 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 3121 ggatcactct cggcatggac gagctgtaca agtaataata ctagagccag gcatcaaata 3181 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3241 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //