##gff-version 3 ##sequence-region BBa_J07003 1 3295 BBa_J07003 . CDS 2436 3158 . + 0 ID=BBa_E0030;Name=BBa_E0030 BBa_J07003 . CDS 1441 2190 . + 0 ID=BBa_C0051;Name=BBa_C0051 BBa_J07003 . CDS 1444 2151 . + 0 ID=annotation23334;Name=cI lambda;Parent=BBa_C0051 BBa_J07003 . tag 2152 2184 . + 0 ID=annotation23335;Name=LVA;Parent=BBa_C0051 BBa_J07003 . engineered_tag 2191 2215 . + 0 ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051 BBa_J07003 . terminator 3167 3246 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J07003 . stem_loop 3178 3221 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J07003 . engineered_region 3167 3246 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J07003 . terminator 3255 3295 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J07003 . polyA_site 3282 3295 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J07003 . stem_loop 3262 3281 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J07003 . engineered_region 3255 3295 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J07003 . stop_codon 3288 3288 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J07003 . CDS 62 1189 . + 0 ID=BBa_C0012;Name=BBa_C0012 BBa_J07003 . engineered_region 62 1189 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_J07003 . CDS 62 1189 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_J07003 . engineered_tag 1190 1214 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_J07003 . tag 1151 1189 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_J07003 . promoter 1 35 . + 0 ID=BBa_Z0251;Name=BBa_Z0251 BBa_J07003 . promoter 6 28 . + 0 ID=annotation1476333;Name=consensus sequence;Parent=BBa_Z0251 BBa_J07003 . terminator 1311 1351 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J07003 . polyA_site 1338 1351 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J07003 . stem_loop 1318 1337 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J07003 . engineered_region 1311 1351 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J07003 . stop_codon 1344 1344 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J07003 . ribosome_entry_site 44 55 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J07003 . conserved_region 48 51 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J07003 . promoter 2361 2409 . + 0 ID=BBa_R0051;Name=BBa_R0051 BBa_J07003 . promoter 2375 2380 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_J07003 . non_covalent_binding_site 2385 2401 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_J07003 . non_covalent_binding_site 2361 2377 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_J07003 . engineered_region 2361 2409 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_J07003 . promoter 2398 2403 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_J07003 . terminator 2224 2303 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J07003 . stem_loop 2235 2278 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J07003 . engineered_region 2224 2303 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J07003 . terminator 1223 1302 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J07003 . stem_loop 1234 1277 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J07003 . engineered_region 1223 1302 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J07003 . promoter 1360 1413 . + 0 ID=BBa_R0011;Name=BBa_R0011 BBa_J07003 . operator 1362 1378 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_J07003 . engineered_region 1360 1413 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_J07003 . promoter 1379 1384 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_J07003 . operator 1385 1401 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_J07003 . promoter 1402 1407 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_J07003 . ribosome_entry_site 2418 2429 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J07003 . conserved_region 2422 2425 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J07003 . terminator 2312 2352 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J07003 . polyA_site 2339 2352 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J07003 . stem_loop 2319 2338 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J07003 . engineered_region 2312 2352 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J07003 . stop_codon 2345 2345 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J07003 . ribosome_entry_site 1423 1434 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J07003 . conserved_region 1427 1430 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 >BBa_J07003 gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatggtgaatgtgaaacca gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg cgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagag aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaag caatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcagg cgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagc gttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaa gtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtga tgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgacc gcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtg atttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttaca accactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtgg cctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagat cgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtg cgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaaggg cgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtcc ggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccct ggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgactt cttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgc gccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaaca tcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaa ggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatc ggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagc gcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataata ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata