LOCUS BBa_J07004 3210 bp DNA linear 26-Dec-2024 DEFINITION abandoned test construct ACCESSION BBa_J07004 VERSION BBa_J07004.1 FEATURES Location/Qualifiers promoter 6..28 /label=consensus sequence promoter 1..35 /label=BBa_Z0251 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1151..1189 /label=LVA CDS 62..1189 /label=lacI-LVA misc_feature 62..1189 /label=BBa_C0012 misc_feature 1190..1214 /label=Help:Barcodes CDS 62..1189 /label=BBa_C0012 stem_loop 1234..1277 /label=stem_loop misc_feature 1223..1302 /label=BBa_B0010 terminator 1223..1302 /label=BBa_B0010 stem_loop 1318..1337 /label=T7 TE misc_feature 1344..1344 /label=stop polyA_site 1338..1351 /label=polya misc_feature 1311..1351 /label=BBa_B0012 terminator 1311..1351 /label=BBa_B0012 misc_feature 1362..1378 /label=lac O1 promoter 1379..1384 /label=-35 misc_feature 1385..1401 /label=lac O1 promoter 1402..1407 /label=-10 misc_feature 1360..1413 /label=BBa_R0011 promoter 1360..1413 /label=BBa_R0011 misc_feature 1427..1430 /label=conserved RBS 1423..1434 /label=BBa_B0034 CDS 1444..2060 /label=tetR misc_feature 2061..2094 /label=SsrA misc_feature 2101..2125 /label=Help:Barcodes CDS 1441..2100 /label=BBa_C0040 stem_loop 2145..2188 /label=stem_loop misc_feature 2134..2213 /label=BBa_B0010 terminator 2134..2213 /label=BBa_B0010 stem_loop 2229..2248 /label=T7 TE misc_feature 2255..2255 /label=stop polyA_site 2249..2262 /label=polya misc_feature 2222..2262 /label=BBa_B0012 terminator 2222..2262 /label=BBa_B0012 misc_feature 2271..2289 /label=TetR 1 misc_feature 2271..2324 /label=BBa_R0040 promoter 2290..2295 /label=-35 misc_feature 2296..2314 /label=TetR 2 promoter 2313..2318 /label=-10 promoter 2271..2324 /label=BBa_R0040 misc_feature 2337..2340 /label=conserved RBS 2333..2344 /label=BBa_B0034 CDS 2351..3073 /label=BBa_E0020 stem_loop 3093..3136 /label=stem_loop misc_feature 3082..3161 /label=BBa_B0010 terminator 3082..3161 /label=BBa_B0010 stem_loop 3177..3196 /label=T7 TE misc_feature 3203..3203 /label=stop polyA_site 3197..3210 /label=polya misc_feature 3170..3210 /label=BBa_B0012 terminator 3170..3210 /label=BBa_B0012 ORIGIN 1 gaaattaata cgactcacta tagggagacc acaactacta gagaaagagg agaaatacta 61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt 1381 gacattgtga gcggataaca agatactgag cacatactag agaaagagga gaaatactag 1441 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc 1501 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca 1561 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta 1621 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt 1681 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat 1741 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta 1801 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt 1861 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca 1921 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa 1981 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa 2041 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa 2101 cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc 2161 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2221 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag 2281 tgatagagat tgacatccct atcagtgata gagatactga gcactactag agaaagagga 2341 gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 2401 cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga 2461 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 2521 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga 2581 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 2641 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 2701 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 2761 cctggggcac aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa 2821 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt 2881 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 2941 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 3001 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 3061 gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3121 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3181 caccttcggg tgggcctttc tgcgtttata //