##gff-version 3
##sequence-region BBa_J07004 1 3210
BBa_J07004	.	CDS	62	1189	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_J07004	.	engineered_region	62	1189	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J07004	.	CDS	62	1189	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J07004	.	engineered_tag	1190	1214	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J07004	.	tag	1151	1189	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J07004	.	terminator	1311	1351	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J07004	.	polyA_site	1338	1351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07004	.	stem_loop	1318	1337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07004	.	engineered_region	1311	1351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07004	.	stop_codon	1344	1344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07004	.	promoter	1360	1413	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J07004	.	operator	1362	1378	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J07004	.	engineered_region	1360	1413	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J07004	.	promoter	1379	1384	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J07004	.	operator	1385	1401	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J07004	.	promoter	1402	1407	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J07004	.	terminator	1223	1302	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J07004	.	stem_loop	1234	1277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07004	.	engineered_region	1223	1302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07004	.	promoter	1	35	.	+	0	ID=BBa_Z0251;Name=BBa_Z0251
BBa_J07004	.	promoter	6	28	.	+	0	ID=annotation1476333;Name=consensus sequence;Parent=BBa_Z0251
BBa_J07004	.	ribosome_entry_site	1423	1434	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J07004	.	conserved_region	1427	1430	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07004	.	terminator	3082	3161	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J07004	.	stem_loop	3093	3136	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07004	.	engineered_region	3082	3161	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07004	.	terminator	3170	3210	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J07004	.	polyA_site	3197	3210	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07004	.	stem_loop	3177	3196	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07004	.	engineered_region	3170	3210	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07004	.	stop_codon	3203	3203	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07004	.	promoter	2271	2324	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J07004	.	promoter	2290	2295	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J07004	.	promoter	2313	2318	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07004	.	non_covalent_binding_site	2271	2289	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07004	.	engineered_region	2271	2324	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07004	.	non_covalent_binding_site	2296	2314	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07004	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J07004	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07004	.	CDS	2351	3073	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_J07004	.	terminator	2134	2213	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J07004	.	stem_loop	2145	2188	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07004	.	engineered_region	2134	2213	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07004	.	ribosome_entry_site	2333	2344	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J07004	.	conserved_region	2337	2340	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07004	.	terminator	2222	2262	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J07004	.	polyA_site	2249	2262	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07004	.	stem_loop	2229	2248	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07004	.	engineered_region	2222	2262	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07004	.	stop_codon	2255	2255	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07004	.	CDS	1441	2100	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_J07004	.	CDS	1444	2060	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J07004	.	tag	2061	2094	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J07004	.	engineered_tag	2101	2125	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_J07004
gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagag
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgagga
gctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgag
ggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccca
ccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaa
gtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgag
gtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgg
ggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaa
cttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgac
ggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatc
acatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata