BBa_J07025
type: ComponentDefinition
persistent identity: BBa J07025
display id: BBa_J07025
version: 1
was generated by: iGEM to SBOL conversion
description: ToxR'::scFv Fusion 2 (with promoter and rbs and terminator)
created: 2005-07-13T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: see description for J07024. This scFv is somewhat stronger than the one used in J07024.
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 164
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: jenmitch
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0040
component: BBa_B0015
component: BBa_J07013
component: BBa_J07009
component: BBa_B0030
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_J07009
sequence annotation: BBa_J07013
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_J07025_sequence/1

BBa_R0040
type: Component
persistent identity: component2223673
display id: component2223673
version: 1
top level: BBa_J07025
definition: p(tetR)
access: public

BBa_B0015
type: Component
persistent identity: component2223704
display id: component2223704
version: 1
top level: BBa_J07025
definition: BBa_B0015
access: public

BBa_J07013
type: Component
persistent identity: component2223697
display id: component2223697
version: 1
top level: BBa_J07025
definition: BBa_J07013
access: public

BBa_J07009
type: Component
persistent identity: component2223686
display id: component2223686
version: 1
top level: BBa_J07025
definition: BBa_J07009
access: public

BBa_B0030
type: Component
persistent identity: component2223679
display id: component2223679
version: 1
top level: BBa_J07025
definition: BBa_B0030
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2223673
display id: annotation2223673
version: 1
top level: BBa_J07025
location: https://synbiohub.org/public/igem/BBa_J07025/annotation2223673/range2223673/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J07025/annotation2223673/range2223673/1
type: Range
persistent identity: range2223673
display id: range2223673
version: 1
top level: BBa_J07025
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2223679
display id: annotation2223679
version: 1
top level: BBa_J07025
location: https://synbiohub.org/public/igem/BBa_J07025/annotation2223679/range2223679/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J07025/annotation2223679/range2223679/1
type: Range
persistent identity: range2223679
display id: range2223679
version: 1
top level: BBa_J07025
direction: direction/forward
start: 63
end: 77
orientation: inline

BBa_J07009
type: SequenceAnnotation
persistent identity: annotation2223686
display id: annotation2223686
version: 1
top level: BBa_J07025
location: https://synbiohub.org/public/igem/BBa_J07025/annotation2223686/range2223686/1
component: BBa_J07009

https://synbiohub.org/public/igem/BBa_J07025/annotation2223686/range2223686/1
type: Range
persistent identity: range2223686
display id: range2223686
version: 1
top level: BBa_J07025
direction: direction/reverse
start: 84
end: 713
orientation: inline

BBa_J07013
type: SequenceAnnotation
persistent identity: annotation2223697
display id: annotation2223697
version: 1
top level: BBa_J07025
location: https://synbiohub.org/public/igem/BBa_J07025/annotation2223697/range2223697/1
component: BBa_J07013

https://synbiohub.org/public/igem/BBa_J07025/annotation2223697/range2223697/1
type: Range
persistent identity: range2223697
display id: range2223697
version: 1
top level: BBa_J07025
direction: direction/reverse
start: 722
end: 1483
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2223704
display id: annotation2223704
version: 1
top level: BBa_J07025
location: https://synbiohub.org/public/igem/BBa_J07025/annotation2223704/range2223704/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J07025/annotation2223704/range2223704/1
type: Range
persistent identity: range2223704
display id: range2223704
version: 1
top level: BBa_J07025
direction: direction/reverse
start: 1492
end: 1620
orientation: inline

BBa_J07013
type: ComponentDefinition
persistent identity: BBa J07013
display id: BBa_J07013
version: 1
was generated by: iGEM to SBOL conversion
description: scFv anti-fluorescein [S101A]
created: 2005-07-07T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: Ser H101 Ala the mid-strength of the 3 piece anti-flour escein scFv sequence set.
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: mit_igem_05
type: DnaRegion
role: SO:0000804
role: partType/Signalling
sequence annotation: VL
sequence annotation: CDR1 VL
sequence annotation: CDR2 VL
sequence annotation: CDR3 VL
sequence annotation: linker
sequence annotation: VH
sequence annotation: CDR1 VH
sequence annotation: CDR2 VH
sequence annotation: CDR3 VH
sequence annotation: S - A
sequence: https://synbiohub.org/public/igem/BBa_J07013_sequence/1

VL
type: SequenceAnnotation
persistent identity: annotation1582309
display id: annotation1582309
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582309/range1582309/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07013/annotation1582309/range1582309/1
type: Range
persistent identity: range1582309
display id: range1582309
version: 1
top level: BBa_J07013
direction: direction/forward
start: 1
end: 336
orientation: inline

CDR1 VL
type: SequenceAnnotation
persistent identity: annotation1582290
display id: annotation1582290
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582290/range1582290/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J07013/annotation1582290/range1582290/1
type: Range
persistent identity: range1582290
display id: range1582290
version: 1
top level: BBa_J07013
direction: direction/forward
start: 61
end: 120
orientation: inline

CDR2 VL
type: SequenceAnnotation
persistent identity: annotation1582296
display id: annotation1582296
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582296/range1582296/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J07013/annotation1582296/range1582296/1
type: Range
persistent identity: range1582296
display id: range1582296
version: 1
top level: BBa_J07013
direction: direction/forward
start: 163
end: 183
orientation: inline

CDR3 VL
type: SequenceAnnotation
persistent identity: annotation1582301
display id: annotation1582301
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582301/range1582301/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J07013/annotation1582301/range1582301/1
type: Range
persistent identity: range1582301
display id: range1582301
version: 1
top level: BBa_J07013
direction: direction/forward
start: 280
end: 306
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1582302
display id: annotation1582302
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582302/range1582302/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J07013/annotation1582302/range1582302/1
type: Range
persistent identity: range1582302
display id: range1582302
version: 1
top level: BBa_J07013
direction: direction/forward
start: 337
end: 411
orientation: inline

VH
type: SequenceAnnotation
persistent identity: annotation1582310
display id: annotation1582310
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582310/range1582310/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07013/annotation1582310/range1582310/1
type: Range
persistent identity: range1582310
display id: range1582310
version: 1
top level: BBa_J07013
direction: direction/forward
start: 412
end: 762
orientation: inline

CDR1 VH
type: SequenceAnnotation
persistent identity: annotation1582303
display id: annotation1582303
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582303/range1582303/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J07013/annotation1582303/range1582303/1
type: Range
persistent identity: range1582303
display id: range1582303
version: 1
top level: BBa_J07013
direction: direction/forward
start: 502
end: 513
orientation: inline

CDR2 VH
type: SequenceAnnotation
persistent identity: annotation1582304
display id: annotation1582304
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582304/range1582304/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07013/annotation1582304/range1582304/1
type: Range
persistent identity: range1582304
display id: range1582304
version: 1
top level: BBa_J07013
direction: direction/forward
start: 559
end: 615
orientation: inline

CDR3 VH
type: SequenceAnnotation
persistent identity: annotation1582308
display id: annotation1582308
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582308/range1582308/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07013/annotation1582308/range1582308/1
type: Range
persistent identity: range1582308
display id: range1582308
version: 1
top level: BBa_J07013
direction: direction/forward
start: 711
end: 732
orientation: inline

S - A
type: SequenceAnnotation
persistent identity: annotation1582289
display id: annotation1582289
version: 1
top level: BBa_J07013
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582289/range1582289/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J07013/annotation1582289/range1582289/1
type: Range
persistent identity: range1582289
display id: range1582289
version: 1
top level: BBa_J07013
direction: direction/forward
start: 712
end: 714
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J07009
type: ComponentDefinition
persistent identity: BBa J07009
display id: BBa_J07009
version: 1
was generated by: iGEM to SBOL conversion
description: toxicity-gene activator from Vibrio cholerae
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done @ aa 211. explanation of ToxR domains @ http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf ELIMINATE XbaI SITE PLZZZZZ
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: mit_igem_2005
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cytoplasmic Region
sequence annotation: Coding Sequence for ToxR'
sequence annotation: Start Codon
sequence annotation: Transmembrane Region
sequence annotation: Periplasmic Region
sequence: https://synbiohub.org/public/igem/BBa_J07009_sequence/1

Cytoplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668506
display id: annotation1668506
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
type: Range
persistent identity: range1668506
display id: range1668506
version: 1
top level: BBa_J07009
direction: direction/forward
start: 1
end: 546
orientation: inline

Coding Sequence for ToxR'
type: SequenceAnnotation
persistent identity: annotation1668507
display id: annotation1668507
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
type: Range
persistent identity: range1668507
display id: range1668507
version: 1
top level: BBa_J07009
direction: direction/forward
start: 1
end: 630
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1668508
display id: annotation1668508
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
type: Range
persistent identity: range1668508
display id: range1668508
version: 1
top level: BBa_J07009
direction: direction/forward
start: 6
end: 8
orientation: inline

Transmembrane Region
type: SequenceAnnotation
persistent identity: annotation1668509
display id: annotation1668509
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
type: Range
persistent identity: range1668509
display id: range1668509
version: 1
top level: BBa_J07009
direction: direction/forward
start: 547
end: 594
orientation: inline

Periplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668510
display id: annotation1668510
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
type: Range
persistent identity: range1668510
display id: range1668510
version: 1
top level: BBa_J07009
direction: direction/forward
start: 595
end: 630
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_J07025_sequence/1
type: Sequence
persistent identity: BBa J07025 sequence
display id: BBa_J07025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgtactagaggacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgattttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcagcagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtgacgtcaaactggatgagactggaggaggcttggtgcaacctgggaggcccatgaaactctcctgtgttgcctctggattcacttttagtgactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaaattagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacatgggtatctattactgtacgggtgcgtactatggtatggactactggggtcaaggaacctcagtcaccgtctcctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07013_sequence/1
type: Sequence
persistent identity: BBa J07013 sequence
display id: BBa_J07013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgattttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcagcagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtgacgtcaaactggatgagactggaggaggcttggtgcaacctgggaggcccatgaaactctcctgtgttgcctctggattcacttttagtgactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaaattagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacatgggtatctattactgtacgggtgcgtactatggtatggactactggggtcaaggaacctcagtcaccgtctcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07009_sequence/1
type: Sequence
persistent identity: BBa J07009 sequence
display id: BBa_J07009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z