LOCUS       BBa_J07026              1620 bp    DNA     linear       26-Dec-2024
DEFINITION  ToxR'::scFv Fusion 3  (with promoter and rbs and terminator)
ACCESSION   BBa_J07026
VERSION     BBa_J07026.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    84..629
                     /label=Cytoplasmic Region
     CDS             84..713
                     /label=Coding Sequence for ToxR'
     misc_feature    89..91
                     /label=Start Codon
     misc_feature    630..677
                     /label=Transmembrane Region
     misc_feature    678..713
                     /label=Periplasmic Region
     CDS             84..713
                     /label=BBa_J07009
     misc_feature    782..841
                     /label=CDR1 VL
     misc_feature    884..904
                     /label=CDR2 VL
     misc_feature    1001..1027
                     /label=CDR3 VL
     misc_feature    1058..1132
                     /label=linker
     misc_feature    1223..1234
                     /label=CDR1 VH
     misc_feature    1280..1336
                     /label=CDR2 VH
     misc_feature    1432..1453
                     /label=CDR3 VH
     misc_feature    722..1057
                     /label=VL
     misc_feature    1133..1483
                     /label=VH
     misc_feature    1433..1435
                     /label=S-A
     misc_feature    899..901
                     /label=F-V
     misc_feature    962..964
                     /label=S-N
     misc_feature    1133..1135
                     /label=D-G
     misc_feature    1178..1180
                     /label=R-G
     misc_feature    1181..1183
                     /label=P-A
     misc_feature    1202..1204
                     /label=A-T
     misc_feature    1220..1222
                     /label=S-G
     misc_feature    1223..1225
                     /label=D-H
     misc_feature    1283..1285
                     /label=I-F
     misc_feature    1409..1411
                     /label=M-T
     misc_feature    1433..1435
                     /label=S-A
     misc_feature    1436..1438
                     /label=Y-S
     misc_feature    1448..1450
                     /label=D-E
     misc_feature    1454..1456
                     /label=W-L
     misc_feature    722..1483
                     /label=BBa_J07014
     stem_loop       1503..1546
                     /label=stem_loop
     misc_feature    1492..1571
                     /label=BBa_B0010
     terminator      1492..1571
                     /label=BBa_B0010
     stem_loop       1587..1606
                     /label=T7 TE
     misc_feature    1613..1613
                     /label=stop
     polyA_site      1607..1620
                     /label=polya
     misc_feature    1580..1620
                     /label=BBa_B0012
     terminator      1580..1620
                     /label=BBa_B0012
     terminator      1492..1620
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgttcg gattaggaca caactcaaaa gagatatcga
      121 tgagtcatat tggtactaaa ttcattcttg ctgaaaaatt taccttcgat cccctaagca
      181 atactctgat tgacaaagaa gatagtgaag agatcattcg attaggcagc aacgaaagcc
      241 gaatcctttg gctgctggcc caacgtccaa acgaggtaat ttctcgcaat gatttgcatg
      301 actttgtttg gcgagagcaa ggttttgaag tcgatgattc cagcttaacc caagccattt
      361 cgactctgcg caaaatgctc aaagattcga caaagtcccc acaatacgtc aaaacggttc
      421 cgaagcgcgg ttaccaattg atcgcccgag tggaaacggt tgaagaagag atggctcgcg
      481 aaaacgaagc tgctcatgac atctctcagc cagaatctgt caatgaatac gcagaatcaa
      541 gcagtgtgcc ttcatcagcc actgtagtga acacaccgca gccagccaat gtcgtggcga
      601 ataaatcggc tccaaacttg gggaatcgac tgtttattct gatagcggtc ttacttcccc
      661 tcgcagtatt actgctcact aacccaagcc aatccagctt taaaccccta acgtactaga
      721 ggacgtcgtt atgactcaaa caccactatc acttcctgtt agtctaggtg atcaagcctc
      781 catctcttgc agatctagtc agagccttgt acacagtaat ggaaacacct atttacgttg
      841 gtacctgcaa aagccaggcc agtctccaaa ggtcctgatc tacaaagttt ccaaccgagt
      901 ttctggggtc ccagacaggt tcagtggcag tggatcaggg acagatttca cactcaagat
      961 caacagagtg gaggctgagg atctgggagt ttatttctgc tctcaaagta cacatgttcc
     1021 gtggacgttc ggtggaggca ccaagcttga aattaagtcc tctgctgatg atgctaagaa
     1081 ggatgctgct aagaaggatg atgctaagaa agatgatgct aagaaagatg gtggcgtcaa
     1141 actggatgag actggaggag gcttggtgca acctgggggg gccatgaaac tctcctgtgt
     1201 tacctctgga ttcacttttg gtcactactg gatgaactgg gtccgccagt ctccagagaa
     1261 aggactggag tgggtagcac aatttagaaa caaaccttat aattatgaaa catattattc
     1321 agattctgtg aaaggcagat tcaccatctc aagagatgat tccaaaagta gtgtctacct
     1381 gcaaatgaac aacttaagag ttgaagacac cggtatctat tactgtacgg gtgcgtctta
     1441 tggtatggaa tacttaggtc aaggaacctc agtcaccgtc tcctactaga gccaggcatc
     1501 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1561 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//