LOCUS       BBa_J07027              2274 bp    DNA     linear       17-May-2024
DEFINITION  ToxR'::PhoA (with promoter and RBS and terminator)
ACCESSION   BBa_J07027
VERSION     BBa_J07027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    84..629
                     /label=Cytoplasmic Region
     CDS             84..713
                     /label=Coding Sequence for ToxR'
     misc_feature    89..91
                     /label=Start Codon
     misc_feature    630..677
                     /label=Transmembrane Region
     misc_feature    678..713
                     /label=Periplasmic Region
     CDS             84..713
                     /label=BBa_J07009
     CDS             722..2137
                     /label=BBa_J07075
     stem_loop       2157..2200
                     /label=stem_loop
     misc_feature    2146..2225
                     /label=BBa_B0010
     terminator      2146..2225
                     /label=BBa_B0010
     stem_loop       2241..2260
                     /label=T7 TE
     misc_feature    2267..2267
                     /label=stop
     polyA_site      2261..2274
                     /label=polya
     misc_feature    2234..2274
                     /label=BBa_B0012
     terminator      2234..2274
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgttcg gattaggaca caactcaaaa gagatatcga
      121 tgagtcatat tggtactaaa ttcattcttg ctgaaaaatt taccttcgat cccctaagca
      181 atactctgat tgacaaagaa gatagtgaag agatcattcg attaggcagc aacgaaagcc
      241 gaatcctttg gctgctggcc caacgtccaa acgaggtaat ttctcgcaat gatttgcatg
      301 actttgtttg gcgagagcaa ggttttgaag tcgatgattc cagcttaacc caagccattt
      361 cgactctgcg caaaatgctc aaagattcga caaagtcccc acaatacgtc aaaacggttc
      421 cgaagcgcgg ttaccaattg atcgcccgag tggaaacggt tgaagaagag atggctcgcg
      481 aaaacgaagc tgctcatgac atctctcagc cagaatctgt caatgaatac gcagaatcaa
      541 gcagtgtgcc ttcatcagcc actgtagtga acacaccgca gccagccaat gtcgtggcga
      601 ataaatcggc tccaaacttg gggaatcgac tgtttattct gatagcggtc ttacttcccc
      661 tcgcagtatt actgctcact aacccaagcc aatccagctt taaaccccta acgtactaga
      721 ggtgaaacaa agcactattg cactggcact cttaccgtta ctgtttaccc ctgtgacaaa
      781 agcccggaca ccagaaatgc ctgttctgga aaaccgggct gctcagggcg atattactgc
      841 acccggcggt gctcgccgtt taacgggtga tcagactgcc gctctgcgtg attctcttag
      901 cgataaacct gcaaaaaata ttattttgct gattggcgat gggatggggg actcggaaat
      961 tactgccgca cgtaattatg ccgaaggtgc gggcggcttt tttaaaggta tagatgcctt
     1021 accgcttacc gggcaataca ctcactatgc gctgaataaa aaaaccggca aaccggacta
     1081 cgtcaccgac tcggctgcat cagcaaccgc ctggtcaacc ggtgtcaaaa cctataacgg
     1141 cgcgctgggc gtcgatattc acgaaaaaga tcacccaacg attctggaaa tggcaaaagc
     1201 cgcaggtctg gcgaccggta acgtttctac cgcagagttg caggatgcca cgcccgctgc
     1261 gctggtggca catgtgacct cgcgcaaatg ctacggtccg agcgcgacca gtgaaaaatg
     1321 tccgggtaac gctctggaaa aaggcggaaa aggatcgatt accgaacagc tgcttaacgc
     1381 tcgtgccgac gttacgcttg gcggcggcgc aaaaaccttt gctgaaacgg caaccgctgg
     1441 tgaatggcag ggaaaaacgc tgcgtgaaca ggcacaggcg cgtggttatc agttggtgag
     1501 cgatgctgcc tcactgaact cggtgacgga agcgaatcag caaaaacccc tgcttggcct
     1561 gtttgctgac ggcaatatgc cagtgcgctg gctaggaccg aaagcaacgt accatggcaa
     1621 tatcgataag cccgcagtca cctgtacgcc aaatccgcaa cgtaatgaca gtgtaccaac
     1681 cctggcgcag atgaccgaca aagccattga attgttgagt aaaaatgaga aaggcttttt
     1741 cctgcaagtt gaaggtgcgt caatcgataa acaggatcat gctgcgaatc cttgtgggca
     1801 aattggcgag acggtcgatc tcgatgaagc cgtacaacgg gcgctggagt tcgctaaaaa
     1861 ggagggtaac acgctggtca tagtcaccgc tgatcacgcc cacgccagcc agattgttgc
     1921 gccggatacc aaagctccgg gcctcaccca ggcgctaaat accaaagatg gcgcagtgat
     1981 ggtgatgagt tacgggaact ccgaagagga ttcacaagaa cataccggca gtcagttgcg
     2041 tattgcggcg tatggcccgc atgccgccaa tgttgttgga ctgaccgacc agaccgatct
     2101 cttctacacc atgaaagccg ctctggggct gaaataatac tagagccagg catcaaataa
     2161 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2221 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//