LOCUS       BBa_J07028              2047 bp    DNA     linear       22-Nov-2024
DEFINITION  ToxR'::MalE(with promoter and RBS and terminator)
ACCESSION   BBa_J07028
VERSION     BBa_J07028.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    84..629
                     /label=Cytoplasmic Region
     CDS             84..713
                     /label=Coding Sequence for ToxR'
     misc_feature    89..91
                     /label=Start Codon
     misc_feature    630..677
                     /label=Transmembrane Region
     misc_feature    678..713
                     /label=Periplasmic Region
     CDS             84..713
                     /label=BBa_J07009
     CDS             720..1910
                     /label=malE
     CDS             720..1910
                     /label=BBa_J07006
     stem_loop       1930..1973
                     /label=stem_loop
     misc_feature    1919..1998
                     /label=BBa_B0010
     terminator      1919..1998
                     /label=BBa_B0010
     stem_loop       2014..2033
                     /label=T7 TE
     misc_feature    2040..2040
                     /label=stop
     polyA_site      2034..2047
                     /label=polya
     misc_feature    2007..2047
                     /label=BBa_B0012
     terminator      2007..2047
                     /label=BBa_B0012
     terminator      1919..2047
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgttcg gattaggaca caactcaaaa gagatatcga
      121 tgagtcatat tggtactaaa ttcattcttg ctgaaaaatt taccttcgat cccctaagca
      181 atactctgat tgacaaagaa gatagtgaag agatcattcg attaggcagc aacgaaagcc
      241 gaatcctttg gctgctggcc caacgtccaa acgaggtaat ttctcgcaat gatttgcatg
      301 actttgtttg gcgagagcaa ggttttgaag tcgatgattc cagcttaacc caagccattt
      361 cgactctgcg caaaatgctc aaagattcga caaagtcccc acaatacgtc aaaacggttc
      421 cgaagcgcgg ttaccaattg atcgcccgag tggaaacggt tgaagaagag atggctcgcg
      481 aaaacgaagc tgctcatgac atctctcagc cagaatctgt caatgaatac gcagaatcaa
      541 gcagtgtgcc ttcatcagcc actgtagtga acacaccgca gccagccaat gtcgtggcga
      601 ataaatcggc tccaaacttg gggaatcgac tgtttattct gatagcggtc ttacttcccc
      661 tcgcagtatt actgctcact aacccaagcc aatccagctt taaaccccta acgtactaga
      721 tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt
      781 ccgcctcggc tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
      841 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
      901 tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg
      961 atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc
     1021 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg
     1081 atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc
     1141 tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
     1201 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
     1261 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
     1321 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
     1381 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
     1441 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
     1501 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
     1561 ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc
     1621 tggcgaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
     1681 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
     1741 cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
     1801 agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc
     1861 agactgtcga tgaagccctg aaagacgcgc agactcgtat caccaagtaa tactagagcc
     1921 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1981 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2041 gtttata
//