LOCUS       BBa_J07032              2626 bp    DNA     linear       22-Dec-2024
DEFINITION  FecI & FecR' test construct
ACCESSION   BBa_J07032
VERSION     BBa_J07032.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..757
                     /label=FecI
     CDS             230..757
                     /label=BBa_J07022
     misc_feature    1..757
                     /label=BBa_J07067
     RBS             766..780
                     /label=RBS-1\Strong
     RBS             773..777
                     /label=RBS
     misc_feature    766..780
                     /label=BBa_B0030
     RBS             766..780
                     /label=BBa_B0030
     CDS             787..1740
                     /label=FecR
     CDS             787..1740
                     /label=BBa_J07023
     misc_feature    766..1740
                     /label=BBa_J07071
     RBS             1749..1763
                     /label=RBS-1\Strong
     RBS             1756..1760
                     /label=RBS
     misc_feature    1749..1763
                     /label=BBa_B0030
     RBS             1749..1763
                     /label=BBa_B0030
     CDS             1770..2489
                     /label=GFP protein
     CDS             1770..2489
                     /label=BBa_E0040
     stem_loop       2509..2552
                     /label=stem_loop
     misc_feature    2498..2577
                     /label=BBa_B0010
     terminator      2498..2577
                     /label=BBa_B0010
     stem_loop       2593..2612
                     /label=T7 TE
     misc_feature    2619..2619
                     /label=stop
     polyA_site      2613..2626
                     /label=polya
     misc_feature    2586..2626
                     /label=BBa_B0012
     terminator      2586..2626
                     /label=BBa_B0012
     misc_feature    1749..2626
                     /label=BBa_E0840
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgtctgaccg
      241 cgccactacc acagcttcct taacgttcga gtcgctttat ggcacacatc acggctggtt
      301 gaaaagctgg ctgacgcgca aactccagtc tgcttttgat gcagatgaca ttgcccagga
      361 cacttttttg cgggtaatgg tcagcgaaac gctctcgacg atccgcgatc ctcgctcctt
      421 cctctgcact atcgccaaac gcgtgatggt ggacctgttt cgccgaaacg cgctggaaaa
      481 agcgtatctg gagatgctgg cgcttatgcc ggagggggga gcgccttcac ctgaggaacg
      541 cgaaagccaa ctcgagaccc tacaactcct cgacagcatg ctggacgggc taaacggcaa
      601 aacacgtgaa gcgtttctgc tttcgcaact ggatggtctg acatacagcg agattgcgca
      661 caaactcggt gtttccatca gctccgtgaa aaaatacgtg gcgaaagccg tcgagcactg
      721 cctgctgttc cgtctggagt atgggttatg ataataatac tagagattaa agaggagaaa
      781 tactagatga atcctttgtt aaccgattcc cgccgtcagg cgctgcgttc agcttcccac
      841 tggtatgccg tgctaagcgg cgagcgcgtc agcccacaac aggaagcgcg ctggcaacag
      901 tggtatgaac aggatcagga taaccagtgg gcctggcagc aggttgaaaa cctgcgcaac
      961 cagcttggcg gtgtgcctgg cgacgttgcc agccgggcgt tgcacgatac ccgcctcacc
     1021 cgccgtcacg tgatgaaagg attactgctg ttgctcggcg ctggcggagg ctggcagctc
     1081 tggcagtcgg aaaccggcga aggtctgcgg gcagattacc gcaccgccaa aggcacagtc
     1141 agccgtcagc aactggaaga tggctccctg ctcacgctga atacccaaag cgcggcggat
     1201 gtgcgttttg atgcgcatca gcgcaccgtc cggctctggt acggtgaaat cgccattact
     1261 accgcgaaag atgccctgca acgccccttc cgtgtcctga cccgtcaggg ccagctcact
     1321 gctttaggga cagaatttac cgtccgccag caggataatt tcacgcagct tgacgtgcag
     1381 cagcacgctg tggaagtgct tctcgccagt gcccccgcgc aaaaacgcat cgtgaacgct
     1441 ggtgaaagcc tacagttcag cgcctctgag tttggcgcag tgaaaccgct ggatgacgag
     1501 agtacaagct ggacgaagga catcctgagc ttcagcgata aaccgctggg tgaggtgata
     1561 gccacgctaa cccgttaccg caacggcgtg ctgcgctgcg atcccgccgt tgccgggctg
     1621 cgcctgagcg ggacgttccc gctgaaaaat accgatgcga tcctgaacgt tatcgcgcaa
     1681 acgcttcccg ttaaaattca gtctattacg cggtactgga taaacatttc accactgtaa
     1741 tactagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag
     1801 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1861 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1921 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     1981 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     2041 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     2101 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     2161 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     2221 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     2281 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     2341 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     2401 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     2461 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag
     2521 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     2581 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//