BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961222 1 BBa_R0010 range1961222 1 1 200 BBa_J07067 1 BBa_J07067 FecI test construct w/ inducible promoter 2005-08-21T11:00:00Z 2015-08-31T04:08:20Z Test construct for FecI Inducible promoter (regulated by LacI) + strong RBS (B0030) + FecI (J07022) false false _21_ 0 353 21 Not in stock false false Annie Vo component2219248 1 BBa_B0030 component2219240 1 BBa_R0010 component2219251 1 BBa_J07022 annotation2219251 1 BBa_J07022 range2219251 1 230 757 annotation2219248 1 BBa_B0030 range2219248 1 209 223 annotation2219240 1 BBa_R0010 range2219240 1 1 200 BBa_J07032 1 BBa_J07032 FecI & FecR' test construct 2005-07-18T11:00:00Z 2015-08-31T04:08:19Z This part contains a promoter regulated by LacI, an RBS, coding sequences of FecI and FecR, and terminator. false false _21_ 0 353 21 Not in stock false false Annie component2227057 1 BBa_E0840 component2227040 1 BBa_J07067 component2227046 1 BBa_J07071 annotation2227040 1 BBa_J07067 range2227040 1 1 757 annotation2227057 1 BBa_E0840 range2227057 1 1749 2626 annotation2227046 1 BBa_J07071 range2227046 1 766 1740 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_J07071 1 BBa_J07071 FecR' w/ strong RBS 2005-08-22T11:00:00Z 2015-08-31T04:08:20Z This part includes strong RBS (B0030), and FecR' (J07023). Intermediate for test construct. false false _21_ 0 353 21 Not in stock false false Annie Vo component1665552 1 BBa_B0030 component1665558 1 BBa_J07023 annotation1665558 1 BBa_J07023 range1665558 1 22 975 annotation1665552 1 BBa_B0030 range1665552 1 1 15 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_J07023 1 BBa_J07023 FecR' 2005-07-13T11:00:00Z 2015-08-31T04:08:19Z MG1655 Wildtype FecR with pst1 (@664) from: ctgcag to ctacag false false _21_ 0 384 21 It's complicated false true jxyu annotation1577975 1 FecR range1577975 1 1 954 BBa_J07022 1 BBa_J07022 sigma factor FecI controls iron transport 2005-07-12T11:00:00Z 2015-08-31T04:08:19Z MG1655 FecI Coding sequence false false _21_ 0 379 21 It's complicated false false ymk annotation1577017 1 FecI range1577017 1 1 528 BBa_E0840 1 GFP genera GFP generator 2004-10-17T11:00:00Z 2015-08-31T04:07:26Z Released HQ 2013 B0030.E0040.B0015 false true _11_1_ 0 61 7 In stock true true Jennifer Braff component1249242 1 BBa_E0040 component1249257 1 BBa_B0012 component1249247 1 BBa_B0010 component1249239 1 BBa_B0030 annotation1249257 1 BBa_B0012 range1249257 1 838 878 annotation1249239 1 BBa_B0030 range1249239 1 1 15 annotation1249242 1 BBa_E0040 range1249242 1 22 741 annotation1249247 1 BBa_B0010 range1249247 1 750 829 BBa_J07067_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataa BBa_J07071_sequence 1 attaaagaggagaaatactagatgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J07023_sequence 1 atgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_J07022_sequence 1 atgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataa BBa_J07032_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataatactagagattaaagaggagaaatactagatgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0030_sequence 1 attaaagaggagaaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0840_sequence 1 attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z