LOCUS       BBa_J07034              3211 bp    DNA     linear       22-Dec-2024
DEFINITION  FecA' :: GFP reporter
ACCESSION   BBa_J07034
VERSION     BBa_J07034.1  
FEATURES             Location/Qualifiers
     CDS             1..2325
                     /label=BBa_J07018
     RBS             2334..2348
                     /label=RBS-1\Strong
     RBS             2341..2345
                     /label=RBS
     misc_feature    2334..2348
                     /label=BBa_B0030
     RBS             2334..2348
                     /label=BBa_B0030
     CDS             2355..3074
                     /label=GFP protein
     CDS             2355..3074
                     /label=BBa_E0040
     stem_loop       3094..3137
                     /label=stem_loop
     misc_feature    3083..3162
                     /label=BBa_B0010
     terminator      3083..3162
                     /label=BBa_B0010
     stem_loop       3178..3197
                     /label=T7 TE
     misc_feature    3204..3204
                     /label=stop
     polyA_site      3198..3211
                     /label=polya
     misc_feature    3171..3211
                     /label=BBa_B0012
     terminator      3171..3211
                     /label=BBa_B0012
ORIGIN
        1 atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg ttaacaccat tcgcctgagc
       61 ctgctgccgc tggccggtct ctcgttttcc gcttttgctg cacaggttaa tatcgcaccg
      121 ggatcgctcg ataaagcgct caatcagtat gccgcacaca gcggatttac cctctcggtt
      181 gacgccagcc tgacgcgcgg caagcagagc aacggcctgc acggcgatta cgacgtcgag
      241 agcggcctgc aacaactgct ggacggcagc ggactgcaag taaaaccgct gggaaataac
      301 agctggacgc tggagcccgc gcccgcacca aaagaagatg ccctgaccgt ggtcggcgac
      361 tggctgggtg atgcgcgtga aaacgacgta tttgaacatg ctggcgcgcg tgacgtgatc
      421 cgccgtgagg atttcgccaa aaccggcgca accaccatgc gtgaggtact taaccgcatc
      481 cctggcgtca gcgcgccgga aaacaacggc accggcagcc acgacctggc gatgaacttt
      541 ggcatccggg gcctgaaccc gcgcctcgcc agccgctcga ccgtcctgat ggacggcatc
      601 cccgtcccct tcgcccctta cggtcagccg cagctttcac tggctcccgt ttcgctcggc
      661 aacatggatg ccattgacgt ggtacgcggt ggtggtgcgg tgcgttacgg accgcagagc
      721 gtgggcggcg tggtgaactt tgttacccgt gccattccgc aggactttgg tatcgaggcg
      781 ggcgtggaag gtcagctcag cccaacctct tcacaaaaca acccgaaaga gacgcacaac
      841 ctgatggtgg gcggcacagc ggacaacggt tttggcaccg cgctgctcta ctccggcacg
      901 cgcggcagtg actggcgcga gcacagcgcc acccgcatcg acgacctgat gctgaaaagc
      961 aaatatgcgc cggatgaggt gcacaccttc aacagcctgc tgcaatatta cgacggtgaa
     1021 gccgacatgc ccggtggcct gtctcgcgcg gattacgacg ccgatcgctg gcaatccacc
     1081 cgcccgtatg accgcttctg gggtcgtcgc aagctggcga gcctgggcta ccagttccag
     1141 ccagacagcc agcataaatt caacattcag gggttctaca cccaaaccct gcgcagcggc
     1201 tacctggagc aaggcaaacg catcaccctc tcgccgcgta actactgggt gcgcggtatt
     1261 gagccacgct acagccagat ctttatgatc ggcccttccg cgcacgaagt gggcgtgggc
     1321 tatcgctatt tgaatgaatc aacgcatgaa atgcgttact acaccgccac cagcagcggg
     1381 cagttgccgt ccggctcaag cccttacgac cgcgatacgc gttccggcac cgaggcgcac
     1441 gcctggtatc tggatgacaa aatcgacatc ggcaactgga ccatcacgcc gggtatgcgt
     1501 ttcgaacata tcgagtcata ccagaacaac gccatcacag gcacgcacga agaagtgagc
     1561 tataacgcac cgcttccggc gttgaacgtg ctctatcacc tgactgacag ctggaatctt
     1621 tatgcaaaca ctgaaggctc gttcggcacc gtacagtaca gccagattgg caaggctgtg
     1681 caaagcggca atgttgaacc ggaaaaagcg cgaacctggg aactcggtac ccgctacgac
     1741 gacggcgcgc tgacggcgga aatggggctg ttcctgatta actttaacaa tcagtacgac
     1801 tccaaccaga ccaacgacac cgtcactgca cgtggcaaaa cgcgccatac cgggctggaa
     1861 acgcaggcac gttacgatct gggtacgcta acgccaacgc ttgataacgt ttccatctac
     1921 gccagctatg cgtatgtgaa cgcggaaatc cgcgagaaag gcgacaccta cggcaatctg
     1981 gtaccattct ccccgaaaca taaaggcacg ctgggcgtgg actacaagcc aggaaactgg
     2041 acgttcaatc tgaacagcga tttccagtcc agccagtttg cggataacgc caatacggtg
     2101 aaagagagcg ccgacggcag taccggccgc attcccggct tcatgctctg gggcgcacgc
     2161 gtggcgtatg actttggccc gcagatggca gatctgaacc tggcattcgg tgtgaaaaac
     2221 atcttcgacc aggactactt catccgctct tatgacgaca acaacaaagg catctatgca
     2281 ggccagccgc gcacgctgta tatgcagggg tcgttgaagt tctgatacta gagattaaag
     2341 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     2401 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     2461 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2521 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
     2581 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     2641 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     2701 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     2761 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     2821 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
     2881 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     2941 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     3001 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     3061 tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     3121 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     3181 tcaccttcgg gtgggccttt ctgcgtttat a
//