LOCUS       BBa_J07035              3291 bp    DNA     linear       22-Dec-2024
DEFINITION  FecA'-GFP under inducible promoter and rbs
ACCESSION   BBa_J07035
VERSION     BBa_J07035.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..2405
                     /label=BBa_J07018
     misc_feature    1..2405
                     /label=BBa_J07033
     RBS             2414..2428
                     /label=RBS-1\Strong
     RBS             2421..2425
                     /label=RBS
     misc_feature    2414..2428
                     /label=BBa_B0030
     RBS             2414..2428
                     /label=BBa_B0030
     CDS             2435..3154
                     /label=GFP protein
     CDS             2435..3154
                     /label=BBa_E0040
     stem_loop       3174..3217
                     /label=stem_loop
     misc_feature    3163..3242
                     /label=BBa_B0010
     terminator      3163..3242
                     /label=BBa_B0010
     stem_loop       3258..3277
                     /label=T7 TE
     misc_feature    3284..3284
                     /label=stop
     polyA_site      3278..3291
                     /label=polya
     misc_feature    3251..3291
                     /label=BBa_B0012
     terminator      3251..3291
                     /label=BBa_B0012
     misc_feature    2414..3291
                     /label=BBa_E0840
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg
      121 ttaacaccat tcgcctgagc ctgctgccgc tggccggtct ctcgttttcc gcttttgctg
      181 cacaggttaa tatcgcaccg ggatcgctcg ataaagcgct caatcagtat gccgcacaca
      241 gcggatttac cctctcggtt gacgccagcc tgacgcgcgg caagcagagc aacggcctgc
      301 acggcgatta cgacgtcgag agcggcctgc aacaactgct ggacggcagc ggactgcaag
      361 taaaaccgct gggaaataac agctggacgc tggagcccgc gcccgcacca aaagaagatg
      421 ccctgaccgt ggtcggcgac tggctgggtg atgcgcgtga aaacgacgta tttgaacatg
      481 ctggcgcgcg tgacgtgatc cgccgtgagg atttcgccaa aaccggcgca accaccatgc
      541 gtgaggtact taaccgcatc cctggcgtca gcgcgccgga aaacaacggc accggcagcc
      601 acgacctggc gatgaacttt ggcatccggg gcctgaaccc gcgcctcgcc agccgctcga
      661 ccgtcctgat ggacggcatc cccgtcccct tcgcccctta cggtcagccg cagctttcac
      721 tggctcccgt ttcgctcggc aacatggatg ccattgacgt ggtacgcggt ggtggtgcgg
      781 tgcgttacgg accgcagagc gtgggcggcg tggtgaactt tgttacccgt gccattccgc
      841 aggactttgg tatcgaggcg ggcgtggaag gtcagctcag cccaacctct tcacaaaaca
      901 acccgaaaga gacgcacaac ctgatggtgg gcggcacagc ggacaacggt tttggcaccg
      961 cgctgctcta ctccggcacg cgcggcagtg actggcgcga gcacagcgcc acccgcatcg
     1021 acgacctgat gctgaaaagc aaatatgcgc cggatgaggt gcacaccttc aacagcctgc
     1081 tgcaatatta cgacggtgaa gccgacatgc ccggtggcct gtctcgcgcg gattacgacg
     1141 ccgatcgctg gcaatccacc cgcccgtatg accgcttctg gggtcgtcgc aagctggcga
     1201 gcctgggcta ccagttccag ccagacagcc agcataaatt caacattcag gggttctaca
     1261 cccaaaccct gcgcagcggc tacctggagc aaggcaaacg catcaccctc tcgccgcgta
     1321 actactgggt gcgcggtatt gagccacgct acagccagat ctttatgatc ggcccttccg
     1381 cgcacgaagt gggcgtgggc tatcgctatt tgaatgaatc aacgcatgaa atgcgttact
     1441 acaccgccac cagcagcggg cagttgccgt ccggctcaag cccttacgac cgcgatacgc
     1501 gttccggcac cgaggcgcac gcctggtatc tggatgacaa aatcgacatc ggcaactgga
     1561 ccatcacgcc gggtatgcgt ttcgaacata tcgagtcata ccagaacaac gccatcacag
     1621 gcacgcacga agaagtgagc tataacgcac cgcttccggc gttgaacgtg ctctatcacc
     1681 tgactgacag ctggaatctt tatgcaaaca ctgaaggctc gttcggcacc gtacagtaca
     1741 gccagattgg caaggctgtg caaagcggca atgttgaacc ggaaaaagcg cgaacctggg
     1801 aactcggtac ccgctacgac gacggcgcgc tgacggcgga aatggggctg ttcctgatta
     1861 actttaacaa tcagtacgac tccaaccaga ccaacgacac cgtcactgca cgtggcaaaa
     1921 cgcgccatac cgggctggaa acgcaggcac gttacgatct gggtacgcta acgccaacgc
     1981 ttgataacgt ttccatctac gccagctatg cgtatgtgaa cgcggaaatc cgcgagaaag
     2041 gcgacaccta cggcaatctg gtaccattct ccccgaaaca taaaggcacg ctgggcgtgg
     2101 actacaagcc aggaaactgg acgttcaatc tgaacagcga tttccagtcc agccagtttg
     2161 cggataacgc caatacggtg aaagagagcg ccgacggcag taccggccgc attcccggct
     2221 tcatgctctg gggcgcacgc gtggcgtatg actttggccc gcagatggca gatctgaacc
     2281 tggcattcgg tgtgaaaaac atcttcgacc aggactactt catccgctct tatgacgaca
     2341 acaacaaagg catctatgca ggccagccgc gcacgctgta tatgcagggg tcgttgaagt
     2401 tctgatacta gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac
     2461 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     2521 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     2581 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
     2641 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
     2701 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
     2761 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     2821 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     2881 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
     2941 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
     3001 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     3061 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     3121 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
     3181 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     3241 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//