LOCUS       BBa_J07036               977 bp    DNA     linear       29-Nov-2024
DEFINITION  Threshold: Repression module (cI repressor with junk region)
ACCESSION   BBa_J07036
VERSION     BBa_J07036.1  
FEATURES             Location/Qualifiers
     CDS             91..840
                     /label=cI
     misc_feature    91..93
                     /label=start
     CDS             94..163
                     /label=modified cI1 cds
     misc_feature    1..19
                     /label=TetR 1
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=TetR
     misc_feature    802..840
                     /label=SsrA
     misc_feature    835..840
                     /label=2
     misc_feature    55..74
                     /label=intentional junk dna
     misc_feature    55..55
                     /label=start
     RBS             77..82
                     /label=rbs
     misc_feature    85..88
                     /label=intentional junk dna
     misc_feature    55..162
                     /label=antisense RNA pairing
     misc_feature    1..840
                     /label=BBa_I1030
     misc_feature    1..840
                     /label=BBa_I1030
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacagcttt
       61 gcacaacagc aacgacagga aaccggttcg atgtcgacaa aaaagaaacc attaacacaa
      121 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt
      181 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct
      241 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc
      301 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa
      361 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt
      421 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg
      481 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc
      541 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac
      601 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt
      661 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag
      721 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag
      781 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttata
//