BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_J07012 1 BBa_J07012 scFv anti-fluorescein [4-4-20] 2005-07-07T11:00:00Z 2015-08-31T04:08:19Z <a href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WK7-4D4T62K-6&_coverDate=10%2F22%2F2004&_alid=294278690&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6899&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=8affbe01a7e9fdde11418e671760e144">Substantial Energetic Improvement with Minimal Structural Perturbation in a High Affinity Mutant Antibody </a> single chain Antibody fragment selected to bind to fluorescein molecules. This scFv is the weakest of the three specified sequences. DNA insert in the pCT-4-4-20 (also known as pcT-302) from NheI to XhoI false false _21_ 0 363 21 It's complicated false false mit_igem_2005 annotation1582131 1 VH range1582131 1 412 762 annotation1581982 1 CDR1 VL range1581982 1 61 120 annotation1582062 1 CDR2 VH range1582062 1 559 615 annotation1581987 1 CDR3 VL range1581987 1 280 306 annotation1582130 1 VL range1582130 1 1 336 annotation1582123 1 CDR3 VH range1582123 1 711 732 annotation1582003 1 Linker range1582003 1 337 411 annotation1582016 1 CDR1 VH range1582016 1 502 513 annotation1581986 1 CDR2 VL range1581986 1 163 183 BBa_J07043 1 BBa_J07043 J07012.B0015 (scFv 4-4-20.TT) 2005-08-02T11:00:00Z 2015-08-31T04:08:20Z Anti-fluorescein scFv (4-4-20, no mutations) with transcriptional terminator. false false _21_ 0 350 21 It's complicated false false Jenny Nguyen component1651139 1 BBa_B0012 component1651124 1 BBa_J07012 component1651129 1 BBa_B0010 annotation1651129 1 BBa_B0010 range1651129 1 771 850 annotation1651139 1 BBa_B0012 range1651139 1 859 899 annotation1651124 1 BBa_J07012 range1651124 1 1 762 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J07012_sequence 1 gacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgattttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcagcagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtgacgtcaaactggatgagactggaggaggcttggtgcaacctgggaggcccatgaaactctcctgtgttgcctctggattcacttttagtgactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaaattagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacatgggtatctattactgtacgggttcttactatggtatggactactggggtcaaggaacctcagtcaccgtctcc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J07043_sequence 1 gacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgattttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcagcagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtgacgtcaaactggatgagactggaggaggcttggtgcaacctgggaggcccatgaaactctcctgtgttgcctctggattcacttttagtgactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaaattagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacatgggtatctattactgtacgggttcttactatggtatggactactggggtcaaggaacctcagtcaccgtctcctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z