LOCUS       BBa_J07048              3147 bp    DNA     linear       22-Dec-2024
DEFINITION  FecA::scFv internal fusion type 1 @ aa155
ACCESSION   BBa_J07048
VERSION     BBa_J07048.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=EcoRI & XbaI
     CDS             21..485
                     /label=FecA aa 1-155
     CDS             486..1247
                     /label=scFv
     misc_feature    1248..1304
                     /label=external linker
     CDS             1305..3164
                     /label=FecA aa 156-755
     misc_feature    3165..3185
                     /label=SpeI & PstI
ORIGIN
        1 atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg ttaacaccat tcgcctgagc
       61 ctgctgccgc tggccggtct ctcgttttcc gcttttgctg cacaggttaa tatcgcaccg
      121 ggatcgctcg ataaagcgct caatcagtat gccgcacaca gcggatttac cctctcggtt
      181 gacgccagcc tgacgcgcgg caagcagagc aacggcctgc acggcgatta cgacgtcgag
      241 agcggcctgc aacaactgct ggacggcagc ggactgcaag taaaaccgct gggaaataac
      301 agctggacgc tggagcccgc gcccgcacca aaagaagatg ccctgaccgt ggtcggcgac
      361 tggctgggtg atgcgcgtga aaacgacgta tttgaacatg ctggcgcgcg tgacgtgatc
      421 cgccgtgagg atttcgccaa aaccggcgca accaccatgc gtgaggacgt cgttatgact
      481 caaacaccac tatcacttcc tgttagtcta ggtgatcaag cctccatctc ttgcagatct
      541 agtcagagcc ttgtacacag taatggaaac acctatttac gttggtacct gcaaaagcca
      601 ggccagtctc caaaggtcct gatctacaaa gtttccaacc gagtttctgg ggtcccagac
      661 aggttcagtg gcagtggatc agggacagat ttcacactca agatcaacag agtggaggct
      721 gaggatctgg gagtttattt ctgctctcaa agtacacatg ttccgtggac gttcggtgga
      781 ggcaccaagc ttgaaattaa gtcctctgct gatgatgcta agaaggatgc tgctaagaag
      841 gatgatgcta agaaagatga tgctaagaaa gatggtggcg tcaaactgga tgagactgga
      901 ggaggcttgg tgcaacctgg gggggccatg aaactctcct gtgttacctc tggattcact
      961 tttggtcact actggatgaa ctgggtccgc cagtctccag agaaaggact ggagtgggta
     1021 gcacaattta gaaacaaacc ttataattat gaaacatatt attcagattc tgtgaaaggc
     1081 agattcacca tctcaagaga tgattccaaa agtagtgtct acctgcaaat gaacaactta
     1141 agagttgaag acaccggtat ctattactgt acgggtgcgt cttatggtat ggaatactta
     1201 ggtcaaggaa cctcagtcac cgtctccccg aacagcgcga gccatagcgg cagcgcgccg
     1261 aacaccagca gcgcgccggg cagcgtactt aaccgcatcc ctggcgtcag cgcgccggaa
     1321 aacaacggca ccggcagcca cgacctggcg atgaactttg gcatccgggg cctgaacccg
     1381 cgcctcgcca gccgctcgac cgtcctgatg gacggcatcc ccgtcccctt cgccccttac
     1441 ggtcagccgc agctttcact ggctcccgtt tcgctcggca acatggatgc cattgacgtg
     1501 gtacgcggtg gtggtgcggt gcgttacgga ccgcagagcg tgggcggcgt ggtgaacttt
     1561 gttacccgtg ccattccgca ggactttggt atcgaggcgg gcgtggaagg tcagctcagc
     1621 ccaacctctt cacaaaacaa cccgaaagag acgcacaacc tgatggtggg cggcacagcg
     1681 gacaacggtt ttggcaccgc gctgctctac tccggcacgc gcggcagtga ctggcgcgag
     1741 cacagcgcca cccgcatcga cgacctgatg ctgaaaagca aatatgcgcc ggatgaggtg
     1801 cacaccttca acagcctgct gcaatattac gacggtgaag ccgacatgcc cggtggcctg
     1861 tctcgcgcgg attacgacgc cgatcgctgg caatccaccc gcccgtatga ccgcttctgg
     1921 ggtcgtcgca agctggcgag cctgggctac cagttccagc cagacagcca gcataaattc
     1981 aacattcagg ggttctacac ccaaaccctg cgcagcggct acctggagca aggcaaacgc
     2041 atcaccctct cgccgcgtaa ctactgggtg cgcggtattg agccacgcta cagccagatc
     2101 tttatgatcg gcccttccgc gcacgaagtg ggcgtgggct atcgctattt gaatgaatca
     2161 acgcatgaaa tgcgttacta caccgccacc agcagcgggc agttgccgtc cggctcaagc
     2221 ccttacgacc gcgatacgcg ttccggcacc gaggcgcacg cctggtatct ggatgacaaa
     2281 atcgacatcg gcaactggac catcacgccg ggtatgcgtt tcgaacatat cgagtcatac
     2341 cagaacaacg ccatcacagg cacgcacgaa gaagtgagct ataacgcacc gcttccggcg
     2401 ttgaacgtgc tctatcacct gactgacagc tggaatcttt atgcaaacac tgaaggctcg
     2461 ttcggcaccg tacagtacag ccagattggc aaggctgtgc aaagcggcaa tgttgaaccg
     2521 gaaaaagcgc gaacctggga actcggtacc cgctacgacg acggcgcgct gacggcggaa
     2581 atggggctgt tcctgattaa ctttaacaat cagtacgact ccaaccagac caacgacacc
     2641 gtcactgcac gtggcaaaac gcgccatacc gggctggaaa cgcaggcacg ttacgatctg
     2701 ggtacgctaa cgccaacgct tgataacgtt tccatctacg ccagctatgc gtatgtgaac
     2761 gcggaaatcc gcgagaaagg cgacacctac ggcaatctgg taccattctc cccgaaacat
     2821 aaaggcacgc tgggcgtgga ctacaagcca ggaaactgga cgttcaatct gaacagcgat
     2881 ttccagtcca gccagtttgc ggataacgcc aatacggtga aagagagcgc cgacggcagt
     2941 accggccgca ttcccggctt catgctctgg ggcgcacgcg tggcgtatga ctttggcccg
     3001 cagatggcag atctgaacct ggcattcggt gtgaaaaaca tcttcgacca ggactacttc
     3061 atccgctctt atgacgacaa caacaaaggc atctatgcag gccagccgcg cacgctgtat
     3121 atgcaggggt cgttgaagtt ctaataa
//