LOCUS       BBa_J07050              3144 bp    DNA     linear       27-Mar-2025
DEFINITION  FecA::scFv internal fusion type 1 @ aa178
ACCESSION   BBa_J07050
VERSION     BBa_J07050.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=EcoRI & XbaI
     CDS             21..554
                     /label=FecA aa 1-178
     CDS             555..1316
                     /label=scFv
     misc_feature    1317..1373
                     /label=linker
     CDS             1374..3164
                     /label=FecA aa 178-755
     misc_feature    3165..3185
                     /label=SpeI & PstI
ORIGIN
        1 atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg ttaacaccat tcgcctgagc
       61 ctgctgccgc tggccggtct ctcgttttcc gcttttgctg cacaggttaa tatcgcaccg
      121 ggatcgctcg ataaagcgct caatcagtat gccgcacaca gcggatttac cctctcggtt
      181 gacgccagcc tgacgcgcgg caagcagagc aacggcctgc acggcgatta cgacgtcgag
      241 agcggcctgc aacaactgct ggacggcagc ggactgcaag taaaaccgct gggaaataac
      301 agctggacgc tggagcccgc gcccgcacca aaagaagatg ccctgaccgt ggtcggcgac
      361 tggctgggtg atgcgcgtga aaacgacgta tttgaacatg ctggcgcgcg tgacgtgatc
      421 cgccgtgagg atttcgccaa aaccggcgca accaccatgc gtgaggtact taaccgcatc
      481 cctggcgtca gcgcgccgga aaacaacggc accggcagcc acgacctggc gatggacgtc
      541 gttatgactc aaacaccact atcacttcct gttagtctag gtgatcaagc ctccatctct
      601 tgcagatcta gtcagagcct tgtacacagt aatggaaaca cctatttacg ttggtacctg
      661 caaaagccag gccagtctcc aaaggtcctg atctacaaag tttccaaccg agtttctggg
      721 gtcccagaca ggttcagtgg cagtggatca gggacagatt tcacactcaa gatcaacaga
      781 gtggaggctg aggatctggg agtttatttc tgctctcaaa gtacacatgt tccgtggacg
      841 ttcggtggag gcaccaagct tgaaattaag tcctctgctg atgatgctaa gaaggatgct
      901 gctaagaagg atgatgctaa gaaagatgat gctaagaaag atggtggcgt caaactggat
      961 gagactggag gaggcttggt gcaacctggg ggggccatga aactctcctg tgttacctct
     1021 ggattcactt ttggtcacta ctggatgaac tgggtccgcc agtctccaga gaaaggactg
     1081 gagtgggtag cacaatttag aaacaaacct tataattatg aaacatatta ttcagattct
     1141 gtgaaaggca gattcaccat ctcaagagat gattccaaaa gtagtgtcta cctgcaaatg
     1201 aacaacttaa gagttgaaga caccggtatc tattactgta cgggtgcgtc ttatggtatg
     1261 gaatacttag gtcaaggaac ctcagtcacc gtctccccga acagcgcgag ccatagcggc
     1321 agcgcgccga acaccagcag cgcgccgggc agcaactttg gcatccgggg cctgaacccg
     1381 cgcctcgcca gccgctcgac cgtcctgatg gacggcatcc ccgtcccctt cgccccttac
     1441 ggtcagccgc agctttcact ggctcccgtt tcgctcggca acatggatgc cattgacgtg
     1501 gtacgcggtg gtggtgcggt gcgttacgga ccgcagagcg tgggcggcgt ggtgaacttt
     1561 gttacccgtg ccattccgca ggactttggt atcgaggcgg gcgtggaagg tcagctcagc
     1621 ccaacctctt cacaaaacaa cccgaaagag acgcacaacc tgatggtggg cggcacagcg
     1681 gacaacggtt ttggcaccgc gctgctctac tccggcacgc gcggcagtga ctggcgcgag
     1741 cacagcgcca cccgcatcga cgacctgatg ctgaaaagca aatatgcgcc ggatgaggtg
     1801 cacaccttca acagcctgct gcaatattac gacggtgaag ccgacatgcc cggtggcctg
     1861 tctcgcgcgg attacgacgc cgatcgctgg caatccaccc gcccgtatga ccgcttctgg
     1921 ggtcgtcgca agctggcgag cctgggctac cagttccagc cagacagcca gcataaattc
     1981 aacattcagg ggttctacac ccaaaccctg cgcagcggct acctggagca aggcaaacgc
     2041 atcaccctct cgccgcgtaa ctactgggtg cgcggtattg agccacgcta cagccagatc
     2101 tttatgatcg gcccttccgc gcacgaagtg ggcgtgggct atcgctattt gaatgaatca
     2161 acgcatgaaa tgcgttacta caccgccacc agcagcgggc agttgccgtc cggctcaagc
     2221 ccttacgacc gcgatacgcg ttccggcacc gaggcgcacg cctggtatct ggatgacaaa
     2281 atcgacatcg gcaactggac catcacgccg ggtatgcgtt tcgaacatat cgagtcatac
     2341 cagaacaacg ccatcacagg cacgcacgaa gaagtgagct ataacgcacc gcttccggcg
     2401 ttgaacgtgc tctatcacct gactgacagc tggaatcttt atgcaaacac tgaaggctcg
     2461 ttcggcaccg tacagtacag ccagattggc aaggctgtgc aaagcggcaa tgttgaaccg
     2521 gaaaaagcgc gaacctggga actcggtacc cgctacgacg acggcgcgct gacggcggaa
     2581 atggggctgt tcctgattaa ctttaacaat cagtacgact ccaaccagac caacgacacc
     2641 gtcactgcac gtggcaaaac gcgccatacc gggctggaaa cgcaggcacg ttacgatctg
     2701 ggtacgctaa cgccaacgct tgataacgtt tccatctacg ccagctatgc gtatgtgaac
     2761 gcggaaatcc gcgagaaagg cgacacctac ggcaatctgg taccattctc cccgaaacat
     2821 aaaggcacgc tgggcgtgga ctacaagcca ggaaactgga cgttcaatct gaacagcgat
     2881 ttccagtcca gccagtttgc ggataacgcc aatacggtga aagagagcgc cgacggcagt
     2941 accggccgca ttcccggctt catgctctgg ggcgcacgcg tggcgtatga ctttggcccg
     3001 cagatggcag atctgaacct ggcattcggt gtgaaaaaca tcttcgacca ggactacttc
     3061 atccgctctt atgacgacaa caacaaaggc atctatgcag gccagccgcg cacgctgtat
     3121 atgcaggggt cgttgaagtt ctga
//