LOCUS       BBa_J07070              2069 bp    DNA     linear       25-Nov-2024
DEFINITION  FecR' test construct w/ inducible promoter
ACCESSION   BBa_J07070
VERSION     BBa_J07070.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..1183
                     /label=FecR
     CDS             230..1183
                     /label=BBa_J07023
     RBS             1192..1206
                     /label=RBS-1\Strong
     RBS             1199..1203
                     /label=RBS
     misc_feature    1192..1206
                     /label=BBa_B0030
     RBS             1192..1206
                     /label=BBa_B0030
     CDS             1213..1932
                     /label=GFP protein
     CDS             1213..1932
                     /label=BBa_E0040
     stem_loop       1952..1995
                     /label=stem_loop
     misc_feature    1941..2020
                     /label=BBa_B0010
     terminator      1941..2020
                     /label=BBa_B0010
     stem_loop       2036..2055
                     /label=T7 TE
     misc_feature    2062..2062
                     /label=stop
     polyA_site      2056..2069
                     /label=polya
     misc_feature    2029..2069
                     /label=BBa_B0012
     terminator      2029..2069
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgaatccttt
      241 gttaaccgat tcccgccgtc aggcgctgcg ttcagcttcc cactggtatg ccgtgctaag
      301 cggcgagcgc gtcagcccac aacaggaagc gcgctggcaa cagtggtatg aacaggatca
      361 ggataaccag tgggcctggc agcaggttga aaacctgcgc aaccagcttg gcggtgtgcc
      421 tggcgacgtt gccagccggg cgttgcacga tacccgcctc acccgccgtc acgtgatgaa
      481 aggattactg ctgttgctcg gcgctggcgg aggctggcag ctctggcagt cggaaaccgg
      541 cgaaggtctg cgggcagatt accgcaccgc caaaggcaca gtcagccgtc agcaactgga
      601 agatggctcc ctgctcacgc tgaataccca aagcgcggcg gatgtgcgtt ttgatgcgca
      661 tcagcgcacc gtccggctct ggtacggtga aatcgccatt actaccgcga aagatgccct
      721 gcaacgcccc ttccgtgtcc tgacccgtca gggccagctc actgctttag ggacagaatt
      781 taccgtccgc cagcaggata atttcacgca gcttgacgtg cagcagcacg ctgtggaagt
      841 gcttctcgcc agtgcccccg cgcaaaaacg catcgtgaac gctggtgaaa gcctacagtt
      901 cagcgcctct gagtttggcg cagtgaaacc gctggatgac gagagtacaa gctggacgaa
      961 ggacatcctg agcttcagcg ataaaccgct gggtgaggtg atagccacgc taacccgtta
     1021 ccgcaacggc gtgctgcgct gcgatcccgc cgttgccggg ctgcgcctga gcgggacgtt
     1081 cccgctgaaa aataccgatg cgatcctgaa cgttatcgcg caaacgcttc ccgttaaaat
     1141 tcagtctatt acgcggtact ggataaacat ttcaccactg taatactaga gattaaagag
     1201 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1261 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1321 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1381 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1441 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1501 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1561 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1621 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1681 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1741 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1801 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1861 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1921 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1981 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2041 accttcgggt gggcctttct gcgtttata
//