LOCUS       BBa_J07076              2269 bp    DNA     linear       22-Dec-2024
DEFINITION  promotor, rbs, toxr'-phoa, terminator
ACCESSION   BBa_J07076
VERSION     BBa_J07076.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..626
                     /label=Cytoplasmic Region
     CDS             81..710
                     /label=Coding Sequence for ToxR'
     misc_feature    86..88
                     /label=Start Codon
     misc_feature    627..674
                     /label=Transmembrane Region
     misc_feature    675..710
                     /label=Periplasmic Region
     CDS             81..710
                     /label=BBa_J07009
     CDS             717..2132
                     /label=PhoA
     misc_feature    717..2132
                     /label=BBa_J07005
     stem_loop       2152..2195
                     /label=stem_loop
     misc_feature    2141..2220
                     /label=BBa_B0010
     terminator      2141..2220
                     /label=BBa_B0010
     stem_loop       2236..2255
                     /label=T7 TE
     misc_feature    2262..2262
                     /label=stop
     polyA_site      2256..2269
                     /label=polya
     misc_feature    2229..2269
                     /label=BBa_B0012
     terminator      2229..2269
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgttcggat taggacacaa ctcaaaagag atatcgatga
      121 gtcatattgg tactaaattc attcttgctg aaaaatttac cttcgatccc ctaagcaata
      181 ctctgattga caaagaagat agtgaagaga tcattcgatt aggcagcaac gaaagccgaa
      241 tcctttggct gctggcccaa cgtccaaacg aggtaatttc tcgcaatgat ttgcatgact
      301 ttgtttggcg agagcaaggt tttgaagtcg atgattccag cttaacccaa gccatttcga
      361 ctctgcgcaa aatgctcaaa gattcgacaa agtccccaca atacgtcaaa acggttccga
      421 agcgcggtta ccaattgatc gcccgagtgg aaacggttga agaagagatg gctcgcgaaa
      481 acgaagctgc tcatgacatc tctcagccag aatctgtcaa tgaatacgca gaatcaagca
      541 gtgtgccttc atcagccact gtagtgaaca caccgcagcc agccaatgtc gtggcgaata
      601 aatcggctcc aaacttgggg aatcgactgt ttattctgat agcggtctta cttcccctcg
      661 cagtattact gctcactaac ccaagccaat ccagctttaa acccctaacg tactagatga
      721 aacagagcac cattgcgctg gcgctgctgc cgctgctgtt taccccggtg accaaagcgc
      781 gcaccccgga aatgccggtg ctggaaaacc gcgcggcgca gggcgatatt accgcgccgg
      841 gcggcgcgcg ccgcctgacc ggcgatcaga ccgcggcgct gcgcgatagc ctgagcgata
      901 aaccggcgaa aaacattatt ctgctgattg gcgatggcat gggcgatagc gaaattaccg
      961 cggcgcgcaa ctatgcggaa ggcgcgggcg gcttttttaa aggcattgat gcgctgccgc
     1021 tgaccggcca gtatacccat tatgcgctga acaaaaaaac cggcaaaccg gattatgtga
     1081 ccgatagcgc ggcgagcgcg accgcgtgga gcaccggcgt gaaaacctat aacggcgcgc
     1141 tgggcgtgga tattcatgaa aaagatcatc cgaccattct ggaaatggcg aaagcggcgg
     1201 gcctggcgac cggcaacgtg agcaccgcgg aactgcaaga tgcgaccccg gcggcgctgg
     1261 tggcgcatgt gaccagccgc aaatgctatg gcccgagcgc gaccagcgaa aaatgcccgg
     1321 gcaacgcgct ggaaaaaggc ggcaaaggca gcattaccga acagctgctg aacgcgcgcg
     1381 cggatgtgac cctgggcggc ggcgcgaaaa cctttgcgga aaccgcgacc gcgggcgaat
     1441 ggcagggcaa aaccctgcgc gaacaggcgc aggcgcgcgg ctatcagctg gtgagcgatg
     1501 cggcgagcct gaacagcgtg accgaagcga accagcagaa accgctgctg ggcctgtttg
     1561 cggatggcaa catgccggtg cgctggctgg gcccgaaagc gacctatcat ggcaacattg
     1621 ataaaccggc ggtgacctgc accccgaacc cgcagcgcaa cgatagcgtg ccgaccctgg
     1681 cgcagatgac cgataaagcg attgaactgc tgagcaaaaa cgaaaaaggc ttttttctgc
     1741 aagtggaagg cgcgagcatt gataaacagg atcatgcggc gaacccgtgc ggccagattg
     1801 gcgaaaccgt ggatctggat gaagcggtgc agcgcgcgct ggaatttgcg aaaaaagaag
     1861 gcaacaccct ggtgattgtg accgcggatc atgcgcatgc gagccagatt gtggcgccgg
     1921 ataccaaagc gccgggcctg acccaggcgc tgaacaccaa agatggcgcg gtgatggtga
     1981 tgagctatgg caacagcgaa gaagatagcc aggaacatac cggcagccag ctgcgcattg
     2041 cggcgtatgg cccgcatgcg gcgaacgtgg tgggcctgac cgatcagacc gatctgtttt
     2101 ataccatgaa agcggcgctg ggcctgaaat aatactagag ccaggcatca aataaaacga
     2161 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2221 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//