LOCUS       BBa_J07077              2044 bp    DNA     linear       26-Dec-2024
DEFINITION  promotor rbs ToxR MalE terminator
ACCESSION   BBa_J07077
VERSION     BBa_J07077.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..626
                     /label=Cytoplasmic Region
     CDS             81..710
                     /label=Coding Sequence for ToxR'
     misc_feature    86..88
                     /label=Start Codon
     misc_feature    627..674
                     /label=Transmembrane Region
     misc_feature    675..710
                     /label=Periplasmic Region
     CDS             81..710
                     /label=BBa_J07009
     CDS             717..1907
                     /label=malE
     CDS             717..1907
                     /label=BBa_J07006
     stem_loop       1927..1970
                     /label=stem_loop
     misc_feature    1916..1995
                     /label=BBa_B0010
     terminator      1916..1995
                     /label=BBa_B0010
     stem_loop       2011..2030
                     /label=T7 TE
     misc_feature    2037..2037
                     /label=stop
     polyA_site      2031..2044
                     /label=polya
     misc_feature    2004..2044
                     /label=BBa_B0012
     terminator      2004..2044
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgttcggat taggacacaa ctcaaaagag atatcgatga
      121 gtcatattgg tactaaattc attcttgctg aaaaatttac cttcgatccc ctaagcaata
      181 ctctgattga caaagaagat agtgaagaga tcattcgatt aggcagcaac gaaagccgaa
      241 tcctttggct gctggcccaa cgtccaaacg aggtaatttc tcgcaatgat ttgcatgact
      301 ttgtttggcg agagcaaggt tttgaagtcg atgattccag cttaacccaa gccatttcga
      361 ctctgcgcaa aatgctcaaa gattcgacaa agtccccaca atacgtcaaa acggttccga
      421 agcgcggtta ccaattgatc gcccgagtgg aaacggttga agaagagatg gctcgcgaaa
      481 acgaagctgc tcatgacatc tctcagccag aatctgtcaa tgaatacgca gaatcaagca
      541 gtgtgccttc atcagccact gtagtgaaca caccgcagcc agccaatgtc gtggcgaata
      601 aatcggctcc aaacttgggg aatcgactgt ttattctgat agcggtctta cttcccctcg
      661 cagtattact gctcactaac ccaagccaat ccagctttaa acccctaacg tactagatga
      721 aaataaaaac aggtgcacgc atcctcgcat tatccgcatt aacgacgatg atgttttccg
      781 cctcggctct cgccaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag
      841 gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca
      901 ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg
      961 gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt
     1021 tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg
     1081 ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga
     1141 tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg
     1201 ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact
     1261 tcacctggcc gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt
     1321 acgacattaa agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg
     1381 ttgacctgat taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg
     1441 cctttaataa aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg tccaacatcg
     1501 acaccagcaa agtgaattat ggtgtaacgg tactgccgac cttcaagggt caaccatcca
     1561 aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac aaagagctgg
     1621 cgaaagagtt cctcgaaaac tatctgctga ctgatgaagg tctggaagcg gttaataaag
     1681 acaaaccgct gggtgccgta gcgctgaagt cttacgagga agagttggcg aaagatccac
     1741 gtattgccgc caccatggaa aacgcccaga aaggtgaaat catgccgaac atcccgcaga
     1801 tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga
     1861 ctgtcgatga agccctgaaa gacgcgcaga ctcgtatcac caagtaatac tagagccagg
     1921 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1981 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2041 tata
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