##gff-version 3
##sequence-region BBa_J07077 1 2044
BBa_J07077	.	terminator	2004	2044	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J07077	.	stem_loop	2011	2030	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07077	.	polyA_site	2031	2044	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07077	.	stop_codon	2037	2037	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07077	.	engineered_region	2004	2044	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07077	.	CDS	81	710	.	+	0	ID=BBa_J07009;Name=BBa_J07009
BBa_J07077	.	sequence_feature	675	710	.	+	0	ID=annotation1668510;Name=Periplasmic Region;Parent=BBa_J07009
BBa_J07077	.	sequence_feature	81	626	.	+	0	ID=annotation1668506;Name=Cytoplasmic Region;Parent=BBa_J07009
BBa_J07077	.	CDS	81	710	.	+	0	ID=annotation1668507;Name=Coding Sequence for ToxR';Parent=BBa_J07009
BBa_J07077	.	sequence_feature	627	674	.	+	0	ID=annotation1668509;Name=Transmembrane Region;Parent=BBa_J07009
BBa_J07077	.	start_codon	86	88	.	+	0	ID=annotation1668508;Name=Start Codon;Parent=BBa_J07009
BBa_J07077	.	terminator	1916	1995	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J07077	.	stem_loop	1927	1970	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07077	.	engineered_region	1916	1995	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07077	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J07077	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07077	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J07077	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07077	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07077	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07077	.	CDS	717	1907	.	+	0	ID=BBa_J07006;Name=BBa_J07006
BBa_J07077	.	CDS	717	1907	.	+	0	ID=annotation1569704;Name=malE;Parent=BBa_J07006
BBa_J07077	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J07077	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J07077
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaattt
accttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagcc
gaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagca
aggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtcc
ccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctc
gcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatc
agccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgttt
attctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgt
actagatgaaaataaaaacaggtgcacgcatcctcgcattatccgcattaacgacgatgatgttttccgcctcggctct
cgccaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaag
aaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcgg
caactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctga
aatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgatt
gcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaag
agatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcac
ctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtg
gataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgatt
actccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcga
caccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctg
agcgcaggtattaacgccgccagtccgaacaaagagctggcgaaagagttcctcgaaaactatctgctgactgatgaag
gtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatcc
acgtattgccgccaccatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtat
gccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactcgta
tcaccaagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata