ToxR-MalE
type: ComponentDefinition
persistent identity: BBa J07077
display id: BBa_J07077
version: 1
was generated by: iGEM to SBOL conversion
description: promotor rbs ToxR MalE terminator
created: 2005-08-26T11:00:00Z
modified: 2015-08-31T04:08:20Z
owned by: james
owned by: myers
mutable description: promotor rbs ToxR MalE terminator
discontinued: true
dominant: false
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Will Bosworth
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_J07006
component: BBa_J07009
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_J07009
sequence annotation: BBa_J07006
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J07077_sequence/1

BBa_B0012
type: Component
persistent identity: component1672995
display id: component1672995
version: 1
top level: ToxR-MalE
definition: BBa_B0012
access: public

BBa_R0040
type: Component
persistent identity: component1672950
display id: component1672950
version: 1
top level: ToxR-MalE
definition: p(tetR)
access: public

BBa_B0010
type: Component
persistent identity: component1672985
display id: component1672985
version: 1
top level: ToxR-MalE
definition: BBa_B0010
access: public

BBa_J07006
type: Component
persistent identity: component1672980
display id: component1672980
version: 1
top level: ToxR-MalE
definition: BBa_J07006
access: public

BBa_J07009
type: Component
persistent identity: component1672975
display id: component1672975
version: 1
top level: ToxR-MalE
definition: BBa_J07009
access: public

BBa_B0034
type: Component
persistent identity: component1672958
display id: component1672958
version: 1
top level: ToxR-MalE
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1672950
display id: annotation1672950
version: 1
top level: ToxR-MalE
location: https://synbiohub.org/public/igem/BBa_J07077/annotation1672950/range1672950/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J07077/annotation1672950/range1672950/1
type: Range
persistent identity: range1672950
display id: range1672950
version: 1
top level: ToxR-MalE
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1672958
display id: annotation1672958
version: 1
top level: ToxR-MalE
location: https://synbiohub.org/public/igem/BBa_J07077/annotation1672958/range1672958/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07077/annotation1672958/range1672958/1
type: Range
persistent identity: range1672958
display id: range1672958
version: 1
top level: ToxR-MalE
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_J07009
type: SequenceAnnotation
persistent identity: annotation1672975
display id: annotation1672975
version: 1
top level: ToxR-MalE
location: https://synbiohub.org/public/igem/BBa_J07077/annotation1672975/range1672975/1
component: BBa_J07009

https://synbiohub.org/public/igem/BBa_J07077/annotation1672975/range1672975/1
type: Range
persistent identity: range1672975
display id: range1672975
version: 1
top level: ToxR-MalE
direction: direction/forward
start: 81
end: 710
orientation: inline

BBa_J07006
type: SequenceAnnotation
persistent identity: annotation1672980
display id: annotation1672980
version: 1
top level: ToxR-MalE
location: https://synbiohub.org/public/igem/BBa_J07077/annotation1672980/range1672980/1
component: BBa_J07006

https://synbiohub.org/public/igem/BBa_J07077/annotation1672980/range1672980/1
type: Range
persistent identity: range1672980
display id: range1672980
version: 1
top level: ToxR-MalE
direction: direction/forward
start: 717
end: 1907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1672985
display id: annotation1672985
version: 1
top level: ToxR-MalE
location: https://synbiohub.org/public/igem/BBa_J07077/annotation1672985/range1672985/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07077/annotation1672985/range1672985/1
type: Range
persistent identity: range1672985
display id: range1672985
version: 1
top level: ToxR-MalE
direction: direction/forward
start: 1916
end: 1995
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1672995
display id: annotation1672995
version: 1
top level: ToxR-MalE
location: https://synbiohub.org/public/igem/BBa_J07077/annotation1672995/range1672995/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07077/annotation1672995/range1672995/1
type: Range
persistent identity: range1672995
display id: range1672995
version: 1
top level: ToxR-MalE
direction: direction/forward
start: 2004
end: 2044
orientation: inline

BBa_J07006
type: ComponentDefinition
persistent identity: BBa J07006
display id: BBa_J07006
version: 1
was generated by: iGEM to SBOL conversion
description: malE
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:19Z
mutable provenance: Genbank E.coli K-12 strain
owned by: james
owned by: myers
mutable description: malE - a monomer. This monomeric protein is used as a negative control when testing for dimerization.
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 379
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: igem_mit_2005
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: malE
sequence: https://synbiohub.org/public/igem/BBa_J07006_sequence/1

malE
type: SequenceAnnotation
persistent identity: annotation1569704
display id: annotation1569704
version: 1
top level: BBa_J07006
location: https://synbiohub.org/public/igem/BBa_J07006/annotation1569704/range1569704/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J07006/annotation1569704/range1569704/1
type: Range
persistent identity: range1569704
display id: range1569704
version: 1
top level: BBa_J07006
direction: direction/forward
start: 1
end: 1191
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_J07009
type: ComponentDefinition
persistent identity: BBa J07009
display id: BBa_J07009
version: 1
was generated by: iGEM to SBOL conversion
description: toxicity-gene activator from Vibrio cholerae
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done @ aa 211. explanation of ToxR domains @ http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf ELIMINATE XbaI SITE PLZZZZZ
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: mit_igem_2005
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cytoplasmic Region
sequence annotation: Coding Sequence for ToxR'
sequence annotation: Start Codon
sequence annotation: Transmembrane Region
sequence annotation: Periplasmic Region
sequence: https://synbiohub.org/public/igem/BBa_J07009_sequence/1

Cytoplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668506
display id: annotation1668506
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
type: Range
persistent identity: range1668506
display id: range1668506
version: 1
top level: BBa_J07009
direction: direction/forward
start: 1
end: 546
orientation: inline

Coding Sequence for ToxR'
type: SequenceAnnotation
persistent identity: annotation1668507
display id: annotation1668507
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
type: Range
persistent identity: range1668507
display id: range1668507
version: 1
top level: BBa_J07009
direction: direction/forward
start: 1
end: 630
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1668508
display id: annotation1668508
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
type: Range
persistent identity: range1668508
display id: range1668508
version: 1
top level: BBa_J07009
direction: direction/forward
start: 6
end: 8
orientation: inline

Transmembrane Region
type: SequenceAnnotation
persistent identity: annotation1668509
display id: annotation1668509
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
type: Range
persistent identity: range1668509
display id: range1668509
version: 1
top level: BBa_J07009
direction: direction/forward
start: 547
end: 594
orientation: inline

Periplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668510
display id: annotation1668510
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
type: Range
persistent identity: range1668510
display id: range1668510
version: 1
top level: BBa_J07009
direction: direction/forward
start: 595
end: 630
orientation: inline

https://synbiohub.org/public/igem/BBa_J07077_sequence/1
type: Sequence
persistent identity: BBa J07077 sequence
display id: BBa_J07077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgtactagatgaaaataaaaacaggtgcacgcatcctcgcattatccgcattaacgacgatgatgttttccgcctcggctctcgccaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgccgccagtccgaacaaagagctggcgaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatccacgtattgccgccaccatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactcgtatcaccaagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07006_sequence/1
type: Sequence
persistent identity: BBa J07006 sequence
display id: BBa_J07006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaataaaaacaggtgcacgcatcctcgcattatccgcattaacgacgatgatgttttccgcctcggctctcgccaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgccgccagtccgaacaaagagctggcgaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatccacgtattgccgccaccatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactcgtatcaccaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07009_sequence/1
type: Sequence
persistent identity: BBa J07009 sequence
display id: BBa_J07009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z