LOCUS       BBa_J07080              1617 bp    DNA     linear       22-Dec-2024
DEFINITION  promotor rbs Toxr-scFv(iii) terminator
ACCESSION   BBa_J07080
VERSION     BBa_J07080.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..626
                     /label=Cytoplasmic Region
     CDS             81..710
                     /label=Coding Sequence for ToxR'
     misc_feature    86..88
                     /label=Start Codon
     misc_feature    627..674
                     /label=Transmembrane Region
     misc_feature    675..710
                     /label=Periplasmic Region
     CDS             81..710
                     /label=BBa_J07009
     misc_feature    779..838
                     /label=CDR1 VL
     misc_feature    881..901
                     /label=CDR2 VL
     misc_feature    998..1024
                     /label=CDR3 VL
     misc_feature    1055..1129
                     /label=linker
     misc_feature    1220..1231
                     /label=CDR1 VH
     misc_feature    1277..1333
                     /label=CDR2 VH
     misc_feature    1429..1450
                     /label=CDR3 VH
     misc_feature    719..1054
                     /label=VL
     misc_feature    1130..1480
                     /label=VH
     misc_feature    1430..1432
                     /label=S-A
     misc_feature    896..898
                     /label=F-V
     misc_feature    959..961
                     /label=S-N
     misc_feature    1130..1132
                     /label=D-G
     misc_feature    1175..1177
                     /label=R-G
     misc_feature    1178..1180
                     /label=P-A
     misc_feature    1199..1201
                     /label=A-T
     misc_feature    1217..1219
                     /label=S-G
     misc_feature    1220..1222
                     /label=D-H
     misc_feature    1280..1282
                     /label=I-F
     misc_feature    1406..1408
                     /label=M-T
     misc_feature    1430..1432
                     /label=S-A
     misc_feature    1433..1435
                     /label=Y-S
     misc_feature    1445..1447
                     /label=D-E
     misc_feature    1451..1453
                     /label=W-L
     misc_feature    719..1480
                     /label=BBa_J07014
     stem_loop       1500..1543
                     /label=stem_loop
     misc_feature    1489..1568
                     /label=BBa_B0010
     terminator      1489..1568
                     /label=BBa_B0010
     stem_loop       1584..1603
                     /label=T7 TE
     misc_feature    1610..1610
                     /label=stop
     polyA_site      1604..1617
                     /label=polya
     misc_feature    1577..1617
                     /label=BBa_B0012
     terminator      1577..1617
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgttcggat taggacacaa ctcaaaagag atatcgatga
      121 gtcatattgg tactaaattc attcttgctg aaaaatttac cttcgatccc ctaagcaata
      181 ctctgattga caaagaagat agtgaagaga tcattcgatt aggcagcaac gaaagccgaa
      241 tcctttggct gctggcccaa cgtccaaacg aggtaatttc tcgcaatgat ttgcatgact
      301 ttgtttggcg agagcaaggt tttgaagtcg atgattccag cttaacccaa gccatttcga
      361 ctctgcgcaa aatgctcaaa gattcgacaa agtccccaca atacgtcaaa acggttccga
      421 agcgcggtta ccaattgatc gcccgagtgg aaacggttga agaagagatg gctcgcgaaa
      481 acgaagctgc tcatgacatc tctcagccag aatctgtcaa tgaatacgca gaatcaagca
      541 gtgtgccttc atcagccact gtagtgaaca caccgcagcc agccaatgtc gtggcgaata
      601 aatcggctcc aaacttgggg aatcgactgt ttattctgat agcggtctta cttcccctcg
      661 cagtattact gctcactaac ccaagccaat ccagctttaa acccctaacg tactagagga
      721 cgtcgttatg actcaaacac cactatcact tcctgttagt ctaggtgatc aagcctccat
      781 ctcttgcaga tctagtcaga gccttgtaca cagtaatgga aacacctatt tacgttggta
      841 cctgcaaaag ccaggccagt ctccaaaggt cctgatctac aaagtttcca accgagtttc
      901 tggggtccca gacaggttca gtggcagtgg atcagggaca gatttcacac tcaagatcaa
      961 cagagtggag gctgaggatc tgggagttta tttctgctct caaagtacac atgttccgtg
     1021 gacgttcggt ggaggcacca agcttgaaat taagtcctct gctgatgatg ctaagaagga
     1081 tgctgctaag aaggatgatg ctaagaaaga tgatgctaag aaagatggtg gcgtcaaact
     1141 ggatgagact ggaggaggct tggtgcaacc tgggggggcc atgaaactct cctgtgttac
     1201 ctctggattc acttttggtc actactggat gaactgggtc cgccagtctc cagagaaagg
     1261 actggagtgg gtagcacaat ttagaaacaa accttataat tatgaaacat attattcaga
     1321 ttctgtgaaa ggcagattca ccatctcaag agatgattcc aaaagtagtg tctacctgca
     1381 aatgaacaac ttaagagttg aagacaccgg tatctattac tgtacgggtg cgtcttatgg
     1441 tatggaatac ttaggtcaag gaacctcagt caccgtctcc tactagagcc aggcatcaaa
     1501 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1561 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//