BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J07080 1 ToxR-scFv promotor rbs Toxr-scFv(iii) terminator 2005-08-26T11:00:00Z 2015-08-31T04:08:20Z promotor rbs Toxr-scFv(iii) terminator false false _21_ 0 363 21 Not in stock false false Will Bosworth component1673341 1 BBa_J07009 component1673430 1 BBa_B0012 component1673420 1 BBa_B0010 component1673316 1 BBa_R0040 component1673324 1 BBa_B0034 component1673415 1 BBa_J07014 annotation1673341 1 BBa_J07009 range1673341 1 81 710 annotation1673430 1 BBa_B0012 range1673430 1 1577 1617 annotation1673324 1 BBa_B0034 range1673324 1 63 74 annotation1673420 1 BBa_B0010 range1673420 1 1489 1568 annotation1673415 1 BBa_J07014 range1673415 1 719 1480 annotation1673316 1 BBa_R0040 range1673316 1 1 54 BBa_J07009 1 BBa_J07009 toxicity-gene activator from Vibrio cholerae 2005-07-06T11:00:00Z 2015-08-31T04:08:19Z shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done @ aa 211. explanation of ToxR domains @ http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf ELIMINATE XbaI SITE PLZZZZZ false false _21_ 0 363 21 In stock true true mit_igem_2005 annotation1668506 1 Cytoplasmic Region range1668506 1 1 546 annotation1668508 1 Start Codon range1668508 1 6 8 annotation1668510 1 Periplasmic Region range1668510 1 595 630 annotation1668507 1 Coding Sequence for ToxR' range1668507 1 1 630 annotation1668509 1 Transmembrane Region range1668509 1 547 594 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J07014 1 BBa_J07014 scFv anti-fluorescein [4M5.3] 2005-07-07T11:00:00Z 2015-08-31T04:08:19Z see J07013. single chain Antibody fragment selected to bind to fluorescein molecules. This scFv is the strongest of the three specified sequences. false false _21_ 0 363 21 It's complicated false false mit_igem_2005 annotation1583295 1 M-T range1583295 1 688 690 annotation1583296 1 S-A range1583296 1 712 714 annotation1583279 1 D-G range1583279 1 412 414 annotation1582328 1 CDR1 VH range1582328 1 502 513 annotation1583287 1 P-A range1583287 1 460 462 annotation1582322 1 CDR1 VL range1582322 1 61 120 annotation1582325 1 linker range1582325 1 337 411 annotation1582323 1 CDR2 VL range1582323 1 163 183 annotation1583278 1 S-N range1583278 1 241 243 annotation1583280 1 R-G range1583280 1 457 459 annotation1582336 1 VH range1582336 1 412 762 annotation1583294 1 I-F range1583294 1 562 564 annotation1583292 1 S-G range1583292 1 499 501 annotation1583288 1 A-T range1583288 1 481 483 annotation1582324 1 CDR3 VL range1582324 1 280 306 annotation1583299 1 W-L range1583299 1 733 735 annotation1582330 1 CDR2 VH range1582330 1 559 615 annotation1582335 1 VL range1582335 1 1 336 annotation1583293 1 D-H range1583293 1 502 504 annotation1583297 1 Y-S range1583297 1 715 717 annotation1582343 1 S-A range1582343 1 712 714 annotation1582331 1 CDR3 VH range1582331 1 711 732 annotation1583271 1 F-V range1583271 1 178 180 annotation1583298 1 D-E range1583298 1 727 729 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 BBa_J07009_sequence 1 atgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacg BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J07080_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgtactagaggacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctggattcacttttggtcactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggtatggaatacttaggtcaaggaacctcagtcaccgtctcctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_J07014_sequence 1 gacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctggattcacttttggtcactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggtatggaatacttaggtcaaggaacctcagtcaccgtctcc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z