ToxR-scFv
type: ComponentDefinition
persistent identity: BBa J07080
display id: BBa_J07080
version: 1
was generated by: iGEM to SBOL conversion
description: promotor rbs Toxr-scFv(iii) terminator
created: 2005-08-26T11:00:00Z
modified: 2015-08-31T04:08:20Z
owned by: james
owned by: myers
mutable description: promotor rbs Toxr-scFv(iii) terminator
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Will Bosworth
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0010
component: BBa_J07014
component: BBa_J07009
component: BBa_B0034
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_J07009
sequence annotation: BBa_J07014
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J07080_sequence/1

BBa_B0010
type: Component
persistent identity: component1673420
display id: component1673420
version: 1
top level: ToxR-scFv
definition: BBa_B0010
access: public

BBa_J07014
type: Component
persistent identity: component1673415
display id: component1673415
version: 1
top level: ToxR-scFv
definition: BBa_J07014
access: public

BBa_J07009
type: Component
persistent identity: component1673341
display id: component1673341
version: 1
top level: ToxR-scFv
definition: BBa_J07009
access: public

BBa_B0034
type: Component
persistent identity: component1673324
display id: component1673324
version: 1
top level: ToxR-scFv
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1673430
display id: component1673430
version: 1
top level: ToxR-scFv
definition: BBa_B0012
access: public

BBa_R0040
type: Component
persistent identity: component1673316
display id: component1673316
version: 1
top level: ToxR-scFv
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1673316
display id: annotation1673316
version: 1
top level: ToxR-scFv
location: https://synbiohub.org/public/igem/BBa_J07080/annotation1673316/range1673316/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J07080/annotation1673316/range1673316/1
type: Range
persistent identity: range1673316
display id: range1673316
version: 1
top level: ToxR-scFv
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1673324
display id: annotation1673324
version: 1
top level: ToxR-scFv
location: https://synbiohub.org/public/igem/BBa_J07080/annotation1673324/range1673324/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07080/annotation1673324/range1673324/1
type: Range
persistent identity: range1673324
display id: range1673324
version: 1
top level: ToxR-scFv
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_J07009
type: SequenceAnnotation
persistent identity: annotation1673341
display id: annotation1673341
version: 1
top level: ToxR-scFv
location: https://synbiohub.org/public/igem/BBa_J07080/annotation1673341/range1673341/1
component: BBa_J07009

https://synbiohub.org/public/igem/BBa_J07080/annotation1673341/range1673341/1
type: Range
persistent identity: range1673341
display id: range1673341
version: 1
top level: ToxR-scFv
direction: direction/forward
start: 81
end: 710
orientation: inline

BBa_J07014
type: SequenceAnnotation
persistent identity: annotation1673415
display id: annotation1673415
version: 1
top level: ToxR-scFv
location: https://synbiohub.org/public/igem/BBa_J07080/annotation1673415/range1673415/1
component: BBa_J07014

https://synbiohub.org/public/igem/BBa_J07080/annotation1673415/range1673415/1
type: Range
persistent identity: range1673415
display id: range1673415
version: 1
top level: ToxR-scFv
direction: direction/forward
start: 719
end: 1480
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1673420
display id: annotation1673420
version: 1
top level: ToxR-scFv
location: https://synbiohub.org/public/igem/BBa_J07080/annotation1673420/range1673420/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07080/annotation1673420/range1673420/1
type: Range
persistent identity: range1673420
display id: range1673420
version: 1
top level: ToxR-scFv
direction: direction/forward
start: 1489
end: 1568
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1673430
display id: annotation1673430
version: 1
top level: ToxR-scFv
location: https://synbiohub.org/public/igem/BBa_J07080/annotation1673430/range1673430/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07080/annotation1673430/range1673430/1
type: Range
persistent identity: range1673430
display id: range1673430
version: 1
top level: ToxR-scFv
direction: direction/forward
start: 1577
end: 1617
orientation: inline

BBa_J07014
type: ComponentDefinition
persistent identity: BBa J07014
display id: BBa_J07014
version: 1
was generated by: iGEM to SBOL conversion
description: scFv anti-fluorescein [4M5.3]
created: 2005-07-07T11:00:00Z
modified: 2015-08-31T04:08:19Z
mutable provenance: see J07013.
owned by: james
owned by: myers
mutable description: single chain Antibody fragment selected to bind to fluorescein molecules. This scFv is the strongest of the three specified sequences.
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: mit_igem_2005
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: VL
sequence annotation: CDR1 VL
sequence annotation: CDR2 VL
sequence annotation: F-V
sequence annotation: S-N
sequence annotation: CDR3 VL
sequence annotation: linker
sequence annotation: D-G
sequence annotation: VH
sequence annotation: R-G
sequence annotation: P-A
sequence annotation: A-T
sequence annotation: S-G
sequence annotation: CDR1 VH
sequence annotation: D-H
sequence annotation: CDR2 VH
sequence annotation: I-F
sequence annotation: M-T
sequence annotation: CDR3 VH
sequence annotation: S-A
sequence annotation: Y-S
sequence annotation: D-E
sequence annotation: W-L
sequence: https://synbiohub.org/public/igem/BBa_J07014_sequence/1

VL
type: SequenceAnnotation
persistent identity: annotation1582335
display id: annotation1582335
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582335/range1582335/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J07014/annotation1582335/range1582335/1
type: Range
persistent identity: range1582335
display id: range1582335
version: 1
top level: BBa_J07014
direction: direction/forward
start: 1
end: 336
orientation: inline

CDR1 VL
type: SequenceAnnotation
persistent identity: annotation1582322
display id: annotation1582322
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582322/range1582322/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07014/annotation1582322/range1582322/1
type: Range
persistent identity: range1582322
display id: range1582322
version: 1
top level: BBa_J07014
direction: direction/forward
start: 61
end: 120
orientation: inline

CDR2 VL
type: SequenceAnnotation
persistent identity: annotation1582323
display id: annotation1582323
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582323/range1582323/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07014/annotation1582323/range1582323/1
type: Range
persistent identity: range1582323
display id: range1582323
version: 1
top level: BBa_J07014
direction: direction/forward
start: 163
end: 183
orientation: inline

F-V
type: SequenceAnnotation
persistent identity: annotation1583271
display id: annotation1583271
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583271/range1583271/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1583271/range1583271/1
type: Range
persistent identity: range1583271
display id: range1583271
version: 1
top level: BBa_J07014
direction: direction/forward
start: 178
end: 180
orientation: inline

S-N
type: SequenceAnnotation
persistent identity: annotation1583278
display id: annotation1583278
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583278/range1583278/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1583278/range1583278/1
type: Range
persistent identity: range1583278
display id: range1583278
version: 1
top level: BBa_J07014
direction: direction/forward
start: 241
end: 243
orientation: inline

CDR3 VL
type: SequenceAnnotation
persistent identity: annotation1582324
display id: annotation1582324
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582324/range1582324/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07014/annotation1582324/range1582324/1
type: Range
persistent identity: range1582324
display id: range1582324
version: 1
top level: BBa_J07014
direction: direction/forward
start: 280
end: 306
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1582325
display id: annotation1582325
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582325/range1582325/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07014/annotation1582325/range1582325/1
type: Range
persistent identity: range1582325
display id: range1582325
version: 1
top level: BBa_J07014
direction: direction/forward
start: 337
end: 411
orientation: inline

D-G
type: SequenceAnnotation
persistent identity: annotation1583279
display id: annotation1583279
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583279/range1583279/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1583279/range1583279/1
type: Range
persistent identity: range1583279
display id: range1583279
version: 1
top level: BBa_J07014
direction: direction/forward
start: 412
end: 414
orientation: inline

VH
type: SequenceAnnotation
persistent identity: annotation1582336
display id: annotation1582336
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582336/range1582336/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07014/annotation1582336/range1582336/1
type: Range
persistent identity: range1582336
display id: range1582336
version: 1
top level: BBa_J07014
direction: direction/forward
start: 412
end: 762
orientation: inline

R-G
type: SequenceAnnotation
persistent identity: annotation1583280
display id: annotation1583280
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583280/range1583280/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1583280/range1583280/1
type: Range
persistent identity: range1583280
display id: range1583280
version: 1
top level: BBa_J07014
direction: direction/forward
start: 457
end: 459
orientation: inline

P-A
type: SequenceAnnotation
persistent identity: annotation1583287
display id: annotation1583287
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583287/range1583287/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1583287/range1583287/1
type: Range
persistent identity: range1583287
display id: range1583287
version: 1
top level: BBa_J07014
direction: direction/forward
start: 460
end: 462
orientation: inline

A-T
type: SequenceAnnotation
persistent identity: annotation1583288
display id: annotation1583288
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583288/range1583288/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J07014/annotation1583288/range1583288/1
type: Range
persistent identity: range1583288
display id: range1583288
version: 1
top level: BBa_J07014
direction: direction/forward
start: 481
end: 483
orientation: inline

S-G
type: SequenceAnnotation
persistent identity: annotation1583292
display id: annotation1583292
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583292/range1583292/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J07014/annotation1583292/range1583292/1
type: Range
persistent identity: range1583292
display id: range1583292
version: 1
top level: BBa_J07014
direction: direction/forward
start: 499
end: 501
orientation: inline

CDR1 VH
type: SequenceAnnotation
persistent identity: annotation1582328
display id: annotation1582328
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582328/range1582328/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07014/annotation1582328/range1582328/1
type: Range
persistent identity: range1582328
display id: range1582328
version: 1
top level: BBa_J07014
direction: direction/forward
start: 502
end: 513
orientation: inline

D-H
type: SequenceAnnotation
persistent identity: annotation1583293
display id: annotation1583293
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583293/range1583293/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J07014/annotation1583293/range1583293/1
type: Range
persistent identity: range1583293
display id: range1583293
version: 1
top level: BBa_J07014
direction: direction/forward
start: 502
end: 504
orientation: inline

CDR2 VH
type: SequenceAnnotation
persistent identity: annotation1582330
display id: annotation1582330
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582330/range1582330/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07014/annotation1582330/range1582330/1
type: Range
persistent identity: range1582330
display id: range1582330
version: 1
top level: BBa_J07014
direction: direction/forward
start: 559
end: 615
orientation: inline

I-F
type: SequenceAnnotation
persistent identity: annotation1583294
display id: annotation1583294
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583294/range1583294/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J07014/annotation1583294/range1583294/1
type: Range
persistent identity: range1583294
display id: range1583294
version: 1
top level: BBa_J07014
direction: direction/forward
start: 562
end: 564
orientation: inline

M-T
type: SequenceAnnotation
persistent identity: annotation1583295
display id: annotation1583295
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583295/range1583295/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J07014/annotation1583295/range1583295/1
type: Range
persistent identity: range1583295
display id: range1583295
version: 1
top level: BBa_J07014
direction: direction/forward
start: 688
end: 690
orientation: inline

CDR3 VH
type: SequenceAnnotation
persistent identity: annotation1582331
display id: annotation1582331
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582331/range1582331/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07014/annotation1582331/range1582331/1
type: Range
persistent identity: range1582331
display id: range1582331
version: 1
top level: BBa_J07014
direction: direction/forward
start: 711
end: 732
orientation: inline

S-A
type: SequenceAnnotation
persistent identity: annotation1583296
display id: annotation1583296
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583296/range1583296/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J07014/annotation1583296/range1583296/1
type: Range
persistent identity: range1583296
display id: range1583296
version: 1
top level: BBa_J07014
direction: direction/forward
start: 712
end: 714
orientation: inline

S-A
type: SequenceAnnotation
persistent identity: annotation1582343
display id: annotation1582343
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582343/range1582343/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1582343/range1582343/1
type: Range
persistent identity: range1582343
display id: range1582343
version: 1
top level: BBa_J07014
direction: direction/forward
start: 712
end: 714
orientation: inline

Y-S
type: SequenceAnnotation
persistent identity: annotation1583297
display id: annotation1583297
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583297/range1583297/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1583297/range1583297/1
type: Range
persistent identity: range1583297
display id: range1583297
version: 1
top level: BBa_J07014
direction: direction/forward
start: 715
end: 717
orientation: inline

D-E
type: SequenceAnnotation
persistent identity: annotation1583298
display id: annotation1583298
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583298/range1583298/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1583298/range1583298/1
type: Range
persistent identity: range1583298
display id: range1583298
version: 1
top level: BBa_J07014
direction: direction/forward
start: 727
end: 729
orientation: inline

W-L
type: SequenceAnnotation
persistent identity: annotation1583299
display id: annotation1583299
version: 1
top level: BBa_J07014
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583299/range1583299/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J07014/annotation1583299/range1583299/1
type: Range
persistent identity: range1583299
display id: range1583299
version: 1
top level: BBa_J07014
direction: direction/forward
start: 733
end: 735
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_J07009
type: ComponentDefinition
persistent identity: BBa J07009
display id: BBa_J07009
version: 1
was generated by: iGEM to SBOL conversion
description: toxicity-gene activator from Vibrio cholerae
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done @ aa 211. explanation of ToxR domains @ http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf ELIMINATE XbaI SITE PLZZZZZ
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: mit_igem_2005
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cytoplasmic Region
sequence annotation: Coding Sequence for ToxR'
sequence annotation: Start Codon
sequence annotation: Transmembrane Region
sequence annotation: Periplasmic Region
sequence: https://synbiohub.org/public/igem/BBa_J07009_sequence/1

Cytoplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668506
display id: annotation1668506
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
type: Range
persistent identity: range1668506
display id: range1668506
version: 1
top level: BBa_J07009
direction: direction/forward
start: 1
end: 546
orientation: inline

Coding Sequence for ToxR'
type: SequenceAnnotation
persistent identity: annotation1668507
display id: annotation1668507
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
type: Range
persistent identity: range1668507
display id: range1668507
version: 1
top level: BBa_J07009
direction: direction/forward
start: 1
end: 630
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1668508
display id: annotation1668508
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
type: Range
persistent identity: range1668508
display id: range1668508
version: 1
top level: BBa_J07009
direction: direction/forward
start: 6
end: 8
orientation: inline

Transmembrane Region
type: SequenceAnnotation
persistent identity: annotation1668509
display id: annotation1668509
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
type: Range
persistent identity: range1668509
display id: range1668509
version: 1
top level: BBa_J07009
direction: direction/forward
start: 547
end: 594
orientation: inline

Periplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668510
display id: annotation1668510
version: 1
top level: BBa_J07009
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
type: Range
persistent identity: range1668510
display id: range1668510
version: 1
top level: BBa_J07009
direction: direction/forward
start: 595
end: 630
orientation: inline

https://synbiohub.org/public/igem/BBa_J07080_sequence/1
type: Sequence
persistent identity: BBa J07080 sequence
display id: BBa_J07080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgtactagaggacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctggattcacttttggtcactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggtatggaatacttaggtcaaggaacctcagtcaccgtctcctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07014_sequence/1
type: Sequence
persistent identity: BBa J07014 sequence
display id: BBa_J07014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctggattcacttttggtcactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggtatggaatacttaggtcaaggaacctcagtcaccgtctcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07009_sequence/1
type: Sequence
persistent identity: BBa J07009 sequence
display id: BBa_J07009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z