LOCUS BBa_J07081 4904 bp DNA linear 22-Dec-2024 DEFINITION tetR promoter+rbs+scfv-FecA 527+rbs+FecI+rbs+FecR'+terminator ACCESSION BBa_J07081 VERSION BBa_J07081.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 promoter 1..74 /label=BBa_J13002 CDS 81..3227 /label=BBa_J07052 RBS 3236..3250 /label=RBS-1\Strong RBS 3243..3247 /label=RBS misc_feature 3236..3250 /label=BBa_B0030 RBS 3236..3250 /label=BBa_B0030 CDS 3257..3784 /label=FecI CDS 3257..3784 /label=BBa_J07022 misc_feature 3236..3784 /label=BBa_J07068 RBS 3793..3807 /label=RBS-1\Strong RBS 3800..3804 /label=RBS misc_feature 3793..3807 /label=BBa_B0030 RBS 3793..3807 /label=BBa_B0030 CDS 3814..4767 /label=FecR CDS 3814..4767 /label=BBa_J07023 stem_loop 4787..4830 /label=stem_loop misc_feature 4776..4855 /label=BBa_B0010 terminator 4776..4855 /label=BBa_B0010 stem_loop 4871..4890 /label=T7 TE misc_feature 4897..4897 /label=stop polyA_site 4891..4904 /label=polya misc_feature 4864..4904 /label=BBa_B0012 terminator 4864..4904 /label=BBa_B0012 misc_feature 3793..4904 /label=BBa_J07072 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg 121 ttaacaccat tcgcctgagc ctgctgccgc tggccggtct ctcgttttcc gcttttgctg 181 cacaggttaa tatcgcaccg ggatcgctcg ataaagcgct caatcagtat gccgcacaca 241 gcggatttac cctctcggtt gacgccagcc tgacgcgcgg caagcagagc aacggcctgc 301 acggcgatta cgacgtcgag agcggcctgc aacaactgct ggacggcagc ggactgcaag 361 taaaaccgct gggaaataac agctggacgc tggagcccgc gcccgcacca aaagaagatg 421 ccctgaccgt ggtcggcgac tggctgggtg atgcgcgtga aaacgacgta tttgaacatg 481 ctggcgcgcg tgacgtgatc cgccgtgagg atttcgccaa aaccggcgca accaccatgc 541 gtgaggtact taaccgcatc cctggcgtca gcgcgccgga aaacaacggc accggcagcc 601 acgacctggc gatgaacttt ggcatccggg gcctgaaccc gcgcctcgcc agccgctcga 661 ccgtcctgat ggacggcatc cccgtcccct tcgcccctta cggtcagccg cagctttcac 721 tggctcccgt ttcgctcggc aacatggatg ccattgacgt ggtacgcggt ggtggtgcgg 781 tgcgttacgg accgcagagc gtgggcggcg tggtgaactt tgttacccgt gccattccgc 841 aggactttgg tatcgaggcg ggcgtggaag gtcagctcag cccaacctct tcacaaaaca 901 acccgaaaga gacgcacaac ctgatggtgg gcggcacagc ggacaacggt tttggcaccg 961 cgctgctcta ctccggcacg cgcggcagtg actggcgcga gcacagcgcc acccgcatcg 1021 acgacctgat gctgaaaagc aaatatgcgc cggatgaggt gcacaccttc aacagcctgc 1081 tgcaatatta cgacggtgaa gccgacatgc ccggtggcct gtctcgcgcg gattacgacg 1141 ccgatcgctg gcaatccacc cgcccgtatg accgcttctg gggtcgtcgc aagctggcga 1201 gcctgggcta ccagttccag ccagacagcc agcataaatt caacattcag gggttctaca 1261 cccaaaccct gcgcagcggc tacctggagc aaggcaaacg catcaccctc tcgccgcgta 1321 actactgggt gcgcggtatt gagccacgct acagccagat ctttatgatc ggcccttccg 1381 cgcacgaagt gggcgtgggc tatcgctatt tgaatgaatc aacgcatgaa atgcgttact 1441 acaccgccac cagcagcggg cagttgccgt ccggctcaag cccttacgac cgcgatacgc 1501 gttccggcac cgaggcgcac gcctggtatc tggatgacaa aatcgacatc ggcaactgga 1561 ccatcacgcc gggtatgcgt ttcgaacata tcgagtcata ccagaacaac gccatcacag 1621 gcacgcacga agaagtgagc tataacgcac cgcttccggc ggacgtcgtt atgactcaaa 1681 caccactatc acttcctgtt agtctaggtg atcaagcctc catctcttgc agatctagtc 1741 agagccttgt acacagtaat ggaaacacct atttacgttg gtacctgcaa aagccaggcc 1801 agtctccaaa ggtcctgatc tacaaagttt ccaaccgagt ttctggggtc ccagacaggt 1861 tcagtggcag tggatcaggg acagatttca cactcaagat caacagagtg gaggctgagg 1921 atctgggagt ttatttctgc tctcaaagta cacatgttcc gtggacgttc ggtggaggca 1981 ccaagcttga aattaagtcc tctgctgatg atgctaagaa ggatgctgct aagaaggatg 2041 atgctaagaa agatgatgct aagaaagatg gtggcgtcaa actggatgag actggaggag 2101 gcttggtgca acctgggggg gccatgaaac tctcctgtgt tacctctgga ttcacttttg 2161 gtcactactg gatgaactgg gtccgccagt ctccagagaa aggactggag tgggtagcac 2221 aatttagaaa caaaccttat aattatgaaa catattattc agattctgtg aaaggcagat 2281 tcaccatctc aagagatgat tccaaaagta gtgtctacct gcaaatgaac aacttaagag 2341 ttgaagacac cggtatctat tactgtacgg gtgcgtctta tggtatggaa tacttaggtc 2401 aaggaacctc agtcaccgtc tccccgaaca gcgcgagcca tagcggcagc gcgccgaaca 2461 ccagcagcgc gccgggcagc ttgaacgtgc tctatcacct gactgacagc tggaatcttt 2521 atgcaaacac tgaaggctcg ttcggcaccg tacagtacag ccagattggc aaggctgtgc 2581 aaagcggcaa tgttgaaccg gaaaaagcgc gaacctggga actcggtacc cgctacgacg 2641 acggcgcgct gacggcggaa atggggctgt tcctgattaa ctttaacaat cagtacgact 2701 ccaaccagac caacgacacc gtcactgcac gtggcaaaac gcgccatacc gggctggaaa 2761 cgcaggcacg ttacgatctg ggtacgctaa cgccaacgct tgataacgtt tccatctacg 2821 ccagctatgc gtatgtgaac gcggaaatcc gcgagaaagg cgacacctac ggcaatctgg 2881 taccattctc cccgaaacat aaaggcacgc tgggcgtgga ctacaagcca ggaaactgga 2941 cgttcaatct gaacagcgat ttccagtcca gccagtttgc ggataacgcc aatacggtga 3001 aagagagcgc cgacggcagt accggccgca ttcccggctt catgctctgg ggcgcacgcg 3061 tggcgtatga ctttggcccg cagatggcag atctgaacct ggcattcggt gtgaaaaaca 3121 tcttcgacca ggactacttc atccgctctt atgacgacaa caacaaaggc atctatgcag 3181 gccagccgcg cacgctgtat atgcaggggt cgttgaagtt ctaataatac tagagattaa 3241 agaggagaaa tactagatgt ctgaccgcgc cactaccaca gcttccttaa cgttcgagtc 3301 gctttatggc acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc 3361 ttttgatgca gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct 3421 ctcgacgatc cgcgatcctc gctccttcct ctgcactatc gccaaacgcg tgatggtgga 3481 cctgtttcgc cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga 3541 ggggggagcg ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga 3601 cagcatgctg gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga 3661 tggtctgaca tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa 3721 atacgtggcg aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgata 3781 ataatactag agattaaaga ggagaaatac tagatgaatc ctttgttaac cgattcccgc 3841 cgtcaggcgc tgcgttcagc ttcccactgg tatgccgtgc taagcggcga gcgcgtcagc 3901 ccacaacagg aagcgcgctg gcaacagtgg tatgaacagg atcaggataa ccagtgggcc 3961 tggcagcagg ttgaaaacct gcgcaaccag cttggcggtg tgcctggcga cgttgccagc 4021 cgggcgttgc acgatacccg cctcacccgc cgtcacgtga tgaaaggatt actgctgttg 4081 ctcggcgctg gcggaggctg gcagctctgg cagtcggaaa ccggcgaagg tctgcgggca 4141 gattaccgca ccgccaaagg cacagtcagc cgtcagcaac tggaagatgg ctccctgctc 4201 acgctgaata cccaaagcgc ggcggatgtg cgttttgatg cgcatcagcg caccgtccgg 4261 ctctggtacg gtgaaatcgc cattactacc gcgaaagatg ccctgcaacg ccccttccgt 4321 gtcctgaccc gtcagggcca gctcactgct ttagggacag aatttaccgt ccgccagcag 4381 gataatttca cgcagcttga cgtgcagcag cacgctgtgg aagtgcttct cgccagtgcc 4441 cccgcgcaaa aacgcatcgt gaacgctggt gaaagcctac agttcagcgc ctctgagttt 4501 ggcgcagtga aaccgctgga tgacgagagt acaagctgga cgaaggacat cctgagcttc 4561 agcgataaac cgctgggtga ggtgatagcc acgctaaccc gttaccgcaa cggcgtgctg 4621 cgctgcgatc ccgccgttgc cgggctgcgc ctgagcggga cgttcccgct gaaaaatacc 4681 gatgcgatcc tgaacgttat cgcgcaaacg cttcccgtta aaattcagtc tattacgcgg 4741 tactggataa acatttcacc actgtaatac tagagccagg catcaaataa aacgaaaggc 4801 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 4861 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //