LOCUS BBa_J07082 4904 bp DNA linear 17-May-2024 DEFINITION tetR promoter+rbs+scfv-FecA 155+rbs+FecI+rbs+FecR'+terminator ACCESSION BBa_J07082 VERSION BBa_J07082.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 promoter 1..74 /label=BBa_J13002 misc_feature 81..100 /label=EcoRI & XbaI CDS 101..565 /label=FecA aa 1-155 CDS 566..1327 /label=scFv misc_feature 1328..1384 /label=external linker CDS 1385..3244 /label=FecA aa 156-755 misc_feature 3245..3265 /label=SpeI & PstI CDS 81..3227 /label=BBa_J07048 RBS 3236..3250 /label=RBS-1\Strong RBS 3243..3247 /label=RBS misc_feature 3236..3250 /label=BBa_B0030 RBS 3236..3250 /label=BBa_B0030 CDS 3257..3784 /label=FecI CDS 3257..3784 /label=BBa_J07022 misc_feature 3236..3784 /label=BBa_J07068 RBS 3793..3807 /label=RBS-1\Strong RBS 3800..3804 /label=RBS misc_feature 3793..3807 /label=BBa_B0030 RBS 3793..3807 /label=BBa_B0030 CDS 3814..4767 /label=FecR CDS 3814..4767 /label=BBa_J07023 stem_loop 4787..4830 /label=stem_loop misc_feature 4776..4855 /label=BBa_B0010 terminator 4776..4855 /label=BBa_B0010 stem_loop 4871..4890 /label=T7 TE misc_feature 4897..4897 /label=stop polyA_site 4891..4904 /label=polya misc_feature 4864..4904 /label=BBa_B0012 terminator 4864..4904 /label=BBa_B0012 misc_feature 3793..4904 /label=BBa_J07072 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg 121 ttaacaccat tcgcctgagc ctgctgccgc tggccggtct ctcgttttcc gcttttgctg 181 cacaggttaa tatcgcaccg ggatcgctcg ataaagcgct caatcagtat gccgcacaca 241 gcggatttac cctctcggtt gacgccagcc tgacgcgcgg caagcagagc aacggcctgc 301 acggcgatta cgacgtcgag agcggcctgc aacaactgct ggacggcagc ggactgcaag 361 taaaaccgct gggaaataac agctggacgc tggagcccgc gcccgcacca aaagaagatg 421 ccctgaccgt ggtcggcgac tggctgggtg atgcgcgtga aaacgacgta tttgaacatg 481 ctggcgcgcg tgacgtgatc cgccgtgagg atttcgccaa aaccggcgca accaccatgc 541 gtgaggacgt cgttatgact caaacaccac tatcacttcc tgttagtcta ggtgatcaag 601 cctccatctc ttgcagatct agtcagagcc ttgtacacag taatggaaac acctatttac 661 gttggtacct gcaaaagcca ggccagtctc caaaggtcct gatctacaaa gtttccaacc 721 gagtttctgg ggtcccagac aggttcagtg gcagtggatc agggacagat ttcacactca 781 agatcaacag agtggaggct gaggatctgg gagtttattt ctgctctcaa agtacacatg 841 ttccgtggac gttcggtgga ggcaccaagc ttgaaattaa gtcctctgct gatgatgcta 901 agaaggatgc tgctaagaag gatgatgcta agaaagatga tgctaagaaa gatggtggcg 961 tcaaactgga tgagactgga ggaggcttgg tgcaacctgg gggggccatg aaactctcct 1021 gtgttacctc tggattcact tttggtcact actggatgaa ctgggtccgc cagtctccag 1081 agaaaggact ggagtgggta gcacaattta gaaacaaacc ttataattat gaaacatatt 1141 attcagattc tgtgaaaggc agattcacca tctcaagaga tgattccaaa agtagtgtct 1201 acctgcaaat gaacaactta agagttgaag acaccggtat ctattactgt acgggtgcgt 1261 cttatggtat ggaatactta ggtcaaggaa cctcagtcac cgtctccccg aacagcgcga 1321 gccatagcgg cagcgcgccg aacaccagca gcgcgccggg cagcgtactt aaccgcatcc 1381 ctggcgtcag cgcgccggaa aacaacggca ccggcagcca cgacctggcg atgaactttg 1441 gcatccgggg cctgaacccg cgcctcgcca gccgctcgac cgtcctgatg gacggcatcc 1501 ccgtcccctt cgccccttac ggtcagccgc agctttcact ggctcccgtt tcgctcggca 1561 acatggatgc cattgacgtg gtacgcggtg gtggtgcggt gcgttacgga ccgcagagcg 1621 tgggcggcgt ggtgaacttt gttacccgtg ccattccgca ggactttggt atcgaggcgg 1681 gcgtggaagg tcagctcagc ccaacctctt cacaaaacaa cccgaaagag acgcacaacc 1741 tgatggtggg cggcacagcg gacaacggtt ttggcaccgc gctgctctac tccggcacgc 1801 gcggcagtga ctggcgcgag cacagcgcca cccgcatcga cgacctgatg ctgaaaagca 1861 aatatgcgcc ggatgaggtg cacaccttca acagcctgct gcaatattac gacggtgaag 1921 ccgacatgcc cggtggcctg tctcgcgcgg attacgacgc cgatcgctgg caatccaccc 1981 gcccgtatga ccgcttctgg ggtcgtcgca agctggcgag cctgggctac cagttccagc 2041 cagacagcca gcataaattc aacattcagg ggttctacac ccaaaccctg cgcagcggct 2101 acctggagca aggcaaacgc atcaccctct cgccgcgtaa ctactgggtg cgcggtattg 2161 agccacgcta cagccagatc tttatgatcg gcccttccgc gcacgaagtg ggcgtgggct 2221 atcgctattt gaatgaatca acgcatgaaa tgcgttacta caccgccacc agcagcgggc 2281 agttgccgtc cggctcaagc ccttacgacc gcgatacgcg ttccggcacc gaggcgcacg 2341 cctggtatct ggatgacaaa atcgacatcg gcaactggac catcacgccg ggtatgcgtt 2401 tcgaacatat cgagtcatac cagaacaacg ccatcacagg cacgcacgaa gaagtgagct 2461 ataacgcacc gcttccggcg ttgaacgtgc tctatcacct gactgacagc tggaatcttt 2521 atgcaaacac tgaaggctcg ttcggcaccg tacagtacag ccagattggc aaggctgtgc 2581 aaagcggcaa tgttgaaccg gaaaaagcgc gaacctggga actcggtacc cgctacgacg 2641 acggcgcgct gacggcggaa atggggctgt tcctgattaa ctttaacaat cagtacgact 2701 ccaaccagac caacgacacc gtcactgcac gtggcaaaac gcgccatacc gggctggaaa 2761 cgcaggcacg ttacgatctg ggtacgctaa cgccaacgct tgataacgtt tccatctacg 2821 ccagctatgc gtatgtgaac gcggaaatcc gcgagaaagg cgacacctac ggcaatctgg 2881 taccattctc cccgaaacat aaaggcacgc tgggcgtgga ctacaagcca ggaaactgga 2941 cgttcaatct gaacagcgat ttccagtcca gccagtttgc ggataacgcc aatacggtga 3001 aagagagcgc cgacggcagt accggccgca ttcccggctt catgctctgg ggcgcacgcg 3061 tggcgtatga ctttggcccg cagatggcag atctgaacct ggcattcggt gtgaaaaaca 3121 tcttcgacca ggactacttc atccgctctt atgacgacaa caacaaaggc atctatgcag 3181 gccagccgcg cacgctgtat atgcaggggt cgttgaagtt ctaataatac tagagattaa 3241 agaggagaaa tactagatgt ctgaccgcgc cactaccaca gcttccttaa cgttcgagtc 3301 gctttatggc acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc 3361 ttttgatgca gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct 3421 ctcgacgatc cgcgatcctc gctccttcct ctgcactatc gccaaacgcg tgatggtgga 3481 cctgtttcgc cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga 3541 ggggggagcg ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga 3601 cagcatgctg gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga 3661 tggtctgaca tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa 3721 atacgtggcg aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgata 3781 ataatactag agattaaaga ggagaaatac tagatgaatc ctttgttaac cgattcccgc 3841 cgtcaggcgc tgcgttcagc ttcccactgg tatgccgtgc taagcggcga gcgcgtcagc 3901 ccacaacagg aagcgcgctg gcaacagtgg tatgaacagg atcaggataa ccagtgggcc 3961 tggcagcagg ttgaaaacct gcgcaaccag cttggcggtg tgcctggcga cgttgccagc 4021 cgggcgttgc acgatacccg cctcacccgc cgtcacgtga tgaaaggatt actgctgttg 4081 ctcggcgctg gcggaggctg gcagctctgg cagtcggaaa ccggcgaagg tctgcgggca 4141 gattaccgca ccgccaaagg cacagtcagc cgtcagcaac tggaagatgg ctccctgctc 4201 acgctgaata cccaaagcgc ggcggatgtg cgttttgatg cgcatcagcg caccgtccgg 4261 ctctggtacg gtgaaatcgc cattactacc gcgaaagatg ccctgcaacg ccccttccgt 4321 gtcctgaccc gtcagggcca gctcactgct ttagggacag aatttaccgt ccgccagcag 4381 gataatttca cgcagcttga cgtgcagcag cacgctgtgg aagtgcttct cgccagtgcc 4441 cccgcgcaaa aacgcatcgt gaacgctggt gaaagcctac agttcagcgc ctctgagttt 4501 ggcgcagtga aaccgctgga tgacgagagt acaagctgga cgaaggacat cctgagcttc 4561 agcgataaac cgctgggtga ggtgatagcc acgctaaccc gttaccgcaa cggcgtgctg 4621 cgctgcgatc ccgccgttgc cgggctgcgc ctgagcggga cgttcccgct gaaaaatacc 4681 gatgcgatcc tgaacgttat cgcgcaaacg cttcccgtta aaattcagtc tattacgcgg 4741 tactggataa acatttcacc actgtaatac tagagccagg catcaaataa aacgaaaggc 4801 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 4861 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //