##gff-version 3
##sequence-region BBa_J07084 1 4904
BBa_J07084	.	sequence_feature	3793	4904	.	+	0	ID=BBa_J07072;Name=BBa_J07072
BBa_J07084	.	terminator	4864	4904	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J07072
BBa_J07084	.	stop_codon	4897	4897	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07084	.	polyA_site	4891	4904	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07084	.	stem_loop	4871	4890	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07084	.	engineered_region	4864	4904	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07084	.	CDS	3814	4767	.	+	0	ID=BBa_J07023;Name=BBa_J07023;Parent=BBa_J07072
BBa_J07084	.	CDS	3814	4767	.	+	0	ID=annotation1577975;Name=FecR;Parent=BBa_J07023
BBa_J07084	.	terminator	4776	4855	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J07072
BBa_J07084	.	engineered_region	4776	4855	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07084	.	stem_loop	4787	4830	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07084	.	ribosome_entry_site	3793	3807	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J07072
BBa_J07084	.	ribosome_entry_site	3800	3804	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07084	.	engineered_region	3793	3807	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07084	.	ribosome_entry_site	3793	3807	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J07084	.	sequence_feature	3236	3784	.	+	0	ID=BBa_J07068;Name=BBa_J07068
BBa_J07084	.	ribosome_entry_site	3236	3250	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J07068
BBa_J07084	.	ribosome_entry_site	3243	3247	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07084	.	engineered_region	3236	3250	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07084	.	ribosome_entry_site	3236	3250	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J07084	.	CDS	3257	3784	.	+	0	ID=BBa_J07022;Name=BBa_J07022;Parent=BBa_J07068
BBa_J07084	.	CDS	3257	3784	.	+	0	ID=annotation1577017;Name=FecI;Parent=BBa_J07022
BBa_J07084	.	promoter	1	74	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_J07084	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_J07084	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07084	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_J07084	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07084	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J07084	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07084	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07084	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07084	.	CDS	81	3227	.	+	0	ID=BBa_J07049;Name=BBa_J07049
BBa_J07084	.	CDS	1448	3244	.	+	0	ID=annotation1699266;Name=FecA aa 176-755;Parent=BBa_J07049
BBa_J07084	.	sequence_feature	1391	1447	.	+	0	ID=annotation1699265;Name=linker;Parent=BBa_J07049
BBa_J07084	.	engineered_region	81	100	.	+	0	ID=annotation1699262;Name=EcoRI & XbaI;Parent=BBa_J07049
BBa_J07084	.	CDS	629	1390	.	+	0	ID=annotation1699264;Name=scFv;Parent=BBa_J07049
BBa_J07084	.	CDS	101	628	.	+	0	ID=annotation1699263;Name=FecA aa 1-176;Parent=BBa_J07049
BBa_J07084	.	engineered_region	3245	3265	.	+	0	ID=annotation1699267;Name=SpeI & PstI;Parent=BBa_J07049
>BBa_J07084
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgacgccgttacgcgtttttcgtaaaacaacacctttggttaacaccattcgcctgagcctgctgccgctggccggt
ctctcgttttccgcttttgctgcacaggttaatatcgcaccgggatcgctcgataaagcgctcaatcagtatgccgcac
acagcggatttaccctctcggttgacgccagcctgacgcgcggcaagcagagcaacggcctgcacggcgattacgacgt
cgagagcggcctgcaacaactgctggacggcagcggactgcaagtaaaaccgctgggaaataacagctggacgctggag
cccgcgcccgcaccaaaagaagatgccctgaccgtggtcggcgactggctgggtgatgcgcgtgaaaacgacgtatttg
aacatgctggcgcgcgtgacgtgatccgccgtgaggatttcgccaaaaccggcgcaaccaccatgcgtgaggtacttaa
ccgcatccctggcgtcagcgcgccggaaaacaacggcaccggcagccacgacctggacgtcgttatgactcaaacacca
ctatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaa
acacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttc
tggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggat
ctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcct
ctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtggcgtcaa
actggatgagactggaggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctggattcactttt
ggtcactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttagaaacaaacctt
ataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtcta
cctgcaaatgaacaacttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggtatggaatactta
ggtcaaggaacctcagtcaccgtctccccgaacagcgcgagccatagcggcagcgcgccgaacaccagcagcgcgccgg
gcagcgcgatgaactttggcatccggggcctgaacccgcgcctcgccagccgctcgaccgtcctgatggacggcatccc
cgtccccttcgccccttacggtcagccgcagctttcactggctcccgtttcgctcggcaacatggatgccattgacgtg
gtacgcggtggtggtgcggtgcgttacggaccgcagagcgtgggcggcgtggtgaactttgttacccgtgccattccgc
aggactttggtatcgaggcgggcgtggaaggtcagctcagcccaacctcttcacaaaacaacccgaaagagacgcacaa
cctgatggtgggcggcacagcggacaacggttttggcaccgcgctgctctactccggcacgcgcggcagtgactggcgc
gagcacagcgccacccgcatcgacgacctgatgctgaaaagcaaatatgcgccggatgaggtgcacaccttcaacagcc
tgctgcaatattacgacggtgaagccgacatgcccggtggcctgtctcgcgcggattacgacgccgatcgctggcaatc
cacccgcccgtatgaccgcttctggggtcgtcgcaagctggcgagcctgggctaccagttccagccagacagccagcat
aaattcaacattcaggggttctacacccaaaccctgcgcagcggctacctggagcaaggcaaacgcatcaccctctcgc
cgcgtaactactgggtgcgcggtattgagccacgctacagccagatctttatgatcggcccttccgcgcacgaagtggg
cgtgggctatcgctatttgaatgaatcaacgcatgaaatgcgttactacaccgccaccagcagcgggcagttgccgtcc
ggctcaagcccttacgaccgcgatacgcgttccggcaccgaggcgcacgcctggtatctggatgacaaaatcgacatcg
gcaactggaccatcacgccgggtatgcgtttcgaacatatcgagtcataccagaacaacgccatcacaggcacgcacga
agaagtgagctataacgcaccgcttccggcgttgaacgtgctctatcacctgactgacagctggaatctttatgcaaac
actgaaggctcgttcggcaccgtacagtacagccagattggcaaggctgtgcaaagcggcaatgttgaaccggaaaaag
cgcgaacctgggaactcggtacccgctacgacgacggcgcgctgacggcggaaatggggctgttcctgattaactttaa
caatcagtacgactccaaccagaccaacgacaccgtcactgcacgtggcaaaacgcgccataccgggctggaaacgcag
gcacgttacgatctgggtacgctaacgccaacgcttgataacgtttccatctacgccagctatgcgtatgtgaacgcgg
aaatccgcgagaaaggcgacacctacggcaatctggtaccattctccccgaaacataaaggcacgctgggcgtggacta
caagccaggaaactggacgttcaatctgaacagcgatttccagtccagccagtttgcggataacgccaatacggtgaaa
gagagcgccgacggcagtaccggccgcattcccggcttcatgctctggggcgcacgcgtggcgtatgactttggcccgc
agatggcagatctgaacctggcattcggtgtgaaaaacatcttcgaccaggactacttcatccgctcttatgacgacaa
caacaaaggcatctatgcaggccagccgcgcacgctgtatatgcaggggtcgttgaagttctaataatactagagatta
aagaggagaaatactagatgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatca
cggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttg
cgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatgg
tggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttc
acctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgt
gaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccg
tgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataatactagag
attaaagaggagaaatactagatgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggt
atgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataa
ccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttg
cacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctct
ggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaaga
tggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctgg
tacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctca
ctgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagt
gcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggc
gcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgagg
tgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggac
gttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcgg
tactggataaacatttcaccactgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata