LOCUS BBa_J07085 4877 bp DNA linear 20-Nov-2024 DEFINITION tetR promoter+rbs+scfv-FecA 522-532+rbs+FecI+rbs+FecR ACCESSION BBa_J07085 VERSION BBa_J07085.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 promoter 1..74 /label=BBa_J13002 CDS 81..3200 /label=BBa_J07060 RBS 3209..3223 /label=RBS-1\Strong RBS 3216..3220 /label=RBS misc_feature 3209..3223 /label=BBa_B0030 RBS 3209..3223 /label=BBa_B0030 CDS 3230..3757 /label=FecI CDS 3230..3757 /label=BBa_J07022 misc_feature 3209..3757 /label=BBa_J07068 RBS 3766..3780 /label=RBS-1\Strong RBS 3773..3777 /label=RBS misc_feature 3766..3780 /label=BBa_B0030 RBS 3766..3780 /label=BBa_B0030 CDS 3787..4740 /label=FecR CDS 3787..4740 /label=BBa_J07023 stem_loop 4760..4803 /label=stem_loop misc_feature 4749..4828 /label=BBa_B0010 terminator 4749..4828 /label=BBa_B0010 stem_loop 4844..4863 /label=T7 TE misc_feature 4870..4870 /label=stop polyA_site 4864..4877 /label=polya misc_feature 4837..4877 /label=BBa_B0012 terminator 4837..4877 /label=BBa_B0012 misc_feature 3766..4877 /label=BBa_J07072 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg 121 ttaacaccat tcgcctgagc ctgctgccgc tggccggtct ctcgttttcc gcttttgctg 181 cacaggttaa tatcgcaccg ggatcgctcg ataaagcgct caatcagtat gccgcacaca 241 gcggatttac cctctcggtt gacgccagcc tgacgcgcgg caagcagagc aacggcctgc 301 acggcgatta cgacgtcgag agcggcctgc aacaactgct ggacggcagc ggactgcaag 361 taaaaccgct gggaaataac agctggacgc tggagcccgc gcccgcacca aaagaagatg 421 ccctgaccgt ggtcggcgac tggctgggtg atgcgcgtga aaacgacgta tttgaacatg 481 ctggcgcgcg tgacgtgatc cgccgtgagg atttcgccaa aaccggcgca accaccatgc 541 gtgaggtact taaccgcatc cctggcgtca gcgcgccgga aaacaacggc accggcagcc 601 acgacctggc gatgaacttt ggcatccggg gcctgaaccc gcgcctcgcc agccgctcga 661 ccgtcctgat ggacggcatc cccgtcccct tcgcccctta cggtcagccg cagctttcac 721 tggctcccgt ttcgctcggc aacatggatg ccattgacgt ggtacgcggt ggtggtgcgg 781 tgcgttacgg accgcagagc gtgggcggcg tggtgaactt tgttacccgt gccattccgc 841 aggactttgg tatcgaggcg ggcgtggaag gtcagctcag cccaacctct tcacaaaaca 901 acccgaaaga gacgcacaac ctgatggtgg gcggcacagc ggacaacggt tttggcaccg 961 cgctgctcta ctccggcacg cgcggcagtg actggcgcga gcacagcgcc acccgcatcg 1021 acgacctgat gctgaaaagc aaatatgcgc cggatgaggt gcacaccttc aacagcctgc 1081 tgcaatatta cgacggtgaa gccgacatgc ccggtggcct gtctcgcgcg gattacgacg 1141 ccgatcgctg gcaatccacc cgcccgtatg accgcttctg gggtcgtcgc aagctggcga 1201 gcctgggcta ccagttccag ccagacagcc agcataaatt caacattcag gggttctaca 1261 cccaaaccct gcgcagcggc tacctggagc aaggcaaacg catcaccctc tcgccgcgta 1321 actactgggt gcgcggtatt gagccacgct acagccagat ctttatgatc ggcccttccg 1381 cgcacgaagt gggcgtgggc tatcgctatt tgaatgaatc aacgcatgaa atgcgttact 1441 acaccgccac cagcagcggg cagttgccgt ccggctcaag cccttacgac cgcgatacgc 1501 gttccggcac cgaggcgcac gcctggtatc tggatgacaa aatcgacatc ggcaactgga 1561 ccatcacgcc gggtatgcgt ttcgaacata tcgagtcata ccagaacaac gccatcacag 1621 gcacgcacga agaagtgagc tataacgacg tcgttatgac tcaaacacca ctatcacttc 1681 ctgttagtct aggtgatcaa gcctccatct cttgcagatc tagtcagagc cttgtacaca 1741 gtaatggaaa cacctattta cgttggtacc tgcaaaagcc aggccagtct ccaaaggtcc 1801 tgatctacaa agtttccaac cgagtttctg gggtcccaga caggttcagt ggcagtggat 1861 cagggacaga tttcacactc aagatcaaca gagtggaggc tgaggatctg ggagtttatt 1921 tctgctctca aagtacacat gttccgtgga cgttcggtgg aggcaccaag cttgaaatta 1981 agtcctctgc tgatgatgct aagaaggatg ctgctaagaa ggatgatgct aagaaagatg 2041 atgctaagaa agatggtggc gtcaaactgg atgagactgg aggaggcttg gtgcaacctg 2101 ggggggccat gaaactctcc tgtgttacct ctggattcac ttttggtcac tactggatga 2161 actgggtccg ccagtctcca gagaaaggac tggagtgggt agcacaattt agaaacaaac 2221 cttataatta tgaaacatat tattcagatt ctgtgaaagg cagattcacc atctcaagag 2281 atgattccaa aagtagtgtc tacctgcaaa tgaacaactt aagagttgaa gacaccggta 2341 tctattactg tacgggtgcg tcttatggta tggaatactt aggtcaagga acctcagtca 2401 ccgtctcccc gaacagcgcg agccatagcg gcagcgcgcc gaacaccagc agcgcgccgg 2461 gcagctatca cctgactgac agctggaatc tttatgcaaa cactgaaggc tcgttcggca 2521 ccgtacagta cagccagatt ggcaaggctg tgcaaagcgg caatgttgaa ccggaaaaag 2581 cgcgaacctg ggaactcggt acccgctacg acgacggcgc gctgacggcg gaaatggggc 2641 tgttcctgat taactttaac aatcagtacg actccaacca gaccaacgac accgtcactg 2701 cacgtggcaa aacgcgccat accgggctgg aaacgcaggc acgttacgat ctgggtacgc 2761 taacgccaac gcttgataac gtttccatct acgccagcta tgcgtatgtg aacgcggaaa 2821 tccgcgagaa aggcgacacc tacggcaatc tggtaccatt ctccccgaaa cataaaggca 2881 cgctgggcgt ggactacaag ccaggaaact ggacgttcaa tctgaacagc gatttccagt 2941 ccagccagtt tgcggataac gccaatacgg tgaaagagag cgccgacggc agtaccggcc 3001 gcattcccgg cttcatgctc tggggcgcac gcgtggcgta tgactttggc ccgcagatgg 3061 cagatctgaa cctggcattc ggtgtgaaaa acatcttcga ccaggactac ttcatccgct 3121 cttatgacga caacaacaaa ggcatctatg caggccagcc gcgcacgctg tatatgcagg 3181 ggtcgttgaa gttctaataa tactagagat taaagaggag aaatactaga tgtctgaccg 3241 cgccactacc acagcttcct taacgttcga gtcgctttat ggcacacatc acggctggtt 3301 gaaaagctgg ctgacgcgca aactccagtc tgcttttgat gcagatgaca ttgcccagga 3361 cacttttttg cgggtaatgg tcagcgaaac gctctcgacg atccgcgatc ctcgctcctt 3421 cctctgcact atcgccaaac gcgtgatggt ggacctgttt cgccgaaacg cgctggaaaa 3481 agcgtatctg gagatgctgg cgcttatgcc ggagggggga gcgccttcac ctgaggaacg 3541 cgaaagccaa ctcgagaccc tacaactcct cgacagcatg ctggacgggc taaacggcaa 3601 aacacgtgaa gcgtttctgc tttcgcaact ggatggtctg acatacagcg agattgcgca 3661 caaactcggt gtttccatca gctccgtgaa aaaatacgtg gcgaaagccg tcgagcactg 3721 cctgctgttc cgtctggagt atgggttatg ataataatac tagagattaa agaggagaaa 3781 tactagatga atcctttgtt aaccgattcc cgccgtcagg cgctgcgttc agcttcccac 3841 tggtatgccg tgctaagcgg cgagcgcgtc agcccacaac aggaagcgcg ctggcaacag 3901 tggtatgaac aggatcagga taaccagtgg gcctggcagc aggttgaaaa cctgcgcaac 3961 cagcttggcg gtgtgcctgg cgacgttgcc agccgggcgt tgcacgatac ccgcctcacc 4021 cgccgtcacg tgatgaaagg attactgctg ttgctcggcg ctggcggagg ctggcagctc 4081 tggcagtcgg aaaccggcga aggtctgcgg gcagattacc gcaccgccaa aggcacagtc 4141 agccgtcagc aactggaaga tggctccctg ctcacgctga atacccaaag cgcggcggat 4201 gtgcgttttg atgcgcatca gcgcaccgtc cggctctggt acggtgaaat cgccattact 4261 accgcgaaag atgccctgca acgccccttc cgtgtcctga cccgtcaggg ccagctcact 4321 gctttaggga cagaatttac cgtccgccag caggataatt tcacgcagct tgacgtgcag 4381 cagcacgctg tggaagtgct tctcgccagt gcccccgcgc aaaaacgcat cgtgaacgct 4441 ggtgaaagcc tacagttcag cgcctctgag tttggcgcag tgaaaccgct ggatgacgag 4501 agtacaagct ggacgaagga catcctgagc ttcagcgata aaccgctggg tgaggtgata 4561 gccacgctaa cccgttaccg caacggcgtg ctgcgctgcg atcccgccgt tgccgggctg 4621 cgcctgagcg ggacgttccc gctgaaaaat accgatgcga tcctgaacgt tatcgcgcaa 4681 acgcttcccg ttaaaattca gtctattacg cggtactgga taaacatttc accactgtaa 4741 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 4801 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 4861 gcctttctgc gtttata //