##gff-version 3
##sequence-region BBa_J07085 1 4877
BBa_J07085	.	CDS	81	3200	.	+	0	ID=BBa_J07060;Name=BBa_J07060
BBa_J07085	.	sequence_feature	3209	3757	.	+	0	ID=BBa_J07068;Name=BBa_J07068
BBa_J07085	.	CDS	3230	3757	.	+	0	ID=BBa_J07022;Name=BBa_J07022;Parent=BBa_J07068
BBa_J07085	.	CDS	3230	3757	.	+	0	ID=annotation1577017;Name=FecI;Parent=BBa_J07022
BBa_J07085	.	ribosome_entry_site	3209	3223	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J07068
BBa_J07085	.	ribosome_entry_site	3216	3220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07085	.	ribosome_entry_site	3209	3223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J07085	.	engineered_region	3209	3223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07085	.	sequence_feature	3766	4877	.	+	0	ID=BBa_J07072;Name=BBa_J07072
BBa_J07085	.	ribosome_entry_site	3766	3780	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J07072
BBa_J07085	.	ribosome_entry_site	3773	3777	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07085	.	ribosome_entry_site	3766	3780	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J07085	.	engineered_region	3766	3780	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07085	.	terminator	4749	4828	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J07072
BBa_J07085	.	stem_loop	4760	4803	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07085	.	engineered_region	4749	4828	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07085	.	CDS	3787	4740	.	+	0	ID=BBa_J07023;Name=BBa_J07023;Parent=BBa_J07072
BBa_J07085	.	CDS	3787	4740	.	+	0	ID=annotation1577975;Name=FecR;Parent=BBa_J07023
BBa_J07085	.	terminator	4837	4877	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J07072
BBa_J07085	.	polyA_site	4864	4877	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07085	.	engineered_region	4837	4877	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07085	.	stem_loop	4844	4863	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07085	.	stop_codon	4870	4870	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07085	.	promoter	1	74	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_J07085	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_J07085	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07085	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_J07085	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07085	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07085	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07085	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07085	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_J07085
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgacgccgttacgcgtttttcgtaaaacaacacctttggttaacaccattcgcctgagcctgctgccgctggccggt
ctctcgttttccgcttttgctgcacaggttaatatcgcaccgggatcgctcgataaagcgctcaatcagtatgccgcac
acagcggatttaccctctcggttgacgccagcctgacgcgcggcaagcagagcaacggcctgcacggcgattacgacgt
cgagagcggcctgcaacaactgctggacggcagcggactgcaagtaaaaccgctgggaaataacagctggacgctggag
cccgcgcccgcaccaaaagaagatgccctgaccgtggtcggcgactggctgggtgatgcgcgtgaaaacgacgtatttg
aacatgctggcgcgcgtgacgtgatccgccgtgaggatttcgccaaaaccggcgcaaccaccatgcgtgaggtacttaa
ccgcatccctggcgtcagcgcgccggaaaacaacggcaccggcagccacgacctggcgatgaactttggcatccggggc
ctgaacccgcgcctcgccagccgctcgaccgtcctgatggacggcatccccgtccccttcgccccttacggtcagccgc
agctttcactggctcccgtttcgctcggcaacatggatgccattgacgtggtacgcggtggtggtgcggtgcgttacgg
accgcagagcgtgggcggcgtggtgaactttgttacccgtgccattccgcaggactttggtatcgaggcgggcgtggaa
ggtcagctcagcccaacctcttcacaaaacaacccgaaagagacgcacaacctgatggtgggcggcacagcggacaacg
gttttggcaccgcgctgctctactccggcacgcgcggcagtgactggcgcgagcacagcgccacccgcatcgacgacct
gatgctgaaaagcaaatatgcgccggatgaggtgcacaccttcaacagcctgctgcaatattacgacggtgaagccgac
atgcccggtggcctgtctcgcgcggattacgacgccgatcgctggcaatccacccgcccgtatgaccgcttctggggtc
gtcgcaagctggcgagcctgggctaccagttccagccagacagccagcataaattcaacattcaggggttctacaccca
aaccctgcgcagcggctacctggagcaaggcaaacgcatcaccctctcgccgcgtaactactgggtgcgcggtattgag
ccacgctacagccagatctttatgatcggcccttccgcgcacgaagtgggcgtgggctatcgctatttgaatgaatcaa
cgcatgaaatgcgttactacaccgccaccagcagcgggcagttgccgtccggctcaagcccttacgaccgcgatacgcg
ttccggcaccgaggcgcacgcctggtatctggatgacaaaatcgacatcggcaactggaccatcacgccgggtatgcgt
ttcgaacatatcgagtcataccagaacaacgccatcacaggcacgcacgaagaagtgagctataacgacgtcgttatga
ctcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtaca
cagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttcc
aaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtgg
aggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttga
aattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagat
ggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctg
gattcacttttggtcactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttag
aaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaa
agtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggta
tggaatacttaggtcaaggaacctcagtcaccgtctccccgaacagcgcgagccatagcggcagcgcgccgaacaccag
cagcgcgccgggcagctatcacctgactgacagctggaatctttatgcaaacactgaaggctcgttcggcaccgtacag
tacagccagattggcaaggctgtgcaaagcggcaatgttgaaccggaaaaagcgcgaacctgggaactcggtacccgct
acgacgacggcgcgctgacggcggaaatggggctgttcctgattaactttaacaatcagtacgactccaaccagaccaa
cgacaccgtcactgcacgtggcaaaacgcgccataccgggctggaaacgcaggcacgttacgatctgggtacgctaacg
ccaacgcttgataacgtttccatctacgccagctatgcgtatgtgaacgcggaaatccgcgagaaaggcgacacctacg
gcaatctggtaccattctccccgaaacataaaggcacgctgggcgtggactacaagccaggaaactggacgttcaatct
gaacagcgatttccagtccagccagtttgcggataacgccaatacggtgaaagagagcgccgacggcagtaccggccgc
attcccggcttcatgctctggggcgcacgcgtggcgtatgactttggcccgcagatggcagatctgaacctggcattcg
gtgtgaaaaacatcttcgaccaggactacttcatccgctcttatgacgacaacaacaaaggcatctatgcaggccagcc
gcgcacgctgtatatgcaggggtcgttgaagttctaataatactagagattaaagaggagaaatactagatgtctgacc
gcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcg
caaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcg
acgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctgg
aaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcga
gaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggat
ggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcg
agcactgcctgctgttccgtctggagtatgggttatgataataatactagagattaaagaggagaaatactagatgaat
cctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtca
gcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaa
cctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcac
gtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgc
gggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaataccca
aagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcg
aaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtcc
gccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaa
acgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagt
acaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgca
acggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgat
cctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaa
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata