LOCUS BBa_J07086 4874 bp DNA linear 17-May-2024 DEFINITION tetR promoter+rbs+scfv-FecA 567-576+rbs+FecI+rbs+FecR'+terminator ACCESSION BBa_J07086 VERSION BBa_J07086.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 promoter 1..74 /label=BBa_J13002 CDS 81..3197 /label=BBa_J07063 RBS 3206..3220 /label=RBS-1\Strong RBS 3213..3217 /label=RBS misc_feature 3206..3220 /label=BBa_B0030 RBS 3206..3220 /label=BBa_B0030 CDS 3227..3754 /label=FecI CDS 3227..3754 /label=BBa_J07022 misc_feature 3206..3754 /label=BBa_J07068 RBS 3763..3777 /label=RBS-1\Strong RBS 3770..3774 /label=RBS misc_feature 3763..3777 /label=BBa_B0030 RBS 3763..3777 /label=BBa_B0030 CDS 3784..4737 /label=FecR CDS 3784..4737 /label=BBa_J07023 stem_loop 4757..4800 /label=stem_loop misc_feature 4746..4825 /label=BBa_B0010 terminator 4746..4825 /label=BBa_B0010 stem_loop 4841..4860 /label=T7 TE misc_feature 4867..4867 /label=stop polyA_site 4861..4874 /label=polya misc_feature 4834..4874 /label=BBa_B0012 terminator 4834..4874 /label=BBa_B0012 misc_feature 3763..4874 /label=BBa_J07072 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg 121 ttaacaccat tcgcctgagc ctgctgccgc tggccggtct ctcgttttcc gcttttgctg 181 cacaggttaa tatcgcaccg ggatcgctcg ataaagcgct caatcagtat gccgcacaca 241 gcggatttac cctctcggtt gacgccagcc tgacgcgcgg caagcagagc aacggcctgc 301 acggcgatta cgacgtcgag agcggcctgc aacaactgct ggacggcagc ggactgcaag 361 taaaaccgct gggaaataac agctggacgc tggagcccgc gcccgcacca aaagaagatg 421 ccctgaccgt ggtcggcgac tggctgggtg atgcgcgtga aaacgacgta tttgaacatg 481 ctggcgcgcg tgacgtgatc cgccgtgagg atttcgccaa aaccggcgca accaccatgc 541 gtgaggtact taaccgcatc cctggcgtca gcgcgccgga aaacaacggc accggcagcc 601 acgacctggc gatgaacttt ggcatccggg gcctgaaccc gcgcctcgcc agccgctcga 661 ccgtcctgat ggacggcatc cccgtcccct tcgcccctta cggtcagccg cagctttcac 721 tggctcccgt ttcgctcggc aacatggatg ccattgacgt ggtacgcggt ggtggtgcgg 781 tgcgttacgg accgcagagc gtgggcggcg tggtgaactt tgttacccgt gccattccgc 841 aggactttgg tatcgaggcg ggcgtggaag gtcagctcag cccaacctct tcacaaaaca 901 acccgaaaga gacgcacaac ctgatggtgg gcggcacagc ggacaacggt tttggcaccg 961 cgctgctcta ctccggcacg cgcggcagtg actggcgcga gcacagcgcc acccgcatcg 1021 acgacctgat gctgaaaagc aaatatgcgc cggatgaggt gcacaccttc aacagcctgc 1081 tgcaatatta cgacggtgaa gccgacatgc ccggtggcct gtctcgcgcg gattacgacg 1141 ccgatcgctg gcaatccacc cgcccgtatg accgcttctg gggtcgtcgc aagctggcga 1201 gcctgggcta ccagttccag ccagacagcc agcataaatt caacattcag gggttctaca 1261 cccaaaccct gcgcagcggc tacctggagc aaggcaaacg catcaccctc tcgccgcgta 1321 actactgggt gcgcggtatt gagccacgct acagccagat ctttatgatc ggcccttccg 1381 cgcacgaagt gggcgtgggc tatcgctatt tgaatgaatc aacgcatgaa atgcgttact 1441 acaccgccac cagcagcggg cagttgccgt ccggctcaag cccttacgac cgcgatacgc 1501 gttccggcac cgaggcgcac gcctggtatc tggatgacaa aatcgacatc ggcaactgga 1561 ccatcacgcc gggtatgcgt ttcgaacata tcgagtcata ccagaacaac gccatcacag 1621 gcacgcacga agaagtgagc tataacgcac cgcttccggc gttgaacgtg ctctatcacc 1681 tgactgacag ctggaatctt tatgcaaaca ctgaaggctc gttcggcacc gtacagtaca 1741 gccagattgg caaggctgtg caaagcggca atgttgaaga cgtcgttatg actcaaacac 1801 cactatcact tcctgttagt ctaggtgatc aagcctccat ctcttgcaga tctagtcaga 1861 gccttgtaca cagtaatgga aacacctatt tacgttggta cctgcaaaag ccaggccagt 1921 ctccaaaggt cctgatctac aaagtttcca accgagtttc tggggtccca gacaggttca 1981 gtggcagtgg atcagggaca gatttcacac tcaagatcaa cagagtggag gctgaggatc 2041 tgggagttta tttctgctct caaagtacac atgttccgtg gacgttcggt ggaggcacca 2101 agcttgaaat taagtcctct gctgatgatg ctaagaagga tgctgctaag aaggatgatg 2161 ctaagaaaga tgatgctaag aaagatggtg gcgtcaaact ggatgagact ggaggaggct 2221 tggtgcaacc tgggggggcc atgaaactct cctgtgttac ctctggattc acttttggtc 2281 actactggat gaactgggtc cgccagtctc cagagaaagg actggagtgg gtagcacaat 2341 ttagaaacaa accttataat tatgaaacat attattcaga ttctgtgaaa ggcagattca 2401 ccatctcaag agatgattcc aaaagtagtg tctacctgca aatgaacaac ttaagagttg 2461 aagacaccgg tatctattac tgtacgggtg cgtcttatgg tatggaatac ttaggtcaag 2521 gaacctcagt caccgtctcc ccgaacagcg cgagccatag cggcagcgcg ccgaacacca 2581 gcagcgcgcc gggcagcacc cgctacgacg acggcgcgct gacggcggaa atggggctgt 2641 tcctgattaa ctttaacaat cagtacgact ccaaccagac caacgacacc gtcactgcac 2701 gtggcaaaac gcgccatacc gggctggaaa cgcaggcacg ttacgatctg ggtacgctaa 2761 cgccaacgct tgataacgtt tccatctacg ccagctatgc gtatgtgaac gcggaaatcc 2821 gcgagaaagg cgacacctac ggcaatctgg taccattctc cccgaaacat aaaggcacgc 2881 tgggcgtgga ctacaagcca ggaaactgga cgttcaatct gaacagcgat ttccagtcca 2941 gccagtttgc ggataacgcc aatacggtga aagagagcgc cgacggcagt accggccgca 3001 ttcccggctt catgctctgg ggcgcacgcg tggcgtatga ctttggcccg cagatggcag 3061 atctgaacct ggcattcggt gtgaaaaaca tcttcgacca ggactacttc atccgctctt 3121 atgacgacaa caacaaaggc atctatgcag gccagccgcg cacgctgtat atgcaggggt 3181 cgttgaagtt ctaataatac tagagattaa agaggagaaa tactagatgt ctgaccgcgc 3241 cactaccaca gcttccttaa cgttcgagtc gctttatggc acacatcacg gctggttgaa 3301 aagctggctg acgcgcaaac tccagtctgc ttttgatgca gatgacattg cccaggacac 3361 ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc cgcgatcctc gctccttcct 3421 ctgcactatc gccaaacgcg tgatggtgga cctgtttcgc cgaaacgcgc tggaaaaagc 3481 gtatctggag atgctggcgc ttatgccgga ggggggagcg ccttcacctg aggaacgcga 3541 aagccaactc gagaccctac aactcctcga cagcatgctg gacgggctaa acggcaaaac 3601 acgtgaagcg tttctgcttt cgcaactgga tggtctgaca tacagcgaga ttgcgcacaa 3661 actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg aaagccgtcg agcactgcct 3721 gctgttccgt ctggagtatg ggttatgata ataatactag agattaaaga ggagaaatac 3781 tagatgaatc ctttgttaac cgattcccgc cgtcaggcgc tgcgttcagc ttcccactgg 3841 tatgccgtgc taagcggcga gcgcgtcagc ccacaacagg aagcgcgctg gcaacagtgg 3901 tatgaacagg atcaggataa ccagtgggcc tggcagcagg ttgaaaacct gcgcaaccag 3961 cttggcggtg tgcctggcga cgttgccagc cgggcgttgc acgatacccg cctcacccgc 4021 cgtcacgtga tgaaaggatt actgctgttg ctcggcgctg gcggaggctg gcagctctgg 4081 cagtcggaaa ccggcgaagg tctgcgggca gattaccgca ccgccaaagg cacagtcagc 4141 cgtcagcaac tggaagatgg ctccctgctc acgctgaata cccaaagcgc ggcggatgtg 4201 cgttttgatg cgcatcagcg caccgtccgg ctctggtacg gtgaaatcgc cattactacc 4261 gcgaaagatg ccctgcaacg ccccttccgt gtcctgaccc gtcagggcca gctcactgct 4321 ttagggacag aatttaccgt ccgccagcag gataatttca cgcagcttga cgtgcagcag 4381 cacgctgtgg aagtgcttct cgccagtgcc cccgcgcaaa aacgcatcgt gaacgctggt 4441 gaaagcctac agttcagcgc ctctgagttt ggcgcagtga aaccgctgga tgacgagagt 4501 acaagctgga cgaaggacat cctgagcttc agcgataaac cgctgggtga ggtgatagcc 4561 acgctaaccc gttaccgcaa cggcgtgctg cgctgcgatc ccgccgttgc cgggctgcgc 4621 ctgagcggga cgttcccgct gaaaaatacc gatgcgatcc tgaacgttat cgcgcaaacg 4681 cttcccgtta aaattcagtc tattacgcgg tactggataa acatttcacc actgtaatac 4741 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 4801 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 4861 tttctgcgtt tata //