BBa_J07072 1 BBa_J07072 strong RBS+FecR'+terminator 2005-08-22T11:00:00Z 2015-08-31T04:08:20Z This part includes strong RBS (B0030), FecR' (J07023), and strong terminator (B0015). Built as an intermediate part of overall construct. false false _21_ 0 353 21 Not in stock false false Annie Vo component1665622 1 BBa_B0012 component1665607 1 BBa_J07023 component1665612 1 BBa_B0010 component1665601 1 BBa_B0030 annotation1665622 1 BBa_B0012 range1665622 1 1072 1112 annotation1665601 1 BBa_B0030 range1665601 1 1 15 annotation1665607 1 BBa_J07023 range1665607 1 22 975 annotation1665612 1 BBa_B0010 range1665612 1 984 1063 BBa_J13002 1 BBa_J13002 TetR repressed POPS/RIPS generator 2005-06-15T11:00:00Z 2015-08-31T04:08:29Z Released HQ 2013 -- No description -- false true _37_5_ 0 88 37 In stock false true Jeff Tabor component1535778 1 BBa_R0040 component1535786 1 BBa_B0034 annotation1535778 1 BBa_R0040 range1535778 1 1 54 annotation1535786 1 BBa_B0034 range1535786 1 63 74 BBa_J07023 1 BBa_J07023 FecR' 2005-07-13T11:00:00Z 2015-08-31T04:08:19Z MG1655 Wildtype FecR with pst1 (@664) from: ctgcag to ctacag false false _21_ 0 384 21 It's complicated false true jxyu annotation1577975 1 FecR range1577975 1 1 954 BBa_J07022 1 BBa_J07022 sigma factor FecI controls iron transport 2005-07-12T11:00:00Z 2015-08-31T04:08:19Z MG1655 FecI Coding sequence false false _21_ 0 379 21 It's complicated false false ymk annotation1577017 1 FecI range1577017 1 1 528 BBa_J07086 1 BBa_J07086 tetR promoter+rbs+scfv-FecA 567-576+rbs+FecI+rbs+FecR'+terminator 2005-09-27T11:00:00Z 2015-08-31T04:08:20Z This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. FecA' aa 567 to 576 are deleted and replaced by scFv. All are under control of tetR promoter. false false _21_ 0 353 21 Not in stock false false Annie Vo component2227217 1 BBa_J07068 component2227211 1 BBa_J07063 component2227229 1 BBa_J07072 component2227210 1 BBa_J13002 annotation2227211 1 BBa_J07063 range2227211 1 81 3197 annotation2227210 1 BBa_J13002 range2227210 1 1 74 annotation2227229 1 BBa_J07072 range2227229 1 3763 4874 annotation2227217 1 BBa_J07068 range2227217 1 3206 3754 BBa_J07063 1 BBa_J07063 FecA::scFv internal fusion type 2 @ aa566-577 2005-08-03T11:00:00Z 2015-08-31T04:08:20Z Internal fusion of anti-fluorescein antibody to FecA between aa566 and aa577 false false _21_ 0 379 21 Not in stock false false Yaser (Ray) Khan BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986785 1 -35 range1986785 1 20 25 BBa_J07068 1 BBa_J07068 RBS+FecI 2005-08-21T11:00:00Z 2015-08-31T04:08:20Z Part of system construction Includes strong RBS (B0030) and FecI (J07022) false false _21_ 0 353 21 Not in stock false false Annie Vo component2218191 1 BBa_J07022 component2218188 1 BBa_B0030 annotation2218191 1 BBa_J07022 range2218191 1 22 549 annotation2218188 1 BBa_B0030 range2218188 1 1 15 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J13002_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa BBa_J07023_sequence 1 atgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaa BBa_J07022_sequence 1 atgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_B0030_sequence 1 attaaagaggagaaa BBa_J07086_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgacgccgttacgcgtttttcgtaaaacaacacctttggttaacaccattcgcctgagcctgctgccgctggccggtctctcgttttccgcttttgctgcacaggttaatatcgcaccgggatcgctcgataaagcgctcaatcagtatgccgcacacagcggatttaccctctcggttgacgccagcctgacgcgcggcaagcagagcaacggcctgcacggcgattacgacgtcgagagcggcctgcaacaactgctggacggcagcggactgcaagtaaaaccgctgggaaataacagctggacgctggagcccgcgcccgcaccaaaagaagatgccctgaccgtggtcggcgactggctgggtgatgcgcgtgaaaacgacgtatttgaacatgctggcgcgcgtgacgtgatccgccgtgaggatttcgccaaaaccggcgcaaccaccatgcgtgaggtacttaaccgcatccctggcgtcagcgcgccggaaaacaacggcaccggcagccacgacctggcgatgaactttggcatccggggcctgaacccgcgcctcgccagccgctcgaccgtcctgatggacggcatccccgtccccttcgccccttacggtcagccgcagctttcactggctcccgtttcgctcggcaacatggatgccattgacgtggtacgcggtggtggtgcggtgcgttacggaccgcagagcgtgggcggcgtggtgaactttgttacccgtgccattccgcaggactttggtatcgaggcgggcgtggaaggtcagctcagcccaacctcttcacaaaacaacccgaaagagacgcacaacctgatggtgggcggcacagcggacaacggttttggcaccgcgctgctctactccggcacgcgcggcagtgactggcgcgagcacagcgccacccgcatcgacgacctgatgctgaaaagcaaatatgcgccggatgaggtgcacaccttcaacagcctgctgcaatattacgacggtgaagccgacatgcccggtggcctgtctcgcgcggattacgacgccgatcgctggcaatccacccgcccgtatgaccgcttctggggtcgtcgcaagctggcgagcctgggctaccagttccagccagacagccagcataaattcaacattcaggggttctacacccaaaccctgcgcagcggctacctggagcaaggcaaacgcatcaccctctcgccgcgtaactactgggtgcgcggtattgagccacgctacagccagatctttatgatcggcccttccgcgcacgaagtgggcgtgggctatcgctatttgaatgaatcaacgcatgaaatgcgttactacaccgccaccagcagcgggcagttgccgtccggctcaagcccttacgaccgcgatacgcgttccggcaccgaggcgcacgcctggtatctggatgacaaaatcgacatcggcaactggaccatcacgccgggtatgcgtttcgaacatatcgagtcataccagaacaacgccatcacaggcacgcacgaagaagtgagctataacgcaccgcttccggcgttgaacgtgctctatcacctgactgacagctggaatctttatgcaaacactgaaggctcgttcggcaccgtacagtacagccagattggcaaggctgtgcaaagcggcaatgttgaagacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctggattcacttttggtcactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggtatggaatacttaggtcaaggaacctcagtcaccgtctccccgaacagcgcgagccatagcggcagcgcgccgaacaccagcagcgcgccgggcagcacccgctacgacgacggcgcgctgacggcggaaatggggctgttcctgattaactttaacaatcagtacgactccaaccagaccaacgacaccgtcactgcacgtggcaaaacgcgccataccgggctggaaacgcaggcacgttacgatctgggtacgctaacgccaacgcttgataacgtttccatctacgccagctatgcgtatgtgaacgcggaaatccgcgagaaaggcgacacctacggcaatctggtaccattctccccgaaacataaaggcacgctgggcgtggactacaagccaggaaactggacgttcaatctgaacagcgatttccagtccagccagtttgcggataacgccaatacggtgaaagagagcgccgacggcagtaccggccgcattcccggcttcatgctctggggcgcacgcgtggcgtatgactttggcccgcagatggcagatctgaacctggcattcggtgtgaaaaacatcttcgaccaggactacttcatccgctcttatgacgacaacaacaaaggcatctatgcaggccagccgcgcacgctgtatatgcaggggtcgttgaagttctaataatactagagattaaagaggagaaatactagatgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataatactagagattaaagaggagaaatactagatgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J07068_sequence 1 attaaagaggagaaatactagatgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataa BBa_J07072_sequence 1 attaaagaggagaaatactagatgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J07063_sequence 1 atgacgccgttacgcgtttttcgtaaaacaacacctttggttaacaccattcgcctgagcctgctgccgctggccggtctctcgttttccgcttttgctgcacaggttaatatcgcaccgggatcgctcgataaagcgctcaatcagtatgccgcacacagcggatttaccctctcggttgacgccagcctgacgcgcggcaagcagagcaacggcctgcacggcgattacgacgtcgagagcggcctgcaacaactgctggacggcagcggactgcaagtaaaaccgctgggaaataacagctggacgctggagcccgcgcccgcaccaaaagaagatgccctgaccgtggtcggcgactggctgggtgatgcgcgtgaaaacgacgtatttgaacatgctggcgcgcgtgacgtgatccgccgtgaggatttcgccaaaaccggcgcaaccaccatgcgtgaggtacttaaccgcatccctggcgtcagcgcgccggaaaacaacggcaccggcagccacgacctggcgatgaactttggcatccggggcctgaacccgcgcctcgccagccgctcgaccgtcctgatggacggcatccccgtccccttcgccccttacggtcagccgcagctttcactggctcccgtttcgctcggcaacatggatgccattgacgtggtacgcggtggtggtgcggtgcgttacggaccgcagagcgtgggcggcgtggtgaactttgttacccgtgccattccgcaggactttggtatcgaggcgggcgtggaaggtcagctcagcccaacctcttcacaaaacaacccgaaagagacgcacaacctgatggtgggcggcacagcggacaacggttttggcaccgcgctgctctactccggcacgcgcggcagtgactggcgcgagcacagcgccacccgcatcgacgacctgatgctgaaaagcaaatatgcgccggatgaggtgcacaccttcaacagcctgctgcaatattacgacggtgaagccgacatgcccggtggcctgtctcgcgcggattacgacgccgatcgctggcaatccacccgcccgtatgaccgcttctggggtcgtcgcaagctggcgagcctgggctaccagttccagccagacagccagcataaattcaacattcaggggttctacacccaaaccctgcgcagcggctacctggagcaaggcaaacgcatcaccctctcgccgcgtaactactgggtgcgcggtattgagccacgctacagccagatctttatgatcggcccttccgcgcacgaagtgggcgtgggctatcgctatttgaatgaatcaacgcatgaaatgcgttactacaccgccaccagcagcgggcagttgccgtccggctcaagcccttacgaccgcgatacgcgttccggcaccgaggcgcacgcctggtatctggatgacaaaatcgacatcggcaactggaccatcacgccgggtatgcgtttcgaacatatcgagtcataccagaacaacgccatcacaggcacgcacgaagaagtgagctataacgcaccgcttccggcgttgaacgtgctctatcacctgactgacagctggaatctttatgcaaacactgaaggctcgttcggcaccgtacagtacagccagattggcaaggctgtgcaaagcggcaatgttgaagacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctggattcacttttggtcactactggatgaactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggtatggaatacttaggtcaaggaacctcagtcaccgtctccccgaacagcgcgagccatagcggcagcgcgccgaacaccagcagcgcgccgggcagcacccgctacgacgacggcgcgctgacggcggaaatggggctgttcctgattaactttaacaatcagtacgactccaaccagaccaacgacaccgtcactgcacgtggcaaaacgcgccataccgggctggaaacgcaggcacgttacgatctgggtacgctaacgccaacgcttgataacgtttccatctacgccagctatgcgtatgtgaacgcggaaatccgcgagaaaggcgacacctacggcaatctggtaccattctccccgaaacataaaggcacgctgggcgtggactacaagccaggaaactggacgttcaatctgaacagcgatttccagtccagccagtttgcggataacgccaatacggtgaaagagagcgccgacggcagtaccggccgcattcccggcttcatgctctggggcgcacgcgtggcgtatgactttggcccgcagatggcagatctgaacctggcattcggtgtgaaaaacatcttcgaccaggactacttcatccgctcttatgacgacaacaacaaaggcatctatgcaggccagccgcgcacgctgtatatgcaggggtcgttgaagttctaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z