BBa_J07086
type: ComponentDefinition
persistent identity: BBa J07086
display id: BBa_J07086
version: 1
was generated by: iGEM to SBOL conversion
description: tetR promoter+rbs+scfv-FecA 567-576+rbs+FecI+rbs+FecR'+terminator
created: 2005-09-27T11:00:00Z
modified: 2015-08-31T04:08:20Z
owned by: james
owned by: myers
mutable description: This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. FecA' aa 567 to 576 are deleted and replaced by scFv. All are under control of tetR promoter.
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Annie Vo
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J13002
component: BBa_J07068
component: BBa_J07063
component: BBa_J07072
sequence annotation: BBa_J13002
sequence annotation: BBa_J07063
sequence annotation: BBa_J07068
sequence annotation: BBa_J07072
sequence: https://synbiohub.org/public/igem/BBa_J07086_sequence/1

BBa_J13002
type: Component
persistent identity: component2227210
display id: component2227210
version: 1
top level: BBa_J07086
definition: BBa_J13002
access: public

BBa_J07068
type: Component
persistent identity: component2227217
display id: component2227217
version: 1
top level: BBa_J07086
definition: BBa_J07068
access: public

BBa_J07063
type: Component
persistent identity: component2227211
display id: component2227211
version: 1
top level: BBa_J07086
definition: BBa_J07063
access: public

BBa_J07072
type: Component
persistent identity: component2227229
display id: component2227229
version: 1
top level: BBa_J07086
definition: BBa_J07072
access: public

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2227210
display id: annotation2227210
version: 1
top level: BBa_J07086
location: https://synbiohub.org/public/igem/BBa_J07086/annotation2227210/range2227210/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_J07086/annotation2227210/range2227210/1
type: Range
persistent identity: range2227210
display id: range2227210
version: 1
top level: BBa_J07086
direction: direction/reverse
start: 1
end: 74
orientation: inline

BBa_J07063
type: SequenceAnnotation
persistent identity: annotation2227211
display id: annotation2227211
version: 1
top level: BBa_J07086
location: https://synbiohub.org/public/igem/BBa_J07086/annotation2227211/range2227211/1
component: BBa_J07063

https://synbiohub.org/public/igem/BBa_J07086/annotation2227211/range2227211/1
type: Range
persistent identity: range2227211
display id: range2227211
version: 1
top level: BBa_J07086
direction: direction/reverse
start: 81
end: 3197
orientation: inline

BBa_J07068
type: SequenceAnnotation
persistent identity: annotation2227217
display id: annotation2227217
version: 1
top level: BBa_J07086
location: https://synbiohub.org/public/igem/BBa_J07086/annotation2227217/range2227217/1
component: BBa_J07068

https://synbiohub.org/public/igem/BBa_J07086/annotation2227217/range2227217/1
type: Range
persistent identity: range2227217
display id: range2227217
version: 1
top level: BBa_J07086
direction: direction/reverse
start: 3206
end: 3754
orientation: inline

BBa_J07072
type: SequenceAnnotation
persistent identity: annotation2227229
display id: annotation2227229
version: 1
top level: BBa_J07086
location: https://synbiohub.org/public/igem/BBa_J07086/annotation2227229/range2227229/1
component: BBa_J07072

https://synbiohub.org/public/igem/BBa_J07086/annotation2227229/range2227229/1
type: Range
persistent identity: range2227229
display id: range2227229
version: 1
top level: BBa_J07086
direction: direction/reverse
start: 3763
end: 4874
orientation: inline

BBa_J07063
type: ComponentDefinition
persistent identity: BBa J07063
display id: BBa_J07063
version: 1
was generated by: iGEM to SBOL conversion
description: FecA::scFv internal fusion type 2 @ aa566-577
created: 2005-08-03T11:00:00Z
modified: 2015-08-31T04:08:20Z
owned by: james
owned by: myers
mutable description: Internal fusion of anti-fluorescein antibody to FecA between aa566 and aa577
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 379
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yaser (Ray) Khan
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_J07063_sequence/1

BBa_J07068
type: ComponentDefinition
persistent identity: BBa J07068
display id: BBa_J07068
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+FecI
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:08:20Z
owned by: james
owned by: myers
mutable description: Part of system construction Includes strong RBS (B0030) and FecI (J07022)
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Annie Vo
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_J07022
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_J07022
sequence: https://synbiohub.org/public/igem/BBa_J07068_sequence/1

BBa_J07022
type: Component
persistent identity: component2218191
display id: component2218191
version: 1
top level: BBa_J07068
definition: BBa_J07022
access: public

BBa_B0030
type: Component
persistent identity: component2218188
display id: component2218188
version: 1
top level: BBa_J07068
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2218188
display id: annotation2218188
version: 1
top level: BBa_J07068
location: https://synbiohub.org/public/igem/BBa_J07068/annotation2218188/range2218188/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J07068/annotation2218188/range2218188/1
type: Range
persistent identity: range2218188
display id: range2218188
version: 1
top level: BBa_J07068
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_J07022
type: SequenceAnnotation
persistent identity: annotation2218191
display id: annotation2218191
version: 1
top level: BBa_J07068
location: https://synbiohub.org/public/igem/BBa_J07068/annotation2218191/range2218191/1
component: BBa_J07022

https://synbiohub.org/public/igem/BBa_J07068/annotation2218191/range2218191/1
type: Range
persistent identity: range2218191
display id: range2218191
version: 1
top level: BBa_J07068
direction: direction/reverse
start: 22
end: 549
orientation: inline

BBa_J07072
type: ComponentDefinition
persistent identity: BBa J07072
display id: BBa_J07072
version: 1
was generated by: iGEM to SBOL conversion
description: strong RBS+FecR'+terminator
created: 2005-08-22T11:00:00Z
modified: 2015-08-31T04:08:20Z
owned by: james
owned by: myers
mutable description: This part includes strong RBS (B0030), FecR' (J07023), and strong terminator (B0015). Built as an intermediate part of overall construct.
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Annie Vo
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_B0012
component: BBa_B0010
component: BBa_J07023
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_J07023
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J07072_sequence/1

BBa_B0012
type: Component
persistent identity: component1665622
display id: component1665622
version: 1
top level: BBa_J07072
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1665612
display id: component1665612
version: 1
top level: BBa_J07072
definition: BBa_B0010
access: public

BBa_J07023
type: Component
persistent identity: component1665607
display id: component1665607
version: 1
top level: BBa_J07072
definition: BBa_J07023
access: public

BBa_B0030
type: Component
persistent identity: component1665601
display id: component1665601
version: 1
top level: BBa_J07072
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1665601
display id: annotation1665601
version: 1
top level: BBa_J07072
location: https://synbiohub.org/public/igem/BBa_J07072/annotation1665601/range1665601/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J07072/annotation1665601/range1665601/1
type: Range
persistent identity: range1665601
display id: range1665601
version: 1
top level: BBa_J07072
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_J07023
type: SequenceAnnotation
persistent identity: annotation1665607
display id: annotation1665607
version: 1
top level: BBa_J07072
location: https://synbiohub.org/public/igem/BBa_J07072/annotation1665607/range1665607/1
component: BBa_J07023

https://synbiohub.org/public/igem/BBa_J07072/annotation1665607/range1665607/1
type: Range
persistent identity: range1665607
display id: range1665607
version: 1
top level: BBa_J07072
direction: direction/forward
start: 22
end: 975
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1665612
display id: annotation1665612
version: 1
top level: BBa_J07072
location: https://synbiohub.org/public/igem/BBa_J07072/annotation1665612/range1665612/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07072/annotation1665612/range1665612/1
type: Range
persistent identity: range1665612
display id: range1665612
version: 1
top level: BBa_J07072
direction: direction/forward
start: 984
end: 1063
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1665622
display id: annotation1665622
version: 1
top level: BBa_J07072
location: https://synbiohub.org/public/igem/BBa_J07072/annotation1665622/range1665622/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07072/annotation1665622/range1665622/1
type: Range
persistent identity: range1665622
display id: range1665622
version: 1
top level: BBa_J07072
direction: direction/forward
start: 1072
end: 1112
orientation: inline

BBa_J13002
type: ComponentDefinition
persistent identity: BBa J13002
display id: BBa_J13002
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressed POPS/RIPS generator
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13002_sequence/1

BBa_B0034
type: Component
persistent identity: component1535786
display id: component1535786
version: 1
top level: BBa_J13002
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1535778
display id: component1535778
version: 1
top level: BBa_J13002
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1535778
display id: annotation1535778
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
type: Range
persistent identity: range1535778
display id: range1535778
version: 1
top level: BBa_J13002
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1535786
display id: annotation1535786
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
type: Range
persistent identity: range1535786
display id: range1535786
version: 1
top level: BBa_J13002
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_J07022
type: ComponentDefinition
persistent identity: BBa J07022
display id: BBa_J07022
version: 1
was generated by: iGEM to SBOL conversion
description: sigma factor FecI controls iron transport
created: 2005-07-12T11:00:00Z
modified: 2015-08-31T04:08:19Z
mutable provenance: MG1655
owned by: james
owned by: myers
mutable description: FecI Coding sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 379
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: ymk
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FecI
sequence: https://synbiohub.org/public/igem/BBa_J07022_sequence/1

FecI
type: SequenceAnnotation
persistent identity: annotation1577017
display id: annotation1577017
version: 1
top level: BBa_J07022
location: https://synbiohub.org/public/igem/BBa_J07022/annotation1577017/range1577017/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J07022/annotation1577017/range1577017/1
type: Range
persistent identity: range1577017
display id: range1577017
version: 1
top level: BBa_J07022
direction: direction/forward
start: 1
end: 528
orientation: inline

BBa_J07023
type: ComponentDefinition
persistent identity: BBa J07023
display id: BBa_J07023
version: 1
was generated by: iGEM to SBOL conversion
description: FecR'
created: 2005-07-13T11:00:00Z
modified: 2015-08-31T04:08:19Z
mutable provenance: MG1655
owned by: james
owned by: myers
mutable description: Wildtype FecR with pst1 (@664) from: ctgcag to ctacag
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 384
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
star: true
creator: jxyu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FecR
sequence: https://synbiohub.org/public/igem/BBa_J07023_sequence/1

FecR
type: SequenceAnnotation
persistent identity: annotation1577975
display id: annotation1577975
version: 1
top level: BBa_J07023
location: https://synbiohub.org/public/igem/BBa_J07023/annotation1577975/range1577975/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J07023/annotation1577975/range1577975/1
type: Range
persistent identity: range1577975
display id: range1577975
version: 1
top level: BBa_J07023
direction: direction/forward
start: 1
end: 954
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

https://synbiohub.org/public/igem/BBa_J07086_sequence/1
type: Sequence
persistent identity: BBa J07086 sequence
display id: BBa_J07086_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgacgccgttacgcgtttttcgtaaaacaacacctttggttaacaccattcgcctgagcctgctgccgctggccggtctctcgttttccgcttttgctgcacaggttaatatcgcaccgggatcgctcgataaagcgctcaatcagtatgccgcacacagcggatttaccctctcggttgacgccagcctgacgcgcggcaagcagagcaacggcctgcacggcgattacgacgtcgagagcggcctgcaacaactgctggacggcagcggactgcaagtaaaaccgctgggaaataacagctggacgctggagcccgcgcccgcaccaaaagaagatgccctgaccgtggtcggcgactggctgggtgatgcgcgtgaaaacgacgtatttgaacatgctggcgcgcgtgacgtgatccgccgtgaggatttcgccaaaaccggcgcaaccaccatgcgtgaggtacttaaccgcatccctggcgtcagcgcgccggaaaacaacggcaccggcagccacgacctggcgatgaactttggcatccggggcctgaacccgcgcctcgccagccgctcgaccgtcctgatggacggcatccccgtccccttcgccccttacggtcagccgcagctttcactggctcccgtttcgctcggcaacatggatgccattgacgtggtacgcggtggtggtgcggtgcgttacggaccgcagagcgtgggcggcgtggtgaactttgttacccgtgccattccgcaggactttggtatcgaggcgggcgtggaaggtcagctcagcccaacctcttcacaaaacaacccgaaagagacgcacaacctgatggtgggcggcacagcggacaacggttttggcaccgcgctgctctactccggcacgcgcggcagtgactggcgcgagcacagcgccacccgcatcgacgacctgatgctgaaaagcaaatatgcgccggatgaggtgcacaccttcaacagcctgctgcaatattacgacggtgaagccgacatgcccggtggcctgtctcgcgcggattacgacgccgatcgctggcaatccacccgcccgtatgaccgcttctggggtcgtcgcaagctggcgagcctgggctaccagttccagccagacagccagcataaattcaacattcaggggttctacacccaaaccctgcgcagcggctacctggagcaaggcaaacgcatcaccctctcgccgcgtaactactgggtgcgcggtattgagccacgctacagccagatctttatgatcggcccttccgcgcacgaagtgggcgtgggctatcgctatttgaatgaatcaacgcatgaaatgcgttactacaccgccaccagcagcgggcagttgccgtccggctcaagcccttacgaccgcgatacgcgttccggcaccgaggcgcacgcctggtatctggatgacaaaatcgacatcggcaactggaccatcacgccgggtatgcgtttcgaacatatcgagtcataccagaacaacgccatcacaggcacgcacgaagaagtgagctataacgcaccgcttccggcgttgaacgtgctctatcacctgactgacagctggaatctttatgcaaacactgaaggctcgttcggcaccgtacagtacagccagattggcaaggctgtgcaaagcggcaatgttgaagacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07063_sequence/1
type: Sequence
persistent identity: BBa J07063 sequence
display id: BBa_J07063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacgccgttacgcgtttttcgtaaaacaacacctttggttaacaccattcgcctgagcctgctgccgctggccggtctctcgttttccgcttttgctgcacaggttaatatcgcaccgggatcgctcgataaagcgctcaatcagtatgccgcacacagcggatttaccctctcggttgacgccagcctgacgcgcggcaagcagagcaacggcctgcacggcgattacgacgtcgagagcggcctgcaacaactgctggacggcagcggactgcaagtaaaaccgctgggaaataacagctggacgctggagcccgcgcccgcaccaaaagaagatgccctgaccgtggtcggcgactggctgggtgatgcgcgtgaaaacgacgtatttgaacatgctggcgcgcgtgacgtgatccgccgtgaggatttcgccaaaaccggcgcaaccaccatgcgtgaggtacttaaccgcatccctggcgtcagcgcgccggaaaacaacggcaccggcagccacgacctggcgatgaactttggcatccggggcctgaacccgcgcctcgccagccgctcgaccgtcctgatggacggcatccccgtccccttcgccccttacggtcagccgcagctttcactggctcccgtttcgctcggcaacatggatgccattgacgtggtacgcggtggtggtgcggtgcgttacggaccgcagagcgtgggcggcgtggtgaactttgttacccgtgccattccgcaggactttggtatcgaggcgggcgtggaaggtcagctcagcccaacctcttcacaaaacaacccgaaagagacgcacaacctgatggtgggcggcacagcggacaacggttttggcaccgcgctgctctactccggcacgcgcggcagtgactggcgcgagcacagcgccacccgcatcgacgacctgatgctgaaaagcaaatatgcgccggatgaggtgcacaccttcaacagcctgctgcaatattacgacggtgaagccgacatgcccggtggcctgtctcgcgcggattacgacgccgatcgctggcaatccacccgcccgtatgaccgcttctggggtcgtcgcaagctggcgagcctgggctaccagttccagccagacagccagcataaattcaacattcaggggttctacacccaaaccctgcgcagcggctacctggagcaaggcaaacgcatcaccctctcgccgcgtaactactgggtgcgcggtattgagccacgctacagccagatctttatgatcggcccttccgcgcacgaagtgggcgtgggctatcgctatttgaatgaatcaacgcatgaaatgcgttactacaccgccaccagcagcgggcagttgccgtccggctcaagcccttacgaccgcgatacgcgttccggcaccgaggcgcacgcctggtatctggatgacaaaatcgacatcggcaactggaccatcacgccgggtatgcgtttcgaacatatcgagtcataccagaacaacgccatcacaggcacgcacgaagaagtgagctataacgcaccgcttccggcgttgaacgtgctctatcacctgactgacagctggaatctttatgcaaacactgaaggctcgttcggcaccgtacagtacagccagattggcaaggctgtgcaaagcggcaatgttgaagacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07068_sequence/1
type: Sequence
persistent identity: BBa J07068 sequence
display id: BBa_J07068_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07072_sequence/1
type: Sequence
persistent identity: BBa J07072 sequence
display id: BBa_J07072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J13002_sequence/1
type: Sequence
persistent identity: BBa J13002 sequence
display id: BBa_J13002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07022_sequence/1
type: Sequence
persistent identity: BBa J07022 sequence
display id: BBa_J07022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgaccgcgccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaaactccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacgatccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaaaagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagaccctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggtctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagcactgcctgctgttccgtctggagtatgggttatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J07023_sequence/1
type: Sequence
persistent identity: BBa J07023 sequence
display id: BBa_J07023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatcctttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcccacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacctgcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtgatgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcgggcagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaagcgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaagatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgccagcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacgcatcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtacaagctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacggcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcctgaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z