LOCUS       BBa_J09034               920 bp    DNA     linear       22-Dec-2024
DEFINITION  EYFP AAV+ device with strong RBS
ACCESSION   BBa_J09034
VERSION     BBa_J09034.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    778..783
                     /label=stop
     misc_feature    739..777
                     /label=AAV
     misc_feature    22..783
                     /label=BBa_E0034
     stem_loop       803..846
                     /label=stem_loop
     misc_feature    792..871
                     /label=BBa_B0010
     terminator      792..871
                     /label=BBa_B0010
     stem_loop       887..906
                     /label=T7 TE
     misc_feature    913..913
                     /label=stop
     polyA_site      907..920
                     /label=polya
     misc_feature    880..920
                     /label=BBa_B0012
     terminator      880..920
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
      241 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      481 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      541 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      601 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      721 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgctgc tgctgtttaa
      781 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      901 tgggcctttc tgcgtttata
//