BBa_J09271 1 BBa_J09271 motB flagellar protein, without endogenous RBS 2006-10-25T11:00:00Z 2015-08-31T04:08:21Z This part was amplified by PCR from the genome of E. coli strain RP437 (wild-type for motility) Released HQ 2013 motB E. coli flagellar protein linking torque machinery to cell wall; allows for induction of motility false true _72_40_ 0 475 72 In stock true We had to design this part to address leaky expression from R0062. In order to induce motility with HSL, we must be able to demonstrate both repression and induction. S03271 contains the endogenous RBS of motB and is thought to be strong. Leaky expression could be diminished by adding weaker ribosome binding sites upstream of this part. true Jon Badalamenti BBa_J09271_sequence 1 atgaagaatcaagcgcatccgattattgtcgtcaaacgacgcaaagccaaaagccacggggcagcacatggatcgtggaagattgcttatgccgactttatgactgcgatgatggccttttttctggtgatgtggctgatctccatctccagcccaaaagagctgattcagattgcggagtacttccggactccactggcgactgcggttacgggcggcgatcgcatttctaatagtgaaagcccaattcccggcggtggtgatgattacacccaaagccagggggaagtgaataagcagccgaacatcgaagagctgaaaaaacgcatggagcaaagtcgattgcggaaattgcggggtgatctcgaccagttgatagagtccgatccgaaactgcgggcgttacgtccccatctcaaaatcgatctggtccaggaaggtctacgtattcagatcatcgatagccagaatcgcccgatgtttagaaccggcagtgccgatgtcgaaccctatatgcgcgacattctgcgcgccattgcgcctgtactgaacggtattcccaaccgtattagcctttcaggtcataccgatgatttcccctacgccagcggtgagaaaggatatagcaactgggagctttctgccgatcgggccaatgcatcccgccgcgaactgatggtcggagggttggatagcggcaaagtgttacgtgtcgtcggcatggcggcaacgatgcgcttaagcgatcgcggacctgatgatgccgtcaaccgtcgcatcagcctgctggtactgaacaaacaagccgaacaggccattttgcatgaaaacgccgaaagccagaatgagccagtaagcgccctggaaaaacctgaggttgcaccacaggtcagtgttcccacaatgccatcagccgaaccgaggtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z