LOCUS       BBa_J09342              2363 bp    DNA     linear       18-May-2024
DEFINITION  HSL->lacI
ACCESSION   BBa_J09342
VERSION     BBa_J09342.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..65
                     /label=prefix
     misc_feature    237..237
                     /label=T
     misc_feature    555..555
                     /label=A
     CDS             64..813
                     /label=luxR
     misc_feature    64..819
                     /label=BBa_C0062
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             64..819
                     /label=BBa_C0062
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     misc_feature    46..981
                     /label=BBa_I0462
     misc_feature    990..1009
                     /label=LuxR/HSL
     promoter        1009..1014
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1042..1042
                     /label=start
     misc_feature    990..1044
                     /label=BBa_R0062
     promoter        990..1044
                     /label=BBa_R0062
     misc_feature    1..1044
                     /label=BBa_J09855
     RBS             1053..1067
                     /label=RBS-1\Strong
     RBS             1060..1064
                     /label=RBS
     misc_feature    1053..1067
                     /label=BBa_B0030
     RBS             1053..1067
                     /label=BBa_B0030
     misc_feature    2163..2201
                     /label=LVA
     CDS             1074..2201
                     /label=lacI-LVA
     misc_feature    1074..2201
                     /label=BBa_C0012
     misc_feature    2202..2226
                     /label=Help:Barcodes
     CDS             1074..2201
                     /label=BBa_C0012
     stem_loop       2246..2289
                     /label=stem_loop
     misc_feature    2235..2314
                     /label=BBa_B0010
     terminator      2235..2314
                     /label=BBa_B0010
     stem_loop       2330..2349
                     /label=T7 TE
     misc_feature    2356..2356
                     /label=stop
     polyA_site      2350..2363
                     /label=polya
     misc_feature    2323..2363
                     /label=BBa_B0012
     terminator      2323..2363
                     /label=BBa_B0012
     terminator      2235..2363
                     /label=BBa_B0015
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag agattaaaga ggagaaatac tagatggtga
     1081 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     1141 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     1201 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
     1261 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
     1321 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
     1381 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
     1441 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
     1501 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
     1561 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
     1621 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
     1681 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
     1741 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
     1801 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
     1861 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
     1921 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca
     1981 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     2041 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
     2101 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2161 aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta
     2221 gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2281 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     2341 gggtgggcct ttctgcgttt ata
//