BBa_K199002
1
BBa_K199002
CCACU tRNA Suppressor (Produces Serine)
2009-06-25T11:00:00Z
2015-05-08T01:11:18Z
De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
This gene encodes for a 5 base pair tRNA suppressor that suppresses CCACU, which would normally cause a frame shift mutation.
false
false
_295_
0
5109
9
It's complicated
false
We used the 9 base anticodon loop that is complementary to the 5 base pair codon CCACU.
false
Shamita Punjabi
annotation2006708
1
Anticodon Loop
range2006708
1
44
52
annotation2006707
1
5' Context
range2006707
1
1
11
annotation2006709
1
3' Context
range2006709
1
104
143
BBa_S04333
1
BBa_S04333
K199106:K199002
2009-11-18T12:00:00Z
2015-05-08T01:14:38Z
false
false
_9_
0
5562
9
Not in stock
false
false
Mary Gearing
component2062779
1
BBa_K199106
component2062783
1
BBa_K199002
annotation2062783
1
BBa_K199002
range2062783
1
58
200
annotation2062779
1
BBa_K199106
range2062779
1
1
49
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_J100001
1
BBa_J100001
pTet+RBS+Cre2SAT1Clause+pLpp+tRNA CCACU
2011-05-23T11:00:00Z
2015-08-31T04:08:21Z
N/A
Suppressor tRNA CCACU (K199002) is produced under regulation by the lpp promoter (K199106). The tRNA suppresses one of the frameshift mutations in Cre, restoring its function.
false
false
_578_
0
5111
9
Not in stock
false
N/A
false
Eric Sawyer
component2120408
1
BBa_J100000
component2120414
1
BBa_S04333
component2120402
1
BBa_B0034
component2120396
1
BBa_R0040
annotation2120414
1
BBa_S04333
range2120414
1
1158
1357
annotation2120402
1
BBa_B0034
range2120402
1
63
74
annotation2120408
1
BBa_J100000
range2120408
1
81
1149
annotation2120396
1
BBa_R0040
range2120396
1
1
54
BBa_K199106
1
BBa_K199106
pLpp promoter
2009-11-18T12:00:00Z
2015-05-08T01:11:20Z
The sequence matches that naturally found in E. coli.
The lpp promoter is one of the strongest promoters in E. coli.
false
false
_295_
0
5562
9
Not in stock
false
n/a
false
Mary Gearing
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J100000
1
BBa_J100000
Cre with 8bp restriction sites and 1-Clause 2-SAT Problem Inserted
2011-05-23T11:00:00Z
2015-08-31T04:08:21Z
N/A
Cre recombines DNA at lox sites (J61046). After the start codon is an AsiSI site, followed by a SAT problem, followed by an AscI site. If the tRNA K199002 OR the tRNA K199001 is expressed in the cell, then Cre is read in-frame. If neither tRNA is expressed, then Cre is frameshifted.
false
false
_578_
0
5111
9
Not in stock
false
N/A
false
Eric Sawyer
annotation2120391
1
Start
range2120391
1
1
3
annotation2120395
1
Remainder of Cre Recombinase
range2120395
1
36
1069
annotation2120392
1
AsiSI site
range2120392
1
4
11
annotation2120393
1
1 Clause 2-SAT
range2120393
1
13
23
annotation2120394
1
AscI site
range2120394
1
28
35
BBa_K199002_sequence
1
ggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_S04333_sequence
1
atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_K199106_sequence
1
atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacg
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_J100000_sequence
1
atggcgatcgcaccactgctagttctgggcgcgcctccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatt
BBa_J100001_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcgatcgcaccactgctagttctgggcgcgcctccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatttactagagatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z