LOCUS       BBa_J100007              533 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac+RBS+LoxP+Stop Sequence+LoxP
ACCESSION   BBa_J100007
VERSION     BBa_J100007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    229..262
                     /label=BBa_J61046
     stem_loop       271..491
                     /label=Stem-loop
     misc_feature    271..491
                     /label=BBa_J100005
     misc_feature    500..533
                     /label=BBa_J61046
     misc_feature    229..533
                     /label=BBa_J100006
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagat aacttcgtat
      241 aatgtatgct atacgaagtt attactagag aaggcctgat tatgaagagc gtgtgacaga
      301 gacacataga atatctcaca cgacatggca gttaaataat aaaaaagccg gattaataat
      361 ctggcttttt attattctct ctctagtata taaacgcaga aaggcccacc cgaaggtgag
      421 ccagcgtgaa ctgccaatct gggttaagtt atcttaggtt ttctgcaccg cgccttcttt
      481 aatcaggcct ttactagaga taacttcgta taatgtatgc tatacgaagt tat
//