BBa_K199002
1
BBa_K199002
CCACU tRNA Suppressor (Produces Serine)
2009-06-25T11:00:00Z
2015-05-08T01:11:18Z
De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
This gene encodes for a 5 base pair tRNA suppressor that suppresses CCACU, which would normally cause a frame shift mutation.
false
false
_295_
0
5109
9
It's complicated
false
We used the 9 base anticodon loop that is complementary to the 5 base pair codon CCACU.
false
Shamita Punjabi
annotation2006708
1
Anticodon Loop
range2006708
1
44
52
annotation2006707
1
5' Context
range2006707
1
1
11
annotation2006709
1
3' Context
range2006709
1
104
143
BBa_K199106
1
BBa_K199106
pLpp promoter
2009-11-18T12:00:00Z
2015-05-08T01:11:20Z
The sequence matches that naturally found in E. coli.
The lpp promoter is one of the strongest promoters in E. coli.
false
false
_295_
0
5562
9
Not in stock
false
n/a
false
Mary Gearing
BBa_J100009
1
BBa_J100009
pLpp-tRNA CCACU-pLpp-tRNA CGGUC
2011-06-05T11:00:00Z
2015-08-31T04:08:21Z
N/A
Produces suppressor tRNA CCACU (K199002) and suppressor tRNA CGGUC (K199028), each regulated by the lpp promoter (K199106).
false
false
_578_
0
5111
9
Not in stock
false
N/A
false
Eric Sawyer
component2120755
1
BBa_S04334
component2120749
1
BBa_S04333
annotation2120755
1
BBa_S04334
range2120755
1
209
408
annotation2120749
1
BBa_S04333
range2120749
1
1
200
BBa_S04333
1
BBa_S04333
K199106:K199002
2009-11-18T12:00:00Z
2015-05-08T01:14:38Z
false
false
_9_
0
5562
9
Not in stock
false
false
Mary Gearing
component2062783
1
BBa_K199002
component2062779
1
BBa_K199106
annotation2062783
1
BBa_K199002
range2062783
1
58
200
annotation2062779
1
BBa_K199106
range2062779
1
1
49
BBa_S04334
1
BBa_S04334
K199106:K199028
2009-11-18T12:00:00Z
2015-05-08T01:14:38Z
false
false
_9_
0
5562
9
Not in stock
false
false
Mary Gearing
component2062788
1
BBa_K199028
component2062784
1
BBa_K199106
annotation2062788
1
BBa_K199028
range2062788
1
58
200
annotation2062784
1
BBa_K199106
range2062784
1
1
49
BBa_K199028
1
BBa_K199028
CGGUC tRNA Suppressor (Produces Serine)
2009-07-08T11:00:00Z
2015-05-08T01:11:18Z
De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
This gene encodes for a 5 base pair tRNA suppressor that suppresses CGGUC, which would normally cause a frame shift mutation.
false
false
_295_
0
5117
9
It's complicated
false
We used the 9 base anticodon loop that is complementary to the 5 base pair codon CGGUC.
false
Alyndria Thompson
annotation2010430
1
anticodon loop
range2010430
1
44
52
annotation2010432
1
3
range2010432
1
104
143
annotation2010431
1
5' context
range2010431
1
1
11
BBa_K199002_sequence
1
ggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_K199028_sequence
1
ggatccaattcggagagatgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_S04333_sequence
1
atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_K199106_sequence
1
atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacg
BBa_S04334_sequence
1
atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_J100009_sequence
1
atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z