LOCUS BBa_J100015 2757 bp DNA linear 22-Dec-2024 DEFINITION 1 Clause 2-SAT Problem with Frameshifted LuxI and a GFP Reporter ACCESSION BBa_J100015 VERSION BBa_J100015.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 misc_feature 219..221 /label=Start misc_feature 222..229 /label=AsiSI Site misc_feature 231..241 /label=SAT Problem misc_feature 246..253 /label=AscI Site misc_feature 851..856 /label=Stop CDS 219..856 /label=BBa_J100013 RBS 865..879 /label=RBS-1\Strong RBS 872..876 /label=RBS misc_feature 865..879 /label=BBa_B0030 RBS 865..879 /label=BBa_B0030 CDS 886..1605 /label=GFP protein CDS 886..1605 /label=BBa_E0040 CDS 1614..1701 /label=end of LacI coding region (inactive) misc_feature 1614..1813 /label=BBa_R0010 misc_feature 1702..1739 /label=CAP binding site promoter 1750..1755 /label=-35 promoter 1774..1779 /label=-10 misc_feature 1779..1813 /label=LacI binding site misc_feature 1786..1786 /label=start promoter 1614..1813 /label=BBa_R0010 misc_feature 1826..1829 /label=conserved RBS 1822..1833 /label=BBa_B0034 misc_feature 1840..1841 /label=prefix misc_feature 2013..2013 /label=T misc_feature 2331..2331 /label=A CDS 1840..2589 /label=luxR misc_feature 1840..2595 /label=BBa_C0062 misc_feature 2596..2620 /label=Help:Barcodes CDS 1840..2595 /label=BBa_C0062 stem_loop 2640..2683 /label=stem_loop misc_feature 2629..2708 /label=BBa_B0010 terminator 2629..2708 /label=BBa_B0010 stem_loop 2724..2743 /label=T7 TE misc_feature 2750..2750 /label=stop polyA_site 2744..2757 /label=polya misc_feature 2717..2757 /label=BBa_B0012 terminator 2717..2757 /label=BBa_B0012 terminator 2629..2757 /label=BBa_B0015 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgca ccactgctag 241 ttctgggcgc gccgcaattg caagcggtcg tgcaaccatt atgattaaaa aaagcgattt 301 tctggccatt ccgagcgaag aatataaagg tattctgagc ctgcgctatc aggtttttaa 361 acagcgtctg gaatgggatc tggtggttga aaataatctg gaaagtgatg aatatgataa 421 tagcaatgcc gaatatattt atgcctgtga tgataccgaa aatgttagcg gttgttggcg 481 tctgctgccg accaccggtg attatatgct gaaaagcgtt tttccggaac tgctgggtca 541 gcagagcgca ccgaaagatc cgaatattgt tgaactgagc cgttttgccg tgggtaaaaa 601 tagcagcaaa attaataata gcgccagcga aattaccatg aaactgtttg aagccattta 661 taaacatgcc gttagccagg gtattaccga atatgttacc gttaccagca ccgcaattga 721 acgttttctg aaacgtatta aagtgccgtg tcatcgtatt ggcgataaag aaattcatgt 781 tctgggcgat accaaaagcg ttgttctgag catgccgatt aatgaacagt ttaaaaaagc 841 cgtgctgaac taataatact agagattaaa gaggagaaat actagatgcg taaaggagaa 901 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac 961 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa 1021 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt 1081 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag 1141 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac 1201 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta 1261 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat 1321 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc 1381 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat 1441 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct 1501 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca 1561 gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta gagcaatacg 1621 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 1681 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 1741 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 1801 acaatttcac acatactaga gaaagaggag aaatactaga tgaaaaacat aaatgccgac 1861 gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa 1921 tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat 1981 cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg 2041 caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc 2101 aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca 2161 aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat 2221 acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata 2281 gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat 2341 aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa 2401 aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta 2461 ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca 2521 acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac 2581 tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa 2641 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2701 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //