LOCUS BBa_J100016 2771 bp DNA linear 26-Dec-2024 DEFINITION 2 Clause 2-SAT Problem with Frameshifted LuxI and a GFP Reporter ACCESSION BBa_J100016 VERSION BBa_J100016.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 misc_feature 219..221 /label=Start misc_feature 865..870 /label=Stop misc_feature 222..229 /label=AsiSI Site misc_feature 231..255 /label=SAT Problem misc_feature 260..267 /label=AscI Site CDS 219..870 /label=BBa_J100014 RBS 879..893 /label=RBS-1\Strong RBS 886..890 /label=RBS misc_feature 879..893 /label=BBa_B0030 RBS 879..893 /label=BBa_B0030 CDS 900..1619 /label=GFP protein CDS 900..1619 /label=BBa_E0040 CDS 1628..1715 /label=end of LacI coding region (inactive) misc_feature 1628..1827 /label=BBa_R0010 misc_feature 1716..1753 /label=CAP binding site promoter 1764..1769 /label=-35 promoter 1788..1793 /label=-10 misc_feature 1793..1827 /label=LacI binding site misc_feature 1800..1800 /label=start promoter 1628..1827 /label=BBa_R0010 misc_feature 1840..1843 /label=conserved RBS 1836..1847 /label=BBa_B0034 misc_feature 1854..1855 /label=prefix misc_feature 2027..2027 /label=T misc_feature 2345..2345 /label=A CDS 1854..2603 /label=luxR misc_feature 1854..2609 /label=BBa_C0062 misc_feature 2610..2634 /label=Help:Barcodes CDS 1854..2609 /label=BBa_C0062 stem_loop 2654..2697 /label=stem_loop misc_feature 2643..2722 /label=BBa_B0010 terminator 2643..2722 /label=BBa_B0010 stem_loop 2738..2757 /label=T7 TE misc_feature 2764..2764 /label=stop polyA_site 2758..2771 /label=polya misc_feature 2731..2771 /label=BBa_B0012 terminator 2731..2771 /label=BBa_B0012 terminator 2643..2771 /label=BBa_B0015 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgca ccactgctag 241 ttctcggtcg ccacttctgg gcgcgccgca attgcaagcg gtcgtgcaac cattatgatt 301 aaaaaaagcg attttctggc cattccgagc gaagaatata aaggtattct gagcctgcgc 361 tatcaggttt ttaaacagcg tctggaatgg gatctggtgg ttgaaaataa tctggaaagt 421 gatgaatatg ataatagcaa tgccgaatat atttatgcct gtgatgatac cgaaaatgtt 481 agcggttgtt ggcgtctgct gccgaccacc ggtgattata tgctgaaaag cgtttttccg 541 gaactgctgg gtcagcagag cgcaccgaaa gatccgaata ttgttgaact gagccgtttt 601 gccgtgggta aaaatagcag caaaattaat aatagcgcca gcgaaattac catgaaactg 661 tttgaagcca tttataaaca tgccgttagc cagggtatta ccgaatatgt taccgttacc 721 agcaccgcaa ttgaacgttt tctgaaacgt attaaagtgc cgtgtcatcg tattggcgat 781 aaagaaattc atgttctggg cgataccaaa agcgttgttc tgagcatgcc gattaatgaa 841 cagtttaaaa aagccgtgct gaactaataa tactagagat taaagaggag aaatactaga 901 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg 961 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa 1021 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg 1081 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc 1141 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca 1201 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta 1261 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat 1321 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa 1381 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc 1441 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc 1501 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc 1561 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat 1621 actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 1681 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 1741 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 1801 attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac tagatgaaaa 1861 acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata 1921 atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac 1981 tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc 2041 ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag 2101 attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa 2161 ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta 2221 gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa 2281 aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc 2341 cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt 2401 taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata 2461 tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa 2521 tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa 2581 ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag 2641 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2701 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 2761 ctgcgtttat a //