##gff-version 3
##sequence-region BBa_J100016 1 2771
BBa_J100016	.	CDS	219	870	.	+	0	ID=BBa_J100014;Name=BBa_J100014
BBa_J100016	.	start_codon	219	221	.	+	0	ID=annotation2122243;Name=Start;Parent=BBa_J100014
BBa_J100016	.	stop_codon	865	870	.	+	0	ID=annotation2122244;Name=Stop;Parent=BBa_J100014
BBa_J100016	.	sequence_feature	231	255	.	+	0	ID=annotation2122246;Name=SAT Problem;Parent=BBa_J100014
BBa_J100016	.	sequence_feature	222	229	.	+	0	ID=annotation2122245;Name=AsiSI Site;Parent=BBa_J100014
BBa_J100016	.	sequence_feature	260	267	.	+	0	ID=annotation2122247;Name=AscI Site;Parent=BBa_J100014
BBa_J100016	.	ribosome_entry_site	1836	1847	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J100016	.	conserved_region	1840	1843	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J100016	.	CDS	900	1619	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J100016	.	CDS	900	1619	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J100016	.	terminator	2643	2771	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J100016	.	terminator	2731	2771	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J100016	.	polyA_site	2758	2771	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100016	.	engineered_region	2731	2771	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100016	.	stem_loop	2738	2757	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100016	.	stop_codon	2764	2764	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100016	.	terminator	2643	2722	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J100016	.	stem_loop	2654	2697	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100016	.	engineered_region	2643	2722	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100016	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J100016	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J100016	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100016	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100016	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100016	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100016	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J100016	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100016	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100016	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J100016	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J100016	.	ribosome_entry_site	879	893	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J100016	.	ribosome_entry_site	879	893	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J100016	.	ribosome_entry_site	886	890	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J100016	.	engineered_region	879	893	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J100016	.	CDS	1854	2609	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J100016	.	CDS	1854	2603	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J100016	.	engineered_tag	2610	2634	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J100016	.	engineered_region	1854	2609	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J100016	.	point_mutation	2027	2027	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J100016	.	engineered_region	1854	1855	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J100016	.	point_mutation	2345	2345	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J100016	.	promoter	1628	1827	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J100016	.	start_codon	1800	1800	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J100016	.	CDS	1628	1715	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J100016	.	promoter	1788	1793	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J100016	.	non_covalent_binding_site	1793	1827	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J100016	.	non_covalent_binding_site	1716	1753	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J100016	.	engineered_region	1628	1827	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J100016	.	promoter	1764	1769	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J100016	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J100016	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J100016	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J100016	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J100016	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J100016	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_J100016
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcaccactgc
tagttctcggtcgccacttctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttc
tggccattccgagcgaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatct
ggtggttgaaaataatctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaa
aatgttagcggttgttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtc
agcagagcgcaccgaaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataa
tagcgccagcgaaattaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgtt
accgttaccagcaccgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattc
atgttctgggcgataccaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaata
atactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttg
aattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttac
ccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaa
tgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaa
gaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatag
aatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacac
aatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaa
gcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacct
gtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgct
gggattacacatggcatggatgaactatacaaataataatactagagcaatacgcaaaccgcctctccccgcgcgttgg
ccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagtt
agctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataaca
atttcacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaata
aaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatatta
tttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggagg
caatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaatt
ggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcac
tgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactat
atagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataa
atatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaag
ctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactc
aatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaat
aacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata