BBa_J100017
type: ComponentDefinition
persistent identity: BBa J100017
display id: BBa_J100017
version: 1
was generated by: iGEM to SBOL conversion
description: TT+pLux+RBS+LuxI(2-SAT 2 clause)+RBS+GFP+pLac+RBS+LuxR+tRNAs
created: 2011-07-06T11:00:00Z
modified: 2015-08-31T04:08:21Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: TT+pLux+RBS+LuxI(2-SAT 2 clause)+RBS+GFP+pLac+RBS+LuxR+tRNAs
discontinued: false
dominant: false
group u list: _578_
m user id: 0
owner id: 5111
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J100016
component: BBa_J100011
sequence annotation: BBa_J100016
sequence annotation: BBa_J100011
sequence: https://synbiohub.org/public/igem/BBa_J100017_sequence/1

BBa_J100016
type: Component
persistent identity: component2318383
display id: component2318383
version: 1
top level: BBa_J100017
definition: BBa_J100016
access: public

BBa_J100011
type: Component
persistent identity: component2318402
display id: component2318402
version: 1
top level: BBa_J100017
definition: BBa_J100011
access: public

BBa_J100016
type: SequenceAnnotation
persistent identity: annotation2318383
display id: annotation2318383
version: 1
top level: BBa_J100017
location: https://synbiohub.org/public/igem/BBa_J100017/annotation2318383/range2318383/1
component: BBa_J100016

https://synbiohub.org/public/igem/BBa_J100017/annotation2318383/range2318383/1
type: Range
persistent identity: range2318383
display id: range2318383
version: 1
top level: BBa_J100017
direction: direction/reverse
start: 1
end: 2771
orientation: inline

BBa_J100011
type: SequenceAnnotation
persistent identity: annotation2318402
display id: annotation2318402
version: 1
top level: BBa_J100017
location: https://synbiohub.org/public/igem/BBa_J100017/annotation2318402/range2318402/1
component: BBa_J100011

https://synbiohub.org/public/igem/BBa_J100017/annotation2318402/range2318402/1
type: Range
persistent identity: range2318402
display id: range2318402
version: 1
top level: BBa_J100017
direction: direction/reverse
start: 2780
end: 3395
orientation: inline

BBa_J100011
type: ComponentDefinition
persistent identity: BBa J100011
display id: BBa_J100011
version: 1
was generated by: iGEM to SBOL conversion
description: pLpp-tRNA CCACU-pLpp-tRNA CUAGU-pLpp-tRNA CGGUC
created: 2011-06-08T11:00:00Z
modified: 2015-08-31T04:08:21Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: Produces suppressor tRNAs CCACU, CUAGU, and CGGUC, each under regulation of the lpp promoter.
discontinued: false
dominant: false
group u list: _578_
m user id: 0
owner id: 5111
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_S04334
component: BBa_S04336
component: BBa_S04333
sequence annotation: BBa_S04333
sequence annotation: BBa_S04336
sequence annotation: BBa_S04334
sequence: https://synbiohub.org/public/igem/BBa_J100011_sequence/1

BBa_S04334
type: Component
persistent identity: component2120821
display id: component2120821
version: 1
top level: BBa_J100011
definition: BBa_S04334
access: public

BBa_S04336
type: Component
persistent identity: component2120815
display id: component2120815
version: 1
top level: BBa_J100011
definition: BBa_S04336
access: public

BBa_S04333
type: Component
persistent identity: component2120809
display id: component2120809
version: 1
top level: BBa_J100011
definition: BBa_S04333
access: public

BBa_S04333
type: SequenceAnnotation
persistent identity: annotation2120809
display id: annotation2120809
version: 1
top level: BBa_J100011
location: https://synbiohub.org/public/igem/BBa_J100011/annotation2120809/range2120809/1
component: BBa_S04333

https://synbiohub.org/public/igem/BBa_J100011/annotation2120809/range2120809/1
type: Range
persistent identity: range2120809
display id: range2120809
version: 1
top level: BBa_J100011
direction: direction/reverse
start: 1
end: 200
orientation: inline

BBa_S04336
type: SequenceAnnotation
persistent identity: annotation2120815
display id: annotation2120815
version: 1
top level: BBa_J100011
location: https://synbiohub.org/public/igem/BBa_J100011/annotation2120815/range2120815/1
component: BBa_S04336

https://synbiohub.org/public/igem/BBa_J100011/annotation2120815/range2120815/1
type: Range
persistent identity: range2120815
display id: range2120815
version: 1
top level: BBa_J100011
direction: direction/reverse
start: 209
end: 408
orientation: inline

BBa_S04334
type: SequenceAnnotation
persistent identity: annotation2120821
display id: annotation2120821
version: 1
top level: BBa_J100011
location: https://synbiohub.org/public/igem/BBa_J100011/annotation2120821/range2120821/1
component: BBa_S04334

https://synbiohub.org/public/igem/BBa_J100011/annotation2120821/range2120821/1
type: Range
persistent identity: range2120821
display id: range2120821
version: 1
top level: BBa_J100011
direction: direction/reverse
start: 417
end: 616
orientation: inline

BBa_J100016
type: ComponentDefinition
persistent identity: BBa J100016
display id: BBa_J100016
version: 1
was generated by: iGEM to SBOL conversion
description: 2 Clause 2-SAT Problem with Frameshifted LuxI and a GFP Reporter
created: 2011-06-20T11:00:00Z
modified: 2015-08-31T04:08:21Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: GFP is produced only if the frameshifts in LuxI are suppressed, forming a 2 clause 2-SAT problem.
discontinued: false
dominant: false
experience: experience/None
group u list: _578_
m user id: 0
owner id: 5111
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
component: BBa_E0040
component: BBa_B0030
component: BBa_J100014
component: BBa_R0062
component: BBa_B0015
component: BBa_C0062
sequence annotation: BBa_B0015
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_J100014
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_R0010
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_J100016_sequence/1

BBa_B0034
type: Component
persistent identity: component2298205
display id: component2298205
version: 1
top level: BBa_J100016
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2298197
display id: component2298197
version: 1
top level: BBa_J100016
definition: LacI
access: public

BBa_E0040
type: Component
persistent identity: component2298196
display id: component2298196
version: 1
top level: BBa_J100016
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component2298193
display id: component2298193
version: 1
top level: BBa_J100016
definition: BBa_B0030
access: public

BBa_J100014
type: Component
persistent identity: component2298191
display id: component2298191
version: 1
top level: BBa_J100016
definition: BBa_J100014
access: public

BBa_B0034
type: Component
persistent identity: component2298185
display id: component2298185
version: 1
top level: BBa_J100016
definition: BBa_B0034
access: public

BBa_R0062
type: Component
persistent identity: component2298180
display id: component2298180
version: 1
top level: BBa_J100016
definition: lux pR
access: public

BBa_B0015
type: Component
persistent identity: component2298178
display id: component2298178
version: 1
top level: BBa_J100016
definition: BBa_B0015
access: public

BBa_B0015
type: Component
persistent identity: component2298218
display id: component2298218
version: 1
top level: BBa_J100016
definition: BBa_B0015
access: public

BBa_C0062
type: Component
persistent identity: component2298208
display id: component2298208
version: 1
top level: BBa_J100016
definition: luxr
access: public

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2298178
display id: annotation2298178
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298178/range2298178/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J100016/annotation2298178/range2298178/1
type: Range
persistent identity: range2298178
display id: range2298178
version: 1
top level: BBa_J100016
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2298180
display id: annotation2298180
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298180/range2298180/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J100016/annotation2298180/range2298180/1
type: Range
persistent identity: range2298180
display id: range2298180
version: 1
top level: BBa_J100016
direction: direction/forward
start: 138
end: 192
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2298185
display id: annotation2298185
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298185/range2298185/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J100016/annotation2298185/range2298185/1
type: Range
persistent identity: range2298185
display id: range2298185
version: 1
top level: BBa_J100016
direction: direction/reverse
start: 201
end: 212
orientation: inline

BBa_J100014
type: SequenceAnnotation
persistent identity: annotation2298191
display id: annotation2298191
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298191/range2298191/1
component: BBa_J100014

https://synbiohub.org/public/igem/BBa_J100016/annotation2298191/range2298191/1
type: Range
persistent identity: range2298191
display id: range2298191
version: 1
top level: BBa_J100016
direction: direction/reverse
start: 219
end: 870
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2298193
display id: annotation2298193
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298193/range2298193/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J100016/annotation2298193/range2298193/1
type: Range
persistent identity: range2298193
display id: range2298193
version: 1
top level: BBa_J100016
direction: direction/forward
start: 879
end: 893
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2298196
display id: annotation2298196
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298196/range2298196/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J100016/annotation2298196/range2298196/1
type: Range
persistent identity: range2298196
display id: range2298196
version: 1
top level: BBa_J100016
direction: direction/reverse
start: 900
end: 1619
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2298197
display id: annotation2298197
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298197/range2298197/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J100016/annotation2298197/range2298197/1
type: Range
persistent identity: range2298197
display id: range2298197
version: 1
top level: BBa_J100016
direction: direction/forward
start: 1628
end: 1827
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2298205
display id: annotation2298205
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298205/range2298205/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J100016/annotation2298205/range2298205/1
type: Range
persistent identity: range2298205
display id: range2298205
version: 1
top level: BBa_J100016
direction: direction/reverse
start: 1836
end: 1847
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2298208
display id: annotation2298208
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298208/range2298208/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_J100016/annotation2298208/range2298208/1
type: Range
persistent identity: range2298208
display id: range2298208
version: 1
top level: BBa_J100016
direction: direction/forward
start: 1854
end: 2609
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2298218
display id: annotation2298218
version: 1
top level: BBa_J100016
location: https://synbiohub.org/public/igem/BBa_J100016/annotation2298218/range2298218/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J100016/annotation2298218/range2298218/1
type: Range
persistent identity: range2298218
display id: range2298218
version: 1
top level: BBa_J100016
direction: direction/reverse
start: 2643
end: 2771
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_S04333
type: ComponentDefinition
persistent identity: BBa S04333
display id: BBa_S04333
version: 1
was generated by: iGEM to SBOL conversion
description: K199106:K199002
created: 2009-11-18T12:00:00Z
modified: 2015-05-08T01:14:38Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 5562
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Mary Gearing
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K199002
component: BBa_K199106
sequence annotation: BBa_K199106
sequence annotation: BBa_K199002
sequence: https://synbiohub.org/public/igem/BBa_S04333_sequence/1

BBa_K199002
type: Component
persistent identity: component2062783
display id: component2062783
version: 1
top level: BBa_S04333
definition: BBa_K199002
access: public

BBa_K199106
type: Component
persistent identity: component2062779
display id: component2062779
version: 1
top level: BBa_S04333
definition: BBa_K199106
access: public

BBa_K199106
type: SequenceAnnotation
persistent identity: annotation2062779
display id: annotation2062779
version: 1
top level: BBa_S04333
location: https://synbiohub.org/public/igem/BBa_S04333/annotation2062779/range2062779/1
component: BBa_K199106

https://synbiohub.org/public/igem/BBa_S04333/annotation2062779/range2062779/1
type: Range
persistent identity: range2062779
display id: range2062779
version: 1
top level: BBa_S04333
direction: direction/reverse
start: 1
end: 49
orientation: inline

BBa_K199002
type: SequenceAnnotation
persistent identity: annotation2062783
display id: annotation2062783
version: 1
top level: BBa_S04333
location: https://synbiohub.org/public/igem/BBa_S04333/annotation2062783/range2062783/1
component: BBa_K199002

https://synbiohub.org/public/igem/BBa_S04333/annotation2062783/range2062783/1
type: Range
persistent identity: range2062783
display id: range2062783
version: 1
top level: BBa_S04333
direction: direction/reverse
start: 58
end: 200
orientation: inline

BBa_S04334
type: ComponentDefinition
persistent identity: BBa S04334
display id: BBa_S04334
version: 1
was generated by: iGEM to SBOL conversion
description: K199106:K199028
created: 2009-11-18T12:00:00Z
modified: 2015-05-08T01:14:38Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 5562
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Mary Gearing
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K199028
component: BBa_K199106
sequence annotation: BBa_K199106
sequence annotation: BBa_K199028
sequence: https://synbiohub.org/public/igem/BBa_S04334_sequence/1

BBa_K199028
type: Component
persistent identity: component2062788
display id: component2062788
version: 1
top level: BBa_S04334
definition: BBa_K199028
access: public

BBa_K199106
type: Component
persistent identity: component2062784
display id: component2062784
version: 1
top level: BBa_S04334
definition: BBa_K199106
access: public

BBa_K199106
type: SequenceAnnotation
persistent identity: annotation2062784
display id: annotation2062784
version: 1
top level: BBa_S04334
location: https://synbiohub.org/public/igem/BBa_S04334/annotation2062784/range2062784/1
component: BBa_K199106

https://synbiohub.org/public/igem/BBa_S04334/annotation2062784/range2062784/1
type: Range
persistent identity: range2062784
display id: range2062784
version: 1
top level: BBa_S04334
direction: direction/reverse
start: 1
end: 49
orientation: inline

BBa_K199028
type: SequenceAnnotation
persistent identity: annotation2062788
display id: annotation2062788
version: 1
top level: BBa_S04334
location: https://synbiohub.org/public/igem/BBa_S04334/annotation2062788/range2062788/1
component: BBa_K199028

https://synbiohub.org/public/igem/BBa_S04334/annotation2062788/range2062788/1
type: Range
persistent identity: range2062788
display id: range2062788
version: 1
top level: BBa_S04334
direction: direction/reverse
start: 58
end: 200
orientation: inline

BBa_S04336
type: ComponentDefinition
persistent identity: BBa S04336
display id: BBa_S04336
version: 1
was generated by: iGEM to SBOL conversion
description: K199106:K199001
created: 2009-11-18T12:00:00Z
modified: 2015-05-08T01:14:38Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 5562
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Mary Gearing
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K199001
component: BBa_K199106
sequence annotation: BBa_K199106
sequence annotation: BBa_K199001
sequence: https://synbiohub.org/public/igem/BBa_S04336_sequence/1

BBa_K199001
type: Component
persistent identity: component2062798
display id: component2062798
version: 1
top level: BBa_S04336
definition: BBa_K199001
access: public

BBa_K199106
type: Component
persistent identity: component2062794
display id: component2062794
version: 1
top level: BBa_S04336
definition: BBa_K199106
access: public

BBa_K199106
type: SequenceAnnotation
persistent identity: annotation2062794
display id: annotation2062794
version: 1
top level: BBa_S04336
location: https://synbiohub.org/public/igem/BBa_S04336/annotation2062794/range2062794/1
component: BBa_K199106

https://synbiohub.org/public/igem/BBa_S04336/annotation2062794/range2062794/1
type: Range
persistent identity: range2062794
display id: range2062794
version: 1
top level: BBa_S04336
direction: direction/reverse
start: 1
end: 49
orientation: inline

BBa_K199001
type: SequenceAnnotation
persistent identity: annotation2062798
display id: annotation2062798
version: 1
top level: BBa_S04336
location: https://synbiohub.org/public/igem/BBa_S04336/annotation2062798/range2062798/1
component: BBa_K199001

https://synbiohub.org/public/igem/BBa_S04336/annotation2062798/range2062798/1
type: Range
persistent identity: range2062798
display id: range2062798
version: 1
top level: BBa_S04336
direction: direction/reverse
start: 58
end: 200
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_J100014
type: ComponentDefinition
persistent identity: BBa J100014
display id: BBa_J100014
version: 1
was generated by: iGEM to SBOL conversion
description: LuxI with 2 Clause 2-SAT Problem
created: 2011-06-20T11:00:00Z
modified: 2015-08-31T04:08:21Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: LuxI is frameshifted by the addition of a SAT problem between the engineered restriction sites AsiSI and AscI. The correct reading frame is restored only if suppressor tRNA CCACU (K199002) or suppressor tRNA CUAGU (K199001) AND suppressor tRNA CGGUC (K199028) or suppressor tRNA CCACU (K199002) are present in the cell.
discontinued: false
dominant: false
group u list: _578_
m user id: 0
owner id: 5111
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start
sequence annotation: AsiSI Site
sequence annotation: SAT Problem
sequence annotation: AscI Site
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_J100014_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2122243
display id: annotation2122243
version: 1
top level: BBa_J100014
location: https://synbiohub.org/public/igem/BBa_J100014/annotation2122243/range2122243/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J100014/annotation2122243/range2122243/1
type: Range
persistent identity: range2122243
display id: range2122243
version: 1
top level: BBa_J100014
direction: direction/forward
start: 1
end: 3
orientation: inline

AsiSI Site
type: SequenceAnnotation
persistent identity: annotation2122245
display id: annotation2122245
version: 1
top level: BBa_J100014
location: https://synbiohub.org/public/igem/BBa_J100014/annotation2122245/range2122245/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J100014/annotation2122245/range2122245/1
type: Range
persistent identity: range2122245
display id: range2122245
version: 1
top level: BBa_J100014
direction: direction/forward
start: 4
end: 11
orientation: inline

SAT Problem
type: SequenceAnnotation
persistent identity: annotation2122246
display id: annotation2122246
version: 1
top level: BBa_J100014
location: https://synbiohub.org/public/igem/BBa_J100014/annotation2122246/range2122246/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J100014/annotation2122246/range2122246/1
type: Range
persistent identity: range2122246
display id: range2122246
version: 1
top level: BBa_J100014
direction: direction/forward
start: 13
end: 37
orientation: inline

AscI Site
type: SequenceAnnotation
persistent identity: annotation2122247
display id: annotation2122247
version: 1
top level: BBa_J100014
location: https://synbiohub.org/public/igem/BBa_J100014/annotation2122247/range2122247/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J100014/annotation2122247/range2122247/1
type: Range
persistent identity: range2122247
display id: range2122247
version: 1
top level: BBa_J100014
direction: direction/forward
start: 42
end: 49
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2122244
display id: annotation2122244
version: 1
top level: BBa_J100014
location: https://synbiohub.org/public/igem/BBa_J100014/annotation2122244/range2122244/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J100014/annotation2122244/range2122244/1
type: Range
persistent identity: range2122244
display id: range2122244
version: 1
top level: BBa_J100014
direction: direction/forward
start: 647
end: 652
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K199001
type: ComponentDefinition
persistent identity: BBa K199001
display id: BBa_K199001
version: 1
was generated by: iGEM to SBOL conversion
description: CUAGU tRNA Suppressor (Produces Serine)
created: 2009-06-25T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
owned by: james
owned by: myers
mutable description: This gene encodes for a 5 base pair tRNA suppressor that suppresses CUAGU, which would normally cause a frame shift mutation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5109
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the 9 base anticodon loop that is complementary to the 5 base pair codon CUAGU.
star: false
creator: Romina Clemente
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5' Context
sequence annotation: Anticodon Loop
sequence annotation: 3' Context
sequence: https://synbiohub.org/public/igem/BBa_K199001_sequence/1

5' Context
type: SequenceAnnotation
persistent identity: annotation2006704
display id: annotation2006704
version: 1
top level: BBa_K199001
location: https://synbiohub.org/public/igem/BBa_K199001/annotation2006704/range2006704/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199001/annotation2006704/range2006704/1
type: Range
persistent identity: range2006704
display id: range2006704
version: 1
top level: BBa_K199001
direction: direction/forward
start: 1
end: 11
orientation: inline

Anticodon Loop
type: SequenceAnnotation
persistent identity: annotation2006705
display id: annotation2006705
version: 1
top level: BBa_K199001
location: https://synbiohub.org/public/igem/BBa_K199001/annotation2006705/range2006705/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199001/annotation2006705/range2006705/1
type: Range
persistent identity: range2006705
display id: range2006705
version: 1
top level: BBa_K199001
direction: direction/forward
start: 44
end: 52
orientation: inline

3' Context
type: SequenceAnnotation
persistent identity: annotation2006706
display id: annotation2006706
version: 1
top level: BBa_K199001
location: https://synbiohub.org/public/igem/BBa_K199001/annotation2006706/range2006706/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199001/annotation2006706/range2006706/1
type: Range
persistent identity: range2006706
display id: range2006706
version: 1
top level: BBa_K199001
direction: direction/forward
start: 104
end: 143
orientation: inline

BBa_K199002
type: ComponentDefinition
persistent identity: BBa K199002
display id: BBa_K199002
version: 1
was generated by: iGEM to SBOL conversion
description: CCACU tRNA Suppressor (Produces Serine)
created: 2009-06-25T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
owned by: james
owned by: myers
mutable description: This gene encodes for a 5 base pair tRNA suppressor that suppresses CCACU, which would normally cause a frame shift mutation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5109
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the 9 base anticodon loop that is complementary to the 5 base pair codon CCACU.
star: false
creator: Shamita Punjabi
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: 5' Context
sequence annotation: Anticodon Loop
sequence annotation: 3' Context
sequence: https://synbiohub.org/public/igem/BBa_K199002_sequence/1

5' Context
type: SequenceAnnotation
persistent identity: annotation2006707
display id: annotation2006707
version: 1
top level: BBa_K199002
location: https://synbiohub.org/public/igem/BBa_K199002/annotation2006707/range2006707/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199002/annotation2006707/range2006707/1
type: Range
persistent identity: range2006707
display id: range2006707
version: 1
top level: BBa_K199002
direction: direction/forward
start: 1
end: 11
orientation: inline

Anticodon Loop
type: SequenceAnnotation
persistent identity: annotation2006708
display id: annotation2006708
version: 1
top level: BBa_K199002
location: https://synbiohub.org/public/igem/BBa_K199002/annotation2006708/range2006708/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199002/annotation2006708/range2006708/1
type: Range
persistent identity: range2006708
display id: range2006708
version: 1
top level: BBa_K199002
direction: direction/forward
start: 44
end: 52
orientation: inline

3' Context
type: SequenceAnnotation
persistent identity: annotation2006709
display id: annotation2006709
version: 1
top level: BBa_K199002
location: https://synbiohub.org/public/igem/BBa_K199002/annotation2006709/range2006709/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199002/annotation2006709/range2006709/1
type: Range
persistent identity: range2006709
display id: range2006709
version: 1
top level: BBa_K199002
direction: direction/forward
start: 104
end: 143
orientation: inline

BBa_K199028
type: ComponentDefinition
persistent identity: BBa K199028
display id: BBa_K199028
version: 1
was generated by: iGEM to SBOL conversion
description: CGGUC tRNA Suppressor (Produces Serine)
created: 2009-07-08T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
owned by: james
owned by: myers
mutable description: This gene encodes for a 5 base pair tRNA suppressor that suppresses CGGUC, which would normally cause a frame shift mutation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5117
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the 9 base anticodon loop that is complementary to the 5 base pair codon CGGUC.
star: false
creator: Alyndria Thompson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5' context
sequence annotation: anticodon loop
sequence annotation: 3
sequence: https://synbiohub.org/public/igem/BBa_K199028_sequence/1

5' context
type: SequenceAnnotation
persistent identity: annotation2010431
display id: annotation2010431
version: 1
top level: BBa_K199028
location: https://synbiohub.org/public/igem/BBa_K199028/annotation2010431/range2010431/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199028/annotation2010431/range2010431/1
type: Range
persistent identity: range2010431
display id: range2010431
version: 1
top level: BBa_K199028
direction: direction/forward
start: 1
end: 11
orientation: inline

anticodon loop
type: SequenceAnnotation
persistent identity: annotation2010430
display id: annotation2010430
version: 1
top level: BBa_K199028
location: https://synbiohub.org/public/igem/BBa_K199028/annotation2010430/range2010430/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199028/annotation2010430/range2010430/1
type: Range
persistent identity: range2010430
display id: range2010430
version: 1
top level: BBa_K199028
direction: direction/forward
start: 44
end: 52
orientation: inline

3
type: SequenceAnnotation
persistent identity: annotation2010432
display id: annotation2010432
version: 1
top level: BBa_K199028
location: https://synbiohub.org/public/igem/BBa_K199028/annotation2010432/range2010432/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199028/annotation2010432/range2010432/1
type: Range
persistent identity: range2010432
display id: range2010432
version: 1
top level: BBa_K199028
direction: direction/forward
start: 104
end: 143
orientation: inline

BBa_K199106
type: ComponentDefinition
persistent identity: BBa K199106
display id: BBa_K199106
version: 1
was generated by: iGEM to SBOL conversion
description: pLpp promoter
created: 2009-11-18T12:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: The sequence matches that naturally found in E. coli.
owned by: james
owned by: myers
mutable description: The lpp promoter is one of the strongest promoters in E. coli.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5562
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Mary Gearing
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K199106_sequence/1

https://synbiohub.org/public/igem/BBa_J100017_sequence/1
type: Sequence
persistent identity: BBa J100017 sequence
display id: BBa_J100017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcaccactgctagttctcggtcgccacttctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J100011_sequence/1
type: Sequence
persistent identity: BBa J100011 sequence
display id: BBa_J100011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtttactagacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J100016_sequence/1
type: Sequence
persistent identity: BBa J100016 sequence
display id: BBa_J100016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcaccactgctagttctcggtcgccacttctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04333_sequence/1
type: Sequence
persistent identity: BBa S04333 sequence
display id: BBa_S04333_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04334_sequence/1
type: Sequence
persistent identity: BBa S04334 sequence
display id: BBa_S04334_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04336_sequence/1
type: Sequence
persistent identity: BBa S04336 sequence
display id: BBa_S04336_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtttactagacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J100014_sequence/1
type: Sequence
persistent identity: BBa J100014 sequence
display id: BBa_J100014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgatcgcaccactgctagttctcggtcgccacttctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199001_sequence/1
type: Sequence
persistent identity: BBa K199001 sequence
display id: BBa_K199001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtttactagacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199002_sequence/1
type: Sequence
persistent identity: BBa K199002 sequence
display id: BBa_K199002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199028_sequence/1
type: Sequence
persistent identity: BBa K199028 sequence
display id: BBa_K199028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199106_sequence/1
type: Sequence
persistent identity: BBa K199106 sequence
display id: BBa_K199106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z