LOCUS       BBa_J100028              877 bp    DNA     linear       16-Feb-2025
DEFINITION  placeholder insert for BsaI Golden Gate Assembly of promoter
ACCESSION   BBa_J100028
VERSION     BBa_J100028.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=prefix
     misc_feature    858..877
                     /label=suffix
     RBS             158..169
                     /label=B0034
     misc_feature    23..28
                     /label=BbsI cuts left
     misc_feature    176..178
                     /label=start
     misc_feature    851..856
                     /label=double stop
     misc_feature    34..39
                     /label=BsaI cuts left
     misc_feature    29..32
                     /label=misc
     misc_feature    135..140
                     /label=BsaI cuts right
     misc_feature    142..145
                     /label=misc
     misc_feature    146..151
                     /label=BbsI cuts right
     CDS             176..856
                     /label=mRFP E1010
ORIGIN
        1 gaattcgcgg ccgcttctag aggtcttccg actgagacct cacactggct caccttcggg
       61 tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg
      121 cttttttatt atttggtctc agcgggaaga caactagaaa gaggagaaat actagatggc
      181 ttcctccgaa gatgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      241 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      301 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      361 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      421 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagatgg
      481 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
      541 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
      601 ttgggaagct tccaccgaac gtatgtaccc ggaagatggt gctctgaaag gtgaaatcaa
      661 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
      721 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
      781 ctcccacaac gaagattaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
      841 caccggtgct taataatact agtagcggcc gctgcag
//