LOCUS BBa_J100041 2803 bp DNA linear 26-Dec-2024 DEFINITION LuxI/GFP with 3 clause 2-SAT problem ACCESSION BBa_J100041 VERSION BBa_J100041.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 misc_feature 222..229 /label=AsiSI Site misc_feature 292..299 /label=AscI Site CDS 219..902 /label=BBa_J100040 RBS 911..925 /label=RBS-1\Strong RBS 918..922 /label=RBS misc_feature 911..925 /label=BBa_B0030 RBS 911..925 /label=BBa_B0030 CDS 932..1651 /label=GFP protein CDS 932..1651 /label=BBa_E0040 CDS 1660..1747 /label=end of LacI coding region (inactive) misc_feature 1660..1859 /label=BBa_R0010 misc_feature 1748..1785 /label=CAP binding site promoter 1796..1801 /label=-35 promoter 1820..1825 /label=-10 misc_feature 1825..1859 /label=LacI binding site misc_feature 1832..1832 /label=start promoter 1660..1859 /label=BBa_R0010 misc_feature 1872..1875 /label=conserved RBS 1868..1879 /label=BBa_B0034 misc_feature 1886..1887 /label=prefix misc_feature 2059..2059 /label=T misc_feature 2377..2377 /label=A CDS 1886..2635 /label=luxR misc_feature 1886..2641 /label=BBa_C0062 misc_feature 2642..2666 /label=Help:Barcodes CDS 1886..2641 /label=BBa_C0062 stem_loop 2686..2729 /label=stem_loop misc_feature 2675..2754 /label=BBa_B0010 terminator 2675..2754 /label=BBa_B0010 stem_loop 2770..2789 /label=T7 TE misc_feature 2796..2796 /label=stop polyA_site 2790..2803 /label=polya misc_feature 2763..2803 /label=BBa_B0012 terminator 2763..2803 /label=BBa_B0012 terminator 2675..2803 /label=BBa_B0015 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgcg ccactgctag 241 tgtaataatt ctagtgcggt cgtaataatt ccactgcggt cgtaataatt gggcgcgccg 301 caattgcaag cggtcgtgca accattatga ttaaaaaaag cgattttctg gccattccga 361 gcgaagaata taaaggtatt ctgagcctgc gctatcaggt ttttaaacag cgtctggaat 421 gggatctggt ggttgaaaat aatctggaaa gtgatgaata tgataatagc aatgccgaat 481 atatttatgc ctgtgatgat accgaaaatg ttagcggttg ttggcgtctg ctgccgacca 541 ccggtgatta tatgctgaaa agcgtttttc cggaactgct gggtcagcag agcgcaccga 601 aagatccgaa tattgttgaa ctgagccgtt ttgccgtggg taaaaatagc agcaaaatta 661 ataatagcgc cagcgaaatt accatgaaac tgtttgaagc catttataaa catgccgtta 721 gccagggtat taccgaatat gttaccgtta ccagcaccgc aattgaacgt tttctgaaac 781 gtattaaagt gccgtgtcat cgtattggcg ataaagaaat tcatgttctg ggcgatacca 841 aaagcgttgt tctgagcatg ccgattaatg aacagtttaa aaaagccgtg ctgaactaat 901 aatactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg 961 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 1021 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 1081 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 1141 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 1201 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 1261 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 1321 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 1381 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 1441 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 1501 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 1561 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 1621 acatggcatg gatgaactat acaaataata atactagagc aatacgcaaa ccgcctctcc 1681 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 1741 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 1801 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat 1861 actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat 1921 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat 1981 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt 2041 taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga 2101 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat 2161 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga 2221 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg 2281 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt 2341 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat 2401 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc 2461 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg 2521 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca 2581 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata 2641 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 2701 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2761 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //