##gff-version 3
##sequence-region BBa_J100041 1 2803
BBa_J100041	.	CDS	219	902	.	+	0	ID=BBa_J100040;Name=BBa_J100040
BBa_J100041	.	sequence_feature	222	229	.	+	0	ID=annotation2127710;Name=AsiSI Site;Parent=BBa_J100040
BBa_J100041	.	sequence_feature	292	299	.	+	0	ID=annotation2127711;Name=AscI Site;Parent=BBa_J100040
BBa_J100041	.	CDS	1886	2641	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J100041	.	point_mutation	2377	2377	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J100041	.	CDS	1886	2635	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J100041	.	engineered_region	1886	2641	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J100041	.	engineered_tag	2642	2666	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J100041	.	point_mutation	2059	2059	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J100041	.	engineered_region	1886	1887	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J100041	.	terminator	2675	2803	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J100041	.	terminator	2675	2754	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J100041	.	engineered_region	2675	2754	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100041	.	stem_loop	2686	2729	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100041	.	terminator	2763	2803	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J100041	.	engineered_region	2763	2803	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100041	.	stop_codon	2796	2796	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100041	.	stem_loop	2770	2789	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100041	.	polyA_site	2790	2803	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100041	.	ribosome_entry_site	911	925	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J100041	.	engineered_region	911	925	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J100041	.	ribosome_entry_site	918	922	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J100041	.	ribosome_entry_site	911	925	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J100041	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J100041	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J100041	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J100041	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J100041	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J100041	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J100041	.	ribosome_entry_site	1868	1879	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J100041	.	conserved_region	1872	1875	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J100041	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J100041	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J100041	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100041	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100041	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J100041	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100041	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100041	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100041	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100041	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J100041	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J100041	.	CDS	932	1651	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J100041	.	CDS	932	1651	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J100041	.	promoter	1660	1859	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J100041	.	non_covalent_binding_site	1748	1785	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J100041	.	promoter	1820	1825	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J100041	.	engineered_region	1660	1859	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J100041	.	CDS	1660	1747	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J100041	.	non_covalent_binding_site	1825	1859	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J100041	.	promoter	1796	1801	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J100041	.	start_codon	1832	1832	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
>BBa_J100041
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcgccactgc
tagtgtaataattctagtgcggtcgtaataattccactgcggtcgtaataattgggcgcgccgcaattgcaagcggtcg
tgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgctat
caggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaatg
ccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattatat
gctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgtttt
gccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaac
atgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaagt
gccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaat
gaacagtttaaaaaagccgtgctgaactaataatactagagattaaagaggagaaatactagatgcgtaaaggagaaga
acttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagag
ggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaa
cacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaa
gagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaa
gtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttg
gacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaa
cttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgat
ggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacc
acatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagag
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacat
aaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatct
gatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatattt
caatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtaga
ttattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgta
attaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgc
ttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgt
tccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaa
gaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtca
ctttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacagg
agcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata