LOCUS BBa_J100043 2821 bp DNA linear 16-Feb-2025 DEFINITION LuxI/GFP with 3 clause 3-SAT problem ACCESSION BBa_J100043 VERSION BBa_J100043.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 CDS 219..920 /label=BBa_J100042 RBS 929..943 /label=RBS-1\Strong RBS 936..940 /label=RBS misc_feature 929..943 /label=BBa_B0030 RBS 929..943 /label=BBa_B0030 CDS 950..1669 /label=GFP protein CDS 950..1669 /label=BBa_E0040 CDS 1678..1765 /label=end of LacI coding region (inactive) misc_feature 1678..1877 /label=BBa_R0010 misc_feature 1766..1803 /label=CAP binding site promoter 1814..1819 /label=-35 promoter 1838..1843 /label=-10 misc_feature 1843..1877 /label=LacI binding site misc_feature 1850..1850 /label=start promoter 1678..1877 /label=BBa_R0010 misc_feature 1890..1893 /label=conserved RBS 1886..1897 /label=BBa_B0034 misc_feature 1904..1905 /label=prefix misc_feature 2077..2077 /label=T misc_feature 2395..2395 /label=A CDS 1904..2653 /label=luxR misc_feature 1904..2659 /label=BBa_C0062 misc_feature 2660..2684 /label=Help:Barcodes CDS 1904..2659 /label=BBa_C0062 stem_loop 2704..2747 /label=stem_loop misc_feature 2693..2772 /label=BBa_B0010 terminator 2693..2772 /label=BBa_B0010 stem_loop 2788..2807 /label=T7 TE misc_feature 2814..2814 /label=stop polyA_site 2808..2821 /label=polya misc_feature 2781..2821 /label=BBa_B0012 terminator 2781..2821 /label=BBa_B0012 terminator 2693..2821 /label=BBa_B0015 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgcg ccactgctag 241 tgccatcgta ataattctag tgcggtctcc atcgtaataa ttccactgcc atctctactg 301 taataattgg gcgcgccgca attgcaagcg gtcgtgcaac cattatgatt aaaaaaagcg 361 attttctggc cattccgagc gaagaatata aaggtattct gagcctgcgc tatcaggttt 421 ttaaacagcg tctggaatgg gatctggtgg ttgaaaataa tctggaaagt gatgaatatg 481 ataatagcaa tgccgaatat atttatgcct gtgatgatac cgaaaatgtt agcggttgtt 541 ggcgtctgct gccgaccacc ggtgattata tgctgaaaag cgtttttccg gaactgctgg 601 gtcagcagag cgcaccgaaa gatccgaata ttgttgaact gagccgtttt gccgtgggta 661 aaaatagcag caaaattaat aatagcgcca gcgaaattac catgaaactg tttgaagcca 721 tttataaaca tgccgttagc cagggtatta ccgaatatgt taccgttacc agcaccgcaa 781 ttgaacgttt tctgaaacgt attaaagtgc cgtgtcatcg tattggcgat aaagaaattc 841 atgttctggg cgataccaaa agcgttgttc tgagcatgcc gattaatgaa cagtttaaaa 901 aagccgtgct gaactaataa tactagagat taaagaggag aaatactaga tgcgtaaagg 961 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 1021 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 1081 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 1141 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt 1201 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg 1261 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga 1321 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa 1381 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa 1441 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 1501 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 1561 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 1621 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcaa 1681 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 1741 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 1801 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 1861 gataacaatt tcacacatac tagagaaaga ggagaaatac tagatgaaaa acataaatgc 1921 cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata atgatattaa 1981 tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac tcgcgatcat 2041 ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc ctaaaaaatg 2101 gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag attattctaa 2161 ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa ataaaaaatc 2221 tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta gtttccctat 2281 tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa aagacaacta 2341 tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc cttctctagt 2401 tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt taaccaaaag 2461 agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata tttcaaaaat 2521 attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa 2581 tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa ttgattgccc 2641 atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag agccaggcat 2701 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2761 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2821 a //